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Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays
In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inac...
Development and optimisation of influenza C and influenza D pseudotyped viruses
To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (P...
Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1
Free-living birds face various health risks due to viruses, such as avian influenza, West Nile Virus, and paramyxoviruses ...
Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses
Since its emergence in 1996, highly pathogenic avian influenza (HPAI) viruses of the A/Goose/Guangdong/1/96 lineage have d...
Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany
Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the...
Curcumin inhibits HIV-1 by modulating FOXP3 and suppressing CCR5 via PI3K/AKT and JAK/STAT pathways
Acquired Immune Deficiency Syndrome (AIDS) is caused by infection with the human immunodeficiency virus (HIV). The viral a...
In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection
Dorsal root ganglion (DRG) neurons, located in the dorsal root of spinal nerves, serve as critical relays for transmitting...
Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells
While genome editing has been established in chickens, where cultured primordial germ cell (PGC) systems are available, th...
Reference gene evaluation for digital PCR; Applications for RNA biomarker testing in cervical precancer
Persistent infection with high-risk human papillomavirus (hrHPV) causes almost all cases of cervical cancer. Despite the s...
Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)
Tilapia Lake Virus (TiLV) is a newly emerging, highly infectious pathogen in aquaculture. It has been reported to affect t...
Evaluation of dead-end hollowfiber ultrafilter options for enumerating somatic and F+ coliphage in ambient waters and wastewater
The discharge of improperly treated wastewater can contaminate receiving waterbodies. Within these waterbodies, exposure t...
Development of a quadruple qPCR assay for simultaneous detection of four common bovine pathogens
Bovines play a crucial role in global agriculture and livestock production. However, outbreaks of infectious diseases in c...
Evaluating the effectiveness of a novel environmental decontamination system utilizing low-energy hyper-charged photoelectrons against coronavirus
The COVID-19 pandemic had profound economic and social effects across the globe. The present study evaluated the virus att...
Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples
Virophages are small double-stranded DNA (dsDNA) viruses that parasitize the replication machinery of giant viruses, addin...
Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania
BackgroundCytomegalovirus (CMV) remains a significant infection in neonates and its early detection can aid with further t...
Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces
Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-bo...
Evaluation of two IgG-scFv bispecific antibodies for neutralizing Omicron variants of SARS-CoV-2
Melissa García-Vega: Validation, Methodology, Formal analysis. Verónica Mata-Haro: Writing – review & editing, Validat...
Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples
The global emergence of SARS-CoV-2 has highlighted the need for rapid, sensitive, and affordable diagnostic tools, not onl...
A dual-target real-time PCR for proactive detection of Mpox variants
Monkeypox virus (MPXV) is a zoonotic infection that can be spread human-to-human (Lim et al., 2023). It has two main clade...
Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP
Luis Galipienso: Supervision, Investigation, Formal analysis, Conceptualization. Luis Rubio: Writing – review & editin...
Comparison of direct RNA sequencing of Orthoavulavirus javaense using two different chemistries on the MinION platform
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The use of human intestinal enteroid cell cultures for detection of multiple gastroenteric viruses
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Improving the production of BaEV lentivirus by comprehensive optimization
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Enhancing lumpy skin disease control: Effective competitive and indirect ELISAs for serological surveillance
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Construction and verification of an infectious cDNA clone of encephalomyocarditis virus from pigs
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Rapid, inexpensive multiplex pathogen detection using resequencing microarrays
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Endothelial growth media components alter SARS-CoV-2 spike-directed growth kinetics
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Development of a urinary antigen test for BK Polyomavirus to help clinicians in patients’ follow-up
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Performance of a novel viral load assay for plasma HIV-1 RNA quantification compared with Roche real time PCR in China
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Impedance-based method for the quantification of infectious SARS-CoV-2
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