AbuMadighem A, Shuchat S, Lunenfeld E, Yossifon G, Huleihel M (2022) Testis on a chip—a microfluidic three-dimensional culture system for the development of spermatogenesis in-vitro. Biofabrication 14(3):035004. https://doi.org/10.1088/1758-5090/ac6126
Almamoun R, Pierozan P, Karlsson O (2024) Mechanistic screening of reproductive toxicity in a novel 3D testicular co-culture model shows significant impairments following exposure to low-dibutyl phthalate concentrations. Arch Toxicol 98(8):2695–2709. https://doi.org/10.1007/s00204-024-03767-6
Article CAS PubMed PubMed Central Google Scholar
Alves-Lopes JP, Söder O, Stukenborg J-B (2017) Testicular organoid generation by a novel in vitro three-layer gradient system. Biomaterials 130:76–89. https://doi.org/10.1016/j.biomaterials.2017.03.025
Article CAS PubMed Google Scholar
de Angelis C, Galdiero M, Pivonello C et al (2017) The environment and male reproduction: the effect of cadmium exposure on reproductive function and its implication in fertility. Reprod Toxicol 73:105–127. https://doi.org/10.1016/j.reprotox.2017.07.021
Article CAS PubMed Google Scholar
Baert Y, De Kock J, Alves-Lopes JP, Söder O, Stukenborg J-B, Goossens E (2017) Primary human testicular cells self-organize into organoids with testicular properties. Stem Cell Reports 8(1):30–38. https://doi.org/10.1016/j.stemcr.2016.11.012
Article CAS PubMed Google Scholar
Cham T-C, Ibtisham F, Al-Dissi A, Honaramooz A (2024) An in vitro testicular organoid model for the study of testis morphogenesis, somatic cell maturation, endocrine function, and toxicological assessment of endocrine disruptors. Reprod Toxicol 128:108645. https://doi.org/10.1016/j.reprotox.2024.108645
Article CAS PubMed Google Scholar
Cohen AB, Nikmehr B, Abdelaal OA et al (2024) Microrna analysis of in vitro differentiation of spermatogonial stem cells using a 3D human testis organoid system. Biomedicines 12(8):1774. https://doi.org/10.3390/biomedicines12081774
Article CAS PubMed PubMed Central Google Scholar
Connor TH, Lawson CC, Polovich M, McDiarmid MA (2014) Reproductive health risks associated with occupational exposures to antineoplastic drugs in health care settings. J Occup Environ Med 56(9):901–910. https://doi.org/10.1097/Jom.0000000000000249
Article CAS PubMed PubMed Central Google Scholar
Cortez J, Leiva B, Torres CG et al (2022) Generation and characterization of bovine testicular organoids derived from primary somatic cell populations. Animals 12(17):2283. https://doi.org/10.3390/ani12172283
Article PubMed PubMed Central Google Scholar
Cui Y, Harteveld F, Ba Omar HAM et al (2024) Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients. Hum Reprod Open 2024:hoae049. https://doi.org/10.1093/hropen/hoae049
Article PubMed PubMed Central Google Scholar
Edmonds ME, Woodruff TK (2020) Testicular organoid formation is a property of immature somatic cells, which self-assemble and exhibit long-term hormone-responsive endocrine function. Biofabrication 12(4):671–687. https://doi.org/10.1088/1758-5090/ab9907
Enangue Njembele AN, Tremblay JJ (2021) Mechanisms of MEHP inhibitory action and analysis of potential replacement plasticizers on Leydig cell steroidogenesis. Int J Mol Sci 22(21):11456. https://doi.org/10.3390/ijms222111456
Article CAS PubMed PubMed Central Google Scholar
Fang X, Richa T, Nguyen VP, Richburg JH (2024) The blood-testis barrier disruption is a prerequisite for toxicant-induced peritubular macrophage increases in the testis of peripubertal rats. Toxicol Sci 200:70–78. https://doi.org/10.1093/toxsci/kfae043
Article CAS PubMed PubMed Central Google Scholar
Freires IA, Sardi JdCO, de Castro RD, Rosalen PL (2016) Alternative animal and non-animal models for drug discovery and development: bonus or burden? Pharm Res 34(4):681–686. https://doi.org/10.1007/s11095-016-2069-z
Article CAS PubMed Google Scholar
Freyberger A, Weimer M, Lofink W, Ahr H-J (2010) Short-term dynamic culture of rat testicular fragments as a model to assess effects on steroidogenesis—potential use and limitations. Reprod Toxicol 30(1):36–43. https://doi.org/10.1016/j.reprotox.2009.10.016
Article CAS PubMed Google Scholar
Galdon G, Zarandi NP, Deebel NA et al (2024) In vitro generation of haploid germ cells from human XY and XXY immature testes in a 3D organoid system. Bioengineering 11(7):677. https://doi.org/10.3390/bioengineering11070677
Article CAS PubMed PubMed Central Google Scholar
Ghaleno LR, Hajari MA, Choshali MA et al (2024) Hyaluronic acid-alginate hydrogel stimulates the differentiation of neonatal mouse testicular cells into hepatocyte-like and other cell lineages in three-dimensional culture. Biol Cell 116(10):e2400049. https://doi.org/10.1111/boc.202400049
Article CAS PubMed Google Scholar
Hofer M, Lutolf MP (2021) Engineering organoids. Nat Rev Mater 6(5):402–420. https://doi.org/10.1038/s41578-021-00279-y
Article CAS PubMed PubMed Central Google Scholar
Huang RF, Xia H, Meng T et al (2025) Construction of human pluripotent stem cell-derived testicular organoids and their use as humanized testis models for evaluating the effects of semaglutide. Theranostics 15(6):2597–2623. https://doi.org/10.7150/thno.104523
Article CAS PubMed PubMed Central Google Scholar
Ibtisham F et al (2017) Progress and future prospect of in vitro spermatogenesis. Oncotarget 8:66709–66727. https://doi.org/10.18632/oncotarget.19640
Article PubMed PubMed Central Google Scholar
Karamanos NK, Theocharis AD, Piperigkou Z et al (2021) A guide to the composition and functions of the extracellular matrix. FEBS J 288(24):6850–6912. https://doi.org/10.1111/febs.15776
Article CAS PubMed Google Scholar
Kiani M et al (2021) Formation of organoid-like structures in the decellularized rat testis. Iran J Basic Med Sci 24:1523–1528. https://doi.org/10.22038/IJBMS.2021.58294.12948
Article PubMed PubMed Central Google Scholar
Komeya M, Sato T, Ogawa T (2018) In vitro spermatogenesis: a century-long research journey, still half way around. Reprod Med Biol 17(4):407–420. https://doi.org/10.1002/rmb2.12225
Article PubMed PubMed Central Google Scholar
Levine H, Jørgensen N, Martino-Andrade A et al (2023) Temporal trends in sperm count: a systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries. Hum Reprod Update 29(2):157–176. https://doi.org/10.1093/humupd/dmac035
Li Y, Wu J, Ye P et al (2022) Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering. Bioactive Mater 10:15–31. https://doi.org/10.1016/j.bioactmat.2021.09.014
Li Z, Li Q, Zhou C et al (2023) Organoid-on-a-chip: current challenges, trends, and future scope toward medicine. Biomicrofluidics 17(5):051505. https://doi.org/10.1063/5.0171350
Article CAS PubMed PubMed Central Google Scholar
Li H, Wang X-R, Hu Y-F et al (2024) Advances in immunology of male reproductive toxicity induced by common environmental pollutants. Environ Int 190:108898. https://doi.org/10.1016/j.envint.2024.108898
Article CAS PubMed Google Scholar
Maschmeyer I, Goossens E, Marx U et al (2020)
Comments (0)