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Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays
In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inac...
Development and optimisation of influenza C and influenza D pseudotyped viruses
To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (P...
Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1
Free-living birds face various health risks due to viruses, such as avian influenza, West Nile Virus, and paramyxoviruses ...
Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses
Since its emergence in 1996, highly pathogenic avian influenza (HPAI) viruses of the A/Goose/Guangdong/1/96 lineage have d...
Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany
Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the...
Curcumin inhibits HIV-1 by modulating FOXP3 and suppressing CCR5 via PI3K/AKT and JAK/STAT pathways
Acquired Immune Deficiency Syndrome (AIDS) is caused by infection with the human immunodeficiency virus (HIV). The viral a...
In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection
Dorsal root ganglion (DRG) neurons, located in the dorsal root of spinal nerves, serve as critical relays for transmitting...
Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells
While genome editing has been established in chickens, where cultured primordial germ cell (PGC) systems are available, th...
Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer
Persistent infection with high-risk human papillomavirus (hrHPV) causes almost all cases of cervical cancer. Despite the s...
Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)
Tilapia Lake Virus (TiLV) is a newly emerging, highly infectious pathogen in aquaculture. It has been reported to affect t...
Evaluation of dead-end hollowfiber ultrafilter options for enumerating somatic and F+ coliphage in ambient waters and wastewater
The discharge of improperly treated wastewater can contaminate receiving waterbodies. Within these waterbodies, exposure t...
Development of a quadruple qPCR assay for simultaneous detection of four common bovine pathogens
Bovines play a crucial role in global agriculture and livestock production. However, outbreaks of infectious diseases in c...
Evaluating the effectiveness of a novel environmental decontamination system utilizing low-energy hyper-charged photoelectrons against coronavirus
The COVID-19 pandemic had profound economic and social effects across the globe. The present study evaluated the virus att...
Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples
Virophages are small double-stranded DNA (dsDNA) viruses that parasitize the replication machinery of giant viruses, addin...
Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania
BackgroundCytomegalovirus (CMV) remains a significant infection in neonates and its early detection can aid with further t...
Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces
Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-bo...
Evaluation of two IgG-scFv bispecific antibodies for neutralizing Omicron variants of SARS-CoV-2
Melissa García-Vega: Validation, Methodology, Formal analysis. Verónica Mata-Haro: Writing – review & editing, Validat...
Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples
The global emergence of SARS-CoV-2 has highlighted the need for rapid, sensitive, and affordable diagnostic tools, not onl...
A dual-target real-time PCR for proactive detection of Mpox variants
Monkeypox virus (MPXV) is a zoonotic infection that can be spread human-to-human (Lim et al., 2023). It has two main clade...
Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP
Luis Galipienso: Supervision, Investigation, Formal analysis, Conceptualization. Luis Rubio: Writing – review & editin...
Environments contaminated by pesticides are hotspots for the selection of bacterial chassis for biotechnological applications
Herbicides represent the most extensively utilized category of pesticides globally and are designed to damage or eliminate...
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics
Parasitic nematodes are globally important pathogens of animals, humans and plants. The accurate identification and charac...
Chemical imaging of lignocellulosic biomass: Mapping plant chemistry
Lignocellulosic Biomass (LB) encompasses a variety of plant samples like trees (both softwood and hardwood), grasses as cu...
Multigene engineering in plants: Technologies, applications, and future prospects
The emerging bioeconomy presents a promising solution to both economic and environmental challenges. Within the bioeconomy...
Towards syngas biorefineries: The potential of microbial consortia for syngas valorisation
Gasification has emerged as a promising platform to cope with recalcitrant organic waste within the framework of biomass-b...
Advances on the biosynthesis of ergothioneine using microbial chassis
Ergothioneine (ERG) is a naturally occurring sulfur-containing amino acid with a unique structure. Due to its potent antio...
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis
3,4-HPAA3,4-hydroxyphenylacetaldehyde4-HPAA4-hydroxyphenylacetaldehyde4-HPAC4-hydroxyphenylacetic acid4-HPP4-hydroxyphenyl...
Advances in nanopore direct RNA sequencing and its impact on biological research
Nanopore direct RNA sequencing (DRS) is a transformative technology that enables full-length, single-molecule sequencing o...
Intersecting precision fermentation for global cell-based food production innovation: Challenges and opportunities
Cell-based food production relies on cultivating animals, plants, or microorganisms in a controlled environment to produce...
Engineered microbial production of carotenoids and their cleavage products: Recent advances and prospects
Carotenoids are a diverse group of tetraterpenoid compounds containing 40 carbon atoms commonly found in plants, fungi, ba...
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