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Innovative nanosensing methods for lysozyme identification
Lysozyme (LYZ) is a crucial enzyme recognized for its remarkable antimicrobial properties, serving as a key component of t...
Rosmarinic acid production using advanced metabolic engineering strategies
Rosmarinic acid (RA) is a high-value natural compound, which was initially isolated from Rosmarinus officinalis L. in 1958...
Homologous expression and characterization of Coniochaeta ligniaria glycoside hydrolase family 115 α-glucuronidase
Xylan is the major hemicellulose present within most dedicated energy crops and agricultural residues, comprising between ...
De Novo enzyme design of a minimal and soluble carbonic anhydrase from Mesorhizobium loti assisted by molecular dynamics simulation
CA is one of the most ancient enzymes on Earth, widely distributed across both eukaryotic and prokaryotic organisms [1]. T...
Strong alone, weak together: biofilm tensile strength in kangaroo rat burrows
Desert kangaroo rats (Dipodomys deserti) construct burrows that create protected micro-niches favorable to increased micro...
A thermostable cellobiose phosphorylase from Thermoclostridium caenicola and its application to in vitro biotransformation
Lignocellulose is the most abundant renewable biomass resource on Earth, and its efficient conversion is a part of the cir...
Mechanism of tyrosine 69 in the flavin domain modulating electron transfer efficiency in BVU5 azoreductase
Azo dyes constitute major pollutants in textile dyeing wastewater, where cleavage of the azo bond (-N = N-) represents the...
Preparation of magnetic xylanase cross-linked aggregates (m-CLXAs) for the hydrolysis of arabinoxylan by mechano-enzymology
The development of a biocatalyst consisting of immobilised xylanases as cross-linked enzyme aggregates (CLEAs) on magnetic...
Enhanced synthesis of alanyl-glutamine dipeptide via molecular modification and process optimization of α-amino acid ester acyltransferase EAET
Glutamine (L-Gln) is a conditionally essential amino acid that plays a crucial role in various physiological processes, pa...
Identification of a thermostable GH6 family cellulase from chaetomium thermophilum exhibiting high cellobiose and ionic liquid tolerance
Lignocellulosic biomass can be readily obtained from plants, seaweeds, and agricultural waste and is a beneficial resource...
Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation
Growing global concerns about climate change, CO2 reduction, and fossil fuel depletion have stimulated interest in a circu...
An H1N1 virus biosensor based on enzyme activity-gated PER-CRISPR/Cas12a cascade signal amplification
According to data from the World Health Organization, every year, 250，000–500，000 people die from influenza worldwide [1]....
Anti-biofilm efficiency and substrate specificity of recombinantly produced human paraoxonases
Human paraoxonases are multifunctional enzymes considered to be antioxidant, anti-inflammatory, antimicrobial, and anti-at...
Expression and biochemical characterization of wild-type and mutant β-amylases from Priestia flexa in Komagataella phaffii
Starch is one of the most abundant polysaccharide in nature and serves as a substrate for the production of numerous valua...
Metabolic engineering of Bacillus subtilis for enhanced p-Coumaric acid production and antimicrobial applications
p-Coumaric acid (p-CA), also known as 4-hydroxycinnamic acid, is mainly found in herbal plants, as well as fruits and vege...
Highly efficient spermidine production system in Escherichia coli BL21 (DE3) based on precursor metabolic modules optimization and carboxyaminopropylagmatine pathway construction
N-(3-Aminopropyl)-1,4-butanediamine, also known as spermidine, is a naturally occurring polyamine compound ubiquitously pr...
Microbial-catalyzed biotransformation of Soyasapogenol B by Streptomyces griseus ATCC 13273, and Penicillium griseofulvum CICC 40293
Soyasapogenol B, an aglycone part (non-sugar) of group B soyasaponin, pentacyclic triterpenoids (PTs) [1], [2], [3], [4], ...
Development of high-efficiency hybrid strains for cellulolytic enzyme production via interspecific and intergeneric protoplast fusion of Trichoderma and Penicillium species
Approximately 10 billion tons of dry plant matter are produced globally each year. Over half of it is composed of cellulos...
Study on bio-imprinted Aspergillus niger lipase cross-linked aggregates and catalytic synthesis of Vitamin E succinate
Vitamin E is a vital nutrient for human health that has been widely exploited in the food and pharmaceutical industries [1...
Overexpression, biochemical characterization, and structural modeling of polyhydroxybutyrate depolymerase from Nocardiopsis dassonvillei
Plastic pollution is steadily increasing as a major environmental problem. The global plastic production was approximately...
Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays
In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inac...
Development and optimisation of influenza C and influenza D pseudotyped viruses
To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (P...
Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1
Free-living birds face various health risks due to viruses, such as avian influenza, West Nile Virus, and paramyxoviruses ...
Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses
Since its emergence in 1996, highly pathogenic avian influenza (HPAI) viruses of the A/Goose/Guangdong/1/96 lineage have d...
Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany
Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the...
Curcumin inhibits HIV-1 by modulating FOXP3 and suppressing CCR5 via PI3K/AKT and JAK/STAT pathways
Acquired Immune Deficiency Syndrome (AIDS) is caused by infection with the human immunodeficiency virus (HIV). The viral a...
In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection
Dorsal root ganglion (DRG) neurons, located in the dorsal root of spinal nerves, serve as critical relays for transmitting...
Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells
While genome editing has been established in chickens, where cultured primordial germ cell (PGC) systems are available, th...
Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer
Persistent infection with high-risk human papillomavirus (hrHPV) causes almost all cases of cervical cancer. Despite the s...
Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)
Tilapia Lake Virus (TiLV) is a newly emerging, highly infectious pathogen in aquaculture. It has been reported to affect t...
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