HK-2 cell response to TGF-β highly depends on cell culture medium formulations

Balzer MS, Rohacs T, Susztak K (2022) How many cell types are in the kidney and what do they do? Annual Rev Phyiol 84:507–531. https://doi.org/10.1146/annurev-physiol-052521-121841

Article  CAS  Google Scholar 

Bhattacharyya S, Wu M, Fang F, Tourtellotte W, Feghali-Bostwick C, Varga J (2011) Early growth response transcription factors: key mediators of fibrosis and novel targets for anti-fibrotic therapy. Matrix Biol 30(4):235–242. https://doi.org/10.1016/j.matbio.2011.03.005

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bhattacharyya S, Fang F, Tourtellotte W, Varga J (2013) Egr-1: new conductor for the tissue repair orchestra directs harmony (regeneration) or cacophony (fibrosis). J Pathol 229(2):286–297. https://doi.org/10.1002/path.4131

Article  CAS  PubMed  Google Scholar 

Bozic M, de Rooij J, Parisi E, Ortega MR, Fernandez E, Valdivielso JM (2011) Glutamatergic signaling maintains the epithelial phenotype of proximal tubular cells. J Am Soc Nephrol 22(6):1099–1111. https://doi.org/10.1681/ASN.2010070701

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bozic M, Caus MA, Rodrigues-Diez RA, Pedraza NA, Ruiz-Ortega M, Garí E, Gallel P, Panadés MJ, Martinez A, Fernández E, Valdivielso JM (2020) Protective role of renal proximal tubular alpha-synuclein in the pathogenesis of kidney fibrosis. Nat Commun 11(1):1943. https://doi.org/10.1038/s41467-020-15732-9

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brennan EP, Morine MJ, Walsh DW, Roxburgh SA, Lindenmeyer MT, Brazil DP, Gaora PO, Roche HM, Sadlier DM, Cohen CD, Consortium G, Godson C, Martin F (2012) Next-generation sequencing identifies TGF-beta1-associated gene expression profiles in renal epithelial cells reiterated in human diabetic nephropathy. Biochim Biophys Acta 4:589–599. https://doi.org/10.1016/j.bbadis.2012.01.008

Article  CAS  Google Scholar 

Cheng O, Thuillier R, Sampson E, Schultz G, Ruiz P, Zhang X, Yuen PST, Mannon RB (2006) Connective tissue growth factor is a biomarker and mediator of kidney allograft fibrosis. Am J Transplant 6(10):2292–2306. https://doi.org/10.1111/j.1600-6143.2006.01493.x

Article  CAS  PubMed  Google Scholar 

Franch HA, Shay JW, Alpern RJ, Preisig PA (1995) Involvement of pRB family in TGF beta-dependent epithelial cell hypertrophy. J Cell Biol 129(1):245–254. https://doi.org/10.1083/jcb.129.1.245

Article  CAS  PubMed  Google Scholar 

Gerritsma JS, Gerritsen AF, De Ley M, van Es LA, Daha MR (1997) Interferon-gamma induces biosynthesis of complement components C2, C4 and factor H by human proximal tubular epithelial cells. Cytokine 9(4):276–283. https://doi.org/10.1006/cyto.1996.0164

Article  CAS  PubMed  Google Scholar 

Gerritsma JSJ, van Kooten C, Gerritsen AF, van Es LA, Daha MR (1998) Transforming growth factor-β1 regulates chemokine and complement production by human proximal tubular epithelial cells. Kidney Int 53(3):609–616. https://doi.org/10.1046/j.1523-1755.1998.00799.x

Article  CAS  PubMed  Google Scholar 

Ghallab A, Seddek A (2020) PPARG as therapeutic target for antifibrotic therapy. EXCLI J 19:227–229. https://doi.org/10.17179/excli2020-1136

Article  PubMed  PubMed Central  Google Scholar 

Ho LC, Sung JM, Shen YT, Jheng HF, Chen SH, Tsai PJ, Tsai YS (2016) Egr-1 deficiency protects from renal inflammation and fibrosis. J Mol Med (berl) 94(8):933–942. https://doi.org/10.1007/s00109-016-1403-6

Article  CAS  PubMed  Google Scholar 

Jenkinson SE, Chung GW, van Loon E, Bakar NS, Dalzell AM, Brown CDA (2012) The limitations of renal epithelial cell line HK-2 as a model of drug transporter expression and function in the proximal tubule. Pflgers Arch 464(6):601–611. https://doi.org/10.1007/s00424-012-1163-2

Article  CAS  Google Scholar 

Kang HM, Lim JH, Noh KH, Park D, Cho HS, Susztak K, Jung CR (2019) Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies. Sci Rep 9(1):17610. https://doi.org/10.1038/s41598-019-53855-2

Article  CAS  PubMed  PubMed Central  Google Scholar 

Khundmiri SA, Chen LA, Lederer ED, Yang CR, Knepper MA (2021) Transcriptomes of major proximal tubule cell culture models. J Am Soc Nephrol 32(1):86–97. https://doi.org/10.1681/ASN.2020010009

Article  CAS  PubMed  Google Scholar 

Kökény G, Calvier L, Legchenko E, Chouvarine P, Mózes MM, Hansmann G (2020) PPARγ is a gatekeeper for extracellular matrix and vascular cell homeostasis: beneficial role in pulmonary hypertension and renal/cardiac/pulmonary fibrosis. Curr Opin Nephrol Hypertens 29(2):171–179. https://doi.org/10.1097/MNH.0000000000000580

Article  CAS  PubMed  Google Scholar 

Kökény G, Németh Á, Kopp JB, Chen W, Oler AJ, Manzéger A, Rosivall L, Mózes MM (2022) Susceptibility to kidney fibrosis in mice is associated with early growth response-2 protein and tissue inhibitor of metalloproteinase-1 expression. Kidney Int 102(2):337–354. https://doi.org/10.1016/j.kint.2022.03.029

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee JW, Chou CL, Knepper MA (2015) Deep sequencing in microdissected renal tubules identifies nephron segment-specific transcriptomes. J Am Soc Nephrol 26(11):2669–2677. https://doi.org/10.1681/ASN.2014111067

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li H, Ruan XZ, Powis SH, Fernando R, Mon WY, Wheeler DC, Moorhead JF, Varghese Z (2005) EPA and DHA reduce LPS-induced inflammation responses in HK-2 cells: evidence for a PPAR-gamma-dependent mechanism. Kidney Int 67(3):867–874. https://doi.org/10.1111/j.1523-1755.2005.00151.x

Article  CAS  PubMed  Google Scholar 

Li S, Zhao J, Huang R, Steiner T, Bourner M, Mitchell M, Thompson DC, Zhao B, Xia M (2017) Development and application of human renal proximal tubule epithelial cells for assessment of compound toxicity. Curr Chem Genom Transl Med 11:19–30. https://doi.org/10.2174/2213988501711010019

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu Y (2004) Epithelial to mesenchymal transition in renal fibrogenesis: pathologic significance, molecular mechanism, and therapeutic intervention. J Am Soc Nephrol 15(1):1–12. https://doi.org/10.1097/01.asn.0000106015.29070.e7

Article  CAS  PubMed  Google Scholar 

Németh Á, Mózes MM, Calvier L, Hansmann G, Kökény G (2019) The PPARγ agonist pioglitazone prevents TGF-β induced renal fibrosis by repressing EGR-1 and STAT3. BMC Nephrol 20(1):245. https://doi.org/10.1186/s12882-019-1431-x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Peake PW, O’Grady S, Pussell BA, Charlesworth JA (1999) C3a is made by proximal tubular HK-2 cells and activates them via the C3a receptor. Kidney Int 56(5):1729–1736. https://doi.org/10.1046/j.1523-1755.1999.00722.x

Article  CAS  PubMed  Google Scholar 

Puck TT, Cieciura SJ, Robinson A (1958) Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med 108(6):945–956. https://doi.org/10.1084/jem.108.6.945

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ryan MJ, Johnson G, Kirk J, Fuerstenberg SM, Zager RA, Torok-Storb B (1994) HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney. Kidney Int 45(1):48–57. https://doi.org/10.1038/ki.1994.6

Article  CAS  PubMed  Google Scholar 

Sanchez-Romero N, Schophuizen CM, Gimenez I, Masereeuw R (2016) In vitro systems to study nephropharmacology: 2D versus 3D models. Eur J Pharmacol 790:36–45. https://doi.org/10.1016/j.ejphar.2016.07.010

Article  CAS  PubMed  Google Scholar 

Sasaki N, Toyoda M, Hasegawa F, Fujiwara M, Gomi F, Ishiwata T (2019) Fetal bovine serum enlarges the size of human pancreatic cancer spheres accompanied by an increase in the expression of cancer stem cell markers. Biochem Biophys Res Commun 514(1):112–117. https://doi.org/10.1016/j.bbrc.2019.04.117

Article  CAS  PubMed  Google Scholar 

Sato Y, Takahashi M, Yanagita M (2020) Pathophysiology of AKI to CKD progression. Semin Nephrol 40(2):206–215. https://doi.org/10.1016/j.semnephrol.2020.01.011

Article  CAS  PubMed  Google Scholar 

Sun S, Ning X, Zhai Y, Du R, Lu Y, He L, Li R, Wu W, Sun W, Wang H (2014) Egr-1 mediates chronic hypoxia-induced renal interstitial fibrosis via the PKC/ERK pathway. Am J Nephrol 39(5):436–448. https://doi.org/10.1159/000362249

Article  CAS  PubMed  Google Scholar 

Tian YC, Phillips AO (2003) TGF-beta1-mediated inhibition of HK-2 cell migration. J Am Soc Nephrol 14(3):631–640. https://doi.org/10.1097/01.asn.0000053418.56286.5e

Article  CAS  PubMed  Google Scholar 

Valdés A, Lucio-Cazaña FJ, Castro-Puyana M, García-Pastor C, Fiehn O, Marina ML (2021) Comprehensive metabolomic study of the response of HK-2 cells to hyperglycemic hypoxic diabetic-like milieu. Sci Rep 11(1):5058. https://doi.org/10.1038/s41598-021-84590-2

Article  CAS  PubMed  PubMed Central 

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