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Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method
Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method
Seamless site-directed mutagenesis is an important technique for studying protein functions, tuning enzyme catalytic activ...
Using clusterProfiler to characterize multiomics data
Using clusterProfiler to characterize multiomics data
With the advent of multiomics, software capable of multidimensional enrichment analysis has become increasingly crucial fo...
A multi-glycomic platform for the analysis of food carbohydrates
A multi-glycomic platform for the analysis of food carbohydrates
Carbohydrates comprise the largest fraction of most diets and exert a profound impact on health. Components such as simple...
Preamplification-free viral RNA diagnostics with single-nucleotide resolution using MARVE, an origami paper-based colorimetric nucleic acid test
Preamplification-free viral RNA diagnostics with single-nucleotide resolution using MARVE, an origami paper-based colorimetric nucleic acid test
The evolution and mutation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgent concerns as they pos...
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics
FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics
Rare cells have an important role in development and disease, and methods for isolating and studying cell subsets are ther...
Analysis of nanomaterial biocoronas in biological and environmental surroundings
Analysis of nanomaterial biocoronas in biological and environmental surroundings
A biomolecular coating, or biocorona, forms on the surface of engineered nanomaterials (ENMs) immediately as they enter bi...
Surface nanocoating of bacteria as a versatile platform to develop living therapeutics
Surface nanocoating of bacteria as a versatile platform to develop living therapeutics
Bacteria have been extensively utilized as living therapeutics for disease treatment due to their unique characteristics, ...
In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis
In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis
Human early embryonic development is the cornerstone of a healthy baby. Abnormal early embryonic development may lead to d...
Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils
Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils
Eosinophils are a class of granulocytes with pleiotropic functions in homeostasis and various human diseases. Nevertheless...
An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells
An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells
Pigs share anatomical and physiological traits with humans and can serve as a large-animal model for translational medicin...
Light-mediated intracellular polymerization
Light-mediated intracellular polymerization
The synthesis of synthetic intracellular polymers offers groundbreaking possibilities in cellular biology and medical rese...
Pan-evolutionary and regulatory genome architecture delineated by an integrated macro- and microsynteny approach
Pan-evolutionary and regulatory genome architecture delineated by an integrated macro- and microsynteny approach
The forthcoming massive genome data generated by the Earth BioGenome Project will open up a new era of comparative genomic...
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS
BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS
Genetic interactions have the potential to modulate phenotypes, including human disease. In principle, genome-wide associa...
Generating human bone marrow organoids for disease modeling and drug discovery
Generating human bone marrow organoids for disease modeling and drug discovery
The bone marrow supports and regulates hematopoiesis, responding to physiological requirements for blood cell production o...
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CO2
Isotopically carbon-labeled α-amino acids are valuable synthetic targets that are increasingly needed in pharmacology...
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
A promising cell-therapy approach for heart failure aims at differentiating human pluripotent stem cells (hPSCs) into func...
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice
Head-mounted central venous access during optical recordings and manipulations of neural activity in mice
Establishing reliable intravenous catheterization in mice with optical implants allows the combination of neural manipulat...
In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation
In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation
The central nervous system (CNS) comprises diverse and morphologically complex cells. To understand the molecular basis of...
Synthesis and functionalization of scalable and versatile 2D protein films via amyloid-like aggregation
Synthesis and functionalization of scalable and versatile 2D protein films via amyloid-like aggregation
Two-dimensional (2D) protein films can be used to modify the properties of surfaces, and find applications predominantly i...
Small RNA structural biochemistry in a post-sequencing era
Small RNA structural biochemistry in a post-sequencing era
High-throughput sequencing has had an enormous impact on small RNA research during the past decade. However, sequencing on...
Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins
Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins
RNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decodin...
Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst
Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst
The tail-to-head terpene (THT) cyclization is a biochemical process that gives rise to many terpene natural product skelet...
A platform for whole-genome speed introgression from Aegilops tauschii to wheat for breeding future crops
A platform for whole-genome speed introgression from Aegilops tauschii to wheat for breeding future crops
Breeding new and sustainable crop cultivars of high yields and desirable traits has been a major challenge for ensuring fo...
CONIPHER: a computational framework for scalable phylogenetic reconstruction with error correction
CONIPHER: a computational framework for scalable phylogenetic reconstruction with error correction
Intratumor heterogeneity provides the fuel for the evolution and selection of subclonal tumor cell populations. However, a...
Author Correction: Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input
Author Correction: Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input
These authors contributed equally: Tetsuya Handa, Akihito Harada, Kazumitsu Maehara.Cell Biology Center, Institute of Inno...
Reproducible strategy for excisional skin-wound-healing studies in mice
Reproducible strategy for excisional skin-wound-healing studies in mice
Wound healing is a complex physiological process involving various cell types and signaling pathways. The capability to ob...
Robust scoring of selective drug responses for patient-tailored therapy selection
Robust scoring of selective drug responses for patient-tailored therapy selection
Most patients with advanced malignancies are treated with severely toxic, first-line chemotherapies. Personalized treatmen...
Analyzing somatic mutations by single-cell whole-genome sequencing
Analyzing somatic mutations by single-cell whole-genome sequencing
Somatic mutations are the cause of cancer and have been implicated in other, noncancerous diseases and aging. While clonal...
Ultra-fast supercritically solvothermal polymerization for large single-crystalline covalent organic frameworks
Ultra-fast supercritically solvothermal polymerization for large single-crystalline covalent organic frameworks
Crystalline polymer materials, e.g., hyper-crosslinked polystyrene, conjugate microporous polymers and covalent organic fr...
High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ
High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ
Bacteria often function as a community, called the microbiota, consisting of many different bacterial species. The accurat...
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