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Targeting Smurf1 to block PDK1–Akt signaling in KRAS-mutated colorectal cancer
Targeting Smurf1 to block PDK1–Akt signaling in KRAS-mutated colorectal cancer
The phosphoinositide 3-kinase (PI3K)–Akt axis is one of the most frequently activated pathways and is demonstrated a...
Unlocking saponin biosynthesis in soapwort
Unlocking saponin biosynthesis in soapwort
Soapwort (Saponaria officinalis) is a flowering plant from the Caryophyllaceae family with a long history of human use as ...
Genetically engineered synthetic cells activate cargo release upon temperature shift
Genetically engineered synthetic cells activate cargo release upon temperature shift
We combine RNA thermometer genetic switches, cell-free protein expression and synthetic cell design to create cell-sized s...
Splice-modifying drug mechanisms
Splice-modifying drug mechanisms
A new study combines massively parallel assays, transcriptomics and biophysical modeling to provide a framework for analyz...
Direct RAS inhibitors turn 10
Direct RAS inhibitors turn 10
RAS proteins, central drivers of cancer, appeared ‘undruggable’ for almost 30 years. Here we provide a persona...
Metabolic determinants of germinal center B cell formation and responses
Metabolic determinants of germinal center B cell formation and responses
Germinal center (GC) B cells are crucial for the generation of GCs and long-lived humoral immunity. Here we report that on...
Phospholipid biosynthesis modulates nucleotide metabolism and reductive capacity
Phospholipid biosynthesis modulates nucleotide metabolism and reductive capacity
Phospholipid and nucleotide syntheses are fundamental metabolic processes in eukaryotic organisms, with their dysregulatio...
Restoring susceptibility to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus
Restoring susceptibility to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus
Infections by Staphylococcus aureus have been treated historically with β-lactam antibiotics. However, these antibiot...
Stress it up
AKT is a serine/threonine kinase that is essential for cellular metabolism and proliferation and is overactive in cancer c...
Reeling in microorganisms
The metabolic engineering of microorganisms is an attractive platform for bioremediation and the industrial production of ...
Switching off the stress response
Cells respond to adverse environmental changes by activating stress response pathways. However, the continuous activation ...
Profiling the proximal proteome of the activated μ-opioid receptor
Profiling the proximal proteome of the activated μ-opioid receptor
The μ-opioid receptor (μOR) represents an important target of therapeutic and abused drugs. So far, most underst...
Targeting the chromatin binding of exportin-1 disrupts NFAT and T cell activation
Targeting the chromatin binding of exportin-1 disrupts NFAT and T cell activation
Exportin-1 (XPO1/CRM1) plays a central role in the nuclear-to-cytoplasmic transport of hundreds of proteins and contribute...
The death gaze of MEDUSA
The death gaze of MEDUSA
Chemogenetic profiling can reveal genetic determinants that coordinate phenotypic responses to therapeutics, along with pr...
Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression
Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression
Telomere dysfunction is intricately linked to the aging process and stands out as a prominent cancer hallmark. Here we dem...
Engineering APOBEC3A deaminase for highly accurate and efficient base editing
Engineering APOBEC3A deaminase for highly accurate and efficient base editing
Cytosine base editors (CBEs) are effective tools for introducing C-to-T base conversions, but their clinical applications ...
Biocatalytic cyclization of small macrolactams by a penicillin-binding protein-type thioesterase
Biocatalytic cyclization of small macrolactams by a penicillin-binding protein-type thioesterase
Macrocyclic peptides represent promising scaffolds for chemical tools and potential therapeutics. Synthetic methods for pe...
Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
Compact Cas9 nucleases hold great promise for therapeutic applications. Although several compact Cas9 nucleases have been ...
In search of chemical rationales
In search of chemical rationales
Small molecules and drugs are not homogenously distributed across cells, and are instead enriched in distinct subcellular ...
Author Correction: An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity
Authors and AffiliationsDepartment of Chemistry, The University of Chicago, Chicago, IL, USATong Wu, Chang Liu, Siyuan Zou...
Characterizing the metabolomes of phase-separated condensates
Characterizing the metabolomes of phase-separated condensates
Cells contain compartments composed of phase-separated protein condensates. We find that these condensates have a unique c...
Medicine from modified mRNA
Medicine from modified mRNA
As the COVID-19 pandemic rapidly spread around the world, the scientific community desperately searched for therapeutic in...
Survival of the sweetest tooth
The development of multidrug resistance in bacteria is a global public health concern, and the genetic and molecular deter...
Traffic control
Lysosomal degradation of extracellular and membrane proteins can be facilitated by lysosome-targeted chimeras (LYTACs). LY...
RNA-based anti-CRISPRs
RNA-based anti-CRISPRs
Using a type I-F SRU as an example, Camara-Wilpert et al. demonstrate that SRUs inhibit CRISPR–Cas immunity via an RNA-bas...
Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides
Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides
The modular nature of nonribosomal peptide biosynthesis has driven efforts to generate peptide analogs by substituting ami...
Accelerating the design of pili-enabled living materials using an integrative technological workflow
Accelerating the design of pili-enabled living materials using an integrative technological workflow
Bacteria can be programmed to create engineered living materials (ELMs) with self-healing and evolvable functionalities. H...
An allosteric redox switch involved in oxygen protection in a CO2 reductase
An allosteric redox switch involved in oxygen protection in a CO2 reductase
Metal-dependent formate dehydrogenases reduce CO2 with high efficiency and selectivity, but are usually very oxygen sensit...
A single-component, light-assisted uncaging switch for endoproteolytic release
A single-component, light-assisted uncaging switch for endoproteolytic release
Proteases function as pivotal molecular switches, initiating numerous biological events. Notably, potyviral protease, deri...
Mechanistic plasticity in ApmA enables aminoglycoside promiscuity for resistance
Mechanistic plasticity in ApmA enables aminoglycoside promiscuity for resistance
The efficacy of aminoglycoside antibiotics is waning due to the acquisition of diverse resistance mechanisms by bacteria. ...
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