Afzaal M, Saeed F, Shah YA, Hussain M, Rabail R, Socol CT, et al. Human gut microbiota in health and disease: unveiling the relationship. Front Microbiol. 2022;13:999001.

Article  PubMed  PubMed Central  Google Scholar 

Kaper JB, Nataro JP, Mobley HLT. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004;2:123–40.

Article  CAS  PubMed  Google Scholar 

Iacob S, Iacob DG, Luminos LM. Intestinal microbiota as a host defense mechanism to infectious threats. Front Microbiol. 2019;10:426119.

Google Scholar 

Khan I, Bai Y, Zha L, Ullah N, Ullah H, Shah SRH, et al. Mechanism of the Gut Microbiota Colonization Resistance and Enteric Pathogen infection. Front Cell Infect Microbiol. 2021;11:716299.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Takao M, Yen H, Tobe T. LeuO enhances butyrate-induced virulence expression through a positive regulatory loop in enterohaemorrhagic Escherichia coli. Mol Microbiol. 2014;93:1302–13.

Article  CAS  PubMed  Google Scholar 

Melton-Celsa AR. Shiga Toxin (Stx) classification, structure, and function. Microbiol Spectr. 2014;2:EHEC–0024.

Article  PubMed  Google Scholar 

Zumbrun SD, Melton-Celsa AR, Smith MA, Gilbreath JJ, Merrell DS, O’Brien AD. Dietary choice affects Shiga toxin-producing Escherichia coli (STEC) O157:H7 colonization and disease. Proc Natl Acad Sci U S A. 2013;110:E2126–33.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Roxas JL, Koutsouris A, Bellmeyer A, Tesfay S, Royan S, Falzari K, et al. Enterohemorrhagic E. Coli alters murine intestinal epithelial tight junction protein expression and barrier function in a Shiga toxin independent manner. Lab Invest. 2010;90:1152–68.

Article  CAS  PubMed  PubMed Central  Google Scholar 

O’Brien AD, Mohawk KL. Mouse models of Escherichia coli O157:H7 Infection and Shiga Toxin Injection. J Biomed Biotechnol. 2011; 2011:258185.

Hopkins EGD, Roumeliotis TI, Mullineaux-Sanders C, Choudhary JS, Frankel G. Intestinal epithelial cells and the microbiome undergo swift reprogramming at the inception of colonic Citrobacter rodentium infection. mBio. 2019;10:1–19.

Article  Google Scholar 

Yadav M, Chauhan NS. Microbiome therapeutics: exploring the present scenario and challenges. Gastroenterol Rep (Oxf). 2022;10:goab046.

Article  PubMed  Google Scholar 

Lynn DJ, Benson SC, Lynn MA, Pulendran B. Modulation of immune responses to vaccination by the microbiota: implications and potential mechanisms. Nat Rev Immunol. 2021;22:33–46.

Article  PubMed  PubMed Central  Google Scholar 

Zimmermann P. The immunological interplay between vaccination and the intestinal microbiota. NPJ Vaccines. 2023;8:24.

Article  PubMed  PubMed Central  Google Scholar 

Hays MP, Ericsson AC, Yang Y, Hardwidge PR. Vaccinating with conserved Escherichia coli antigens does not alter the mouse intestinal microbiome. BMC Res Notes. 2016;9:1–7.

Article  Google Scholar 

Rojas-Lopez M, Monterio R, Pizza M, Desvaux M, Rosini R. Intestinal pathogenic Escherichia coli: insights for vaccine development. Front Microbiol. 2018;9:333650.

Article  Google Scholar 

Sanchez-Villamil JI, Tapia D, Torres AG. Optimization of multivalent gold nanoparticle vaccines eliciting Humoral and Cellular Immunity in an in vivo model of Enterohemorrhagic Escherichia coli O157:H7 colonization. mSphere. 2022;7:e0093421.

Article  PubMed  Google Scholar 

Sanchez-Villamil JI, Tapia D, Torres AG. Development of a gold nanoparticle vaccine against Enterohemorrhagic Escherichia coli O157:H7. mBio. 2019;10:e01869–19.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bowser S, Melton-Celsa A, Chapartegui-González I, Torres AG. Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model. Microbiol Spectr. 2023;12:e02261.

PubMed  PubMed Central  Google Scholar 

Bowser S, Melton-Celsa A, Chapartegui-González I, Torres AG. Further evaluation of Enterohemorrhagic Escherichia coli Gold Nanoparticle vaccines utilizing Citrobacter rodentium as the Model Organism. Vaccines (Basel). 2024;12:508.

Article  CAS  PubMed  Google Scholar 

Gansheroff LJ, Wachtel MR, O’Brien AD. Decreased adherence of enterohemorrhagic Escherichia coli to HEp-2 cells in the presence of antibodies that recognize the C-terminal region of intimin. Infect Immun. 1999;67:6409–17.

Article  CAS  PubMed  PubMed Central  Google Scholar 

John Turkevich B, Cooper Stevenson P, Hillier J. A study of the nucleation and growth processes in the synthesis of Colloidal Gold. Discuss Faraday Soc. 1951;11:55–75.

Article  Google Scholar 

Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 2013;41:e1.

Article  CAS  PubMed  Google Scholar 

Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013;41:D590–596.

Article  CAS  PubMed  Google Scholar 

Chong J, Liu P, Zhou G, Xia J. Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data. Nat Protoc. 2020;15:799–821.

Article  CAS  PubMed  Google Scholar 

Lu Y, Zhou G, Ewald J, Pang Z, Shiri T, Xia J. MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data. Nucleic Acids Res. 2023;51:W310–318.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Elliott SJ, Sperandio V, Giron JA, Shin S, Mellies JL, Wainwright L, et al. The locus of enterocyte effacement (LEE)-encoded regulator controls expression of both LEE- and non-LEE-encoded virulence factors in Enteropathogenic and Enterohemorrhagic Escherichia coli. Infect Immun. 2000;68:6115–26.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mundy R, Macdonald TT, Dougan G, Frankel G, Wiles S. Citrobacter rodentium of mice and man. Cell Microbiol. 2005;7:1697–706.

Article  CAS  PubMed  Google Scholar 

Lee KS, Jeong YJ, Lee MS. Escherichia coli Shiga Toxins and Gut Microinteractionsctions. Toxins (Basel). 2021;13:416.

Article  CAS  PubMed  Google Scholar 

Freedman SB, Xie J, Neufeld MS, Hamilton WL, Hartling L, Tarr PI, et al. Shiga Toxin–Producing Escherichia coli infection, antibiotics, and risk of developing hemolytic uremic syndrome: a Meta-analysis. Clin Infect Dis. 2016;62:1251–8.

Article  PubMed  PubMed Central  Google Scholar 

Wong CS, Jelacic S, Habeen RL, Watkins SL, Tarr PI. The risk of hemolytic-uremic syndrome after Antibiotic Treatment of Escherichia coli O157:H7 infections. N Engl J Med. 2000;342:1930–6.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kakoullis L, Papachristodoulou E, Chra P, Panos G. Shiga toxin-induced haemolytic uraemic syndrome and the role of antibiotics: a global overview. J Infect. 2019;79:75–94.

Article  PubMed  Google Scholar 

Ritchie JM. Animal models of Enterohemorrhagic Escherichia coli infection. Microbiol Spectr. 2014;2:EHEC–0022.

Article  PubMed  Google Scholar 

Walrath T, Dyamenahalli KU, Hulsebus HJ, McCullough RL, Idrovo JP, Boe DM, et al. Age-related changes in intestinal immunity and the microbiome. J Leukoc Biol. 2021;109:1045–61.

Article  CAS  PubMed