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Epitope profiling of cow's milk allergen-specific antibodies with determining IgE content in epitopes-ALL, a 14-epitopes mixture
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Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library
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Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells
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Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment
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Natural cross-reactive anti-SARS-CoV-2 antibodies in avian egg yolk
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Adaptation of the in vivo respiratory burst assay for fathead minnow larvae (Pimephales promelas)
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Characterization of an IL-8 cleavage inhibition assay to determine the functionality of anti-SpyCEP antibodies in human sera
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PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria
Chronic spontaneous urticaria (CSU) is a disorder where urticaria (hives) occurs without an identifiable provoking factor ...
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An immunochromatographic test for serological diagnosis of scrub typhus
Scrub typhus is an acute febrile human disease caused by mite vectors, which are infected by a gram negative bacterium O. ...
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Performance of Softcup® menstrual cup and vulvovaginal swab samples for detection and quantification of genital cytokines
The field of mucosal immunology research has expanded significantly over the years and understanding biomarkers as correla...
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High-resolution epitope mapping of commercial antibodies to ANCA antigens by yeast surface display
Epitope mapping provides critical insight into antibody-antigen interactions. Epitope mapping of autoantibodies from patie...
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Development and basic performance verification of a rapid homogeneous bioassay for agonistic antibodies against the thyroid-stimulating hormone receptor
KeywordsThyroid-stimulating antibodiesThyroid-stimulating hormone receptor antibodiesGraves' diseaseThyroid-stimulating ho...
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Selection of positive controls and their impact on anti-drug antibody assay performance
Biologic drugs have the potential to induce adaptive immune responses, resulting in the formation of anti-drug antibodies ...
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Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples
Premature lymphocytes develop into non-autoreactive, mature naïve CD4+ or CD8+ T cells in the thymus before entering the c...
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A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera
Volume 528, May 2024, 113652Author links open overlay panel, , , , , , Highlights•Streptococcus pyogenes infections requir...
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An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination
The T cell receptor (TCR)-CD3 complex consists of the antigen binding subunit TCRαβ and three CD3 heterodimers, including ...
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Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study
On March 13, 2021, Tunisia started a widespread immunization program against SARS-CoV-2 utilizing different vaccinations t...
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Magnetic CAR T cell purification using an anti-G4S linker antibody
Chimeric antigen receptor (CAR) redirected T cells are successfully employed in the combat against several hematological m...
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Simultaneous determination of inflammatory factors SAA and LTF based on stable element labeling and inductively coupled plasma mass spectrometry to aid in the diagnosis of infection
Serum amyloid A (SAA), discovered and named over 50 years ago, is a protein with a length of 150 kb and a size of 12,000 (...
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Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles
Complement, a crucial component of humoral innate immunity, plays a pivotal role in opsonizing nanopharmaceuticals and pat...
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Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment
Cookies are used by this site. Cookie SettingsAll content on this site: Copyright © 2024 Elsevier B.V., its licensors, an...
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Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction
The global escalation of antibiotic-resistant bacteria, intensified by the limited availability of new classes of antibiot...
Journal Of Immunological Methods
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Protein G affinity chromatography is an underrated but very potent purification method for a broad range of species-independent and tag-less Fab-fragments
Because of their superior properties for certain biological applications small antibody derivatives like fragment of antig...
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Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen
Intestinal transplantation (ITx) is the definitive treatment for intestinal failure (Sudan et al., 2000; Kaufman et al., 2...
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
Borrelia burgdorferi sensu lato (s.l.) is a group of gram-negative bacteria that are the etiological factor of Lyme diseas...
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Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen
Available online 30 November 2023, 113586Author links open overlay panel, , , , AbstractSevere acute respiratory syndrome ...
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Evaluation of HLA-DR expression in monocytes and CD64 in neutrophils as A predictor of SEPSIS/sirs in the infectious-inflammatory process
Sepsis is a systemic response of the body to an infection, often resulting in the dysfunction of multiple organs and the f...
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Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies
Characterization of the cell surface markers expressed by the immune cell population is critical for investigating the eff...
Journal Of Immunological Methods
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Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells
Available online 29 November 2023, 113588Author links open overlay panel, , , , , , , , , , , , , AbstractThe Enzyme-Linke...
Journal Of Immunological Methods
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Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN-ɣ enzyme-linked ImmunoSpot (ELISpot) protocols
Influenza viruses cause hundreds of thousands of deaths and millions of hospitalizations annually and remain a global heal...
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