Dynamic chromosome association with nuclear organelles in living cells

Ahmad K, Henikoff S (2002) The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. Mol Cell 9:1191–1200

Article  CAS  PubMed  Google Scholar 

Bartolomei MS, Corden JL (1987) Localization of an alpha-amanitin resistance mutation in the gene encoding the largest subunit of mouse RNA polymerase II. Mol Cell Biol 7:586–594

CAS  PubMed  PubMed Central  Google Scholar 

Belmont AS (2001) Visualizing chromosome dynamics with GFP. Trends Cell Biol 11:250–257

Article  CAS  PubMed  Google Scholar 

Belmont AS, Straight AF (1998) In vivo visualization of chromosomes using lac operator-repressor binding. Trends Cell Biol 8:121–124

Article  CAS  PubMed  Google Scholar 

Bystricky K (2015) Chromosome dynamics and folding in eukaryotes: insights from live cell microscopy. FEBS Lett 589:3014–3022

Article  CAS  PubMed  Google Scholar 

Carpenter AE, Belmont AS (2004) Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo. Methods Enzymol 375:366–381

Article  CAS  PubMed  Google Scholar 

Chen D, Belmont AS, Huang S (2004) Upstream binding factor association induces large-scale chromatin decondensation. Proc Natl Acad Sci USA 101:15106–15111

Article  CAS  PubMed  PubMed Central  Google Scholar 

Clark A, Ellem K (1966) A correlated morphological and functional study of the effects of actinomycin D on hela cells. II. The selective effects of low concentrations on the rapidly labeled types of RNA separated by column chromatography. In Annales Medicinae Experimentalis Et Biologiae Fenniae 44:100–108

CAS  Google Scholar 

Clark AM, Love R, Studzinski GP, Ellem KA (1967) A correlated morphological and functional study of the effects of actinomycin D on HeLa cells. I. Effects on the nucleolar and cytoplasmic ribonucleoproteins. Exp Cell Res 45:106–119

Article  CAS  PubMed  Google Scholar 

Cremer T, Cremer C (2001) Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat Rev Genet 2:292–301

Article  CAS  PubMed  Google Scholar 

Cremer T, Cremer M (2010) Chromosome territories. Cold Spring Harb Perspect Biol 2:a003889

Article  PubMed  PubMed Central  Google Scholar 

Cremer T, Cremer M, Hübner B, Strickfaden H, Smeets D, Popken J, Sterr M, Markaki Y, Rippe K, Cremer C (2015) The 4D nucleome: evidence for a dynamic nuclear landscape based on co-aligned active and inactive nuclear compartments. FEBS Lett 589:2931–2943

Article  CAS  PubMed  Google Scholar 

Cvackova Z, Masata M, Stanek D, Fidlerova H, Raska I (2009) Chromatin position in human HepG2 cells: although being non-random, significantly changed in daughter cells. J Struct Biol 165:107–117

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dekker J, Alber F, Aufmkolk S, Beliveau BJ, Bruneau BG, Belmont AS, Bintu L, Boettiger A, Calandrelli R, Disteche CM et al (2023) Spatial and temporal organization of the genome: current state and future aims of the 4D nucleome project. Mol Cell 83:2624–2640

Article  CAS  PubMed  Google Scholar 

Dietzel S, Belmont AS (2001) Reproducible but dynamic positioning of DNA in chromosomes during mitosis. Nat Cell Biol 3:767–770

Article  CAS  PubMed  Google Scholar 

Eykelenboom JK, Gierliński M, Yue Z, Hegarat N, Pollard H, Fukagawa T, Hochegger H, Tanaka TU (2019) Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis. J Cell Biol 218:1531–1552

Article  CAS  PubMed  PubMed Central  Google Scholar 

Finn EH, Misteli T (2019) Molecular basis and biological function of variability in spatial genome organization. Science 365

Frankowski KJ, Wang C, Patnaik S, Schoenen FJ, Southall N, Li D, Teper Y, Sun W, Kandela I, Hu D et al (2018) Metarrestin, a perinucleolar compartment inhibitor, effectively suppresses metastasis. Sci Transl Med 10

Gallego J, Ortiz AR, De Pascual-Teresa B, Gago F (1997) Structure-affinity relationships for the binding of actinomycin D to DNA. J Comput Aided Mol Des 11:114–128

Article  CAS  PubMed  Google Scholar 

Gerlich D, Beaudouin J, Kalbfuss B, Daigle N, Eils R, Ellenberg J (2003) Global chromosome positions are transmitted through mitosis in mammalian cells. Cell 112:751–764

Article  CAS  PubMed  Google Scholar 

Ide S, Imai R, Ochi H, Maeshima K (2020) Transcriptional suppression of ribosomal DNA with phase separation. Sci Adv 6

Inoué S (2013) Video microscopy. Springer, New York

Google Scholar 

Jacob MD, Audas TE, Uniacke J, Trinkle-Mulcahy L, Lee S (2013) Environmental cues induce a long noncoding RNA-dependent remodeling of the nucleolus. Mol Biol Cell 24:2943–2953

Article  CAS  PubMed  PubMed Central  Google Scholar 

Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH et al (2004) From silencing to gene expression: real-time analysis in single cells. Cell 116:683–698

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kedinger C, Gniazdowski M, Mandel JL Jr, Gissinger F, Chambon P (1970) Alpha-amanitin: a specific inhibitor of one of two DNA-pendent RNA polymerase activities from calf thymus. Biochem Biophys Res Commun 38:165–171

Article  CAS  PubMed  Google Scholar 

Khanna N, Hu Y, Belmont Andrew S (2014) HSP70 transgene directed motion to nuclear speckles facilitates heat shock activation. Curr Biol 24:1138–1144

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kimura H, Cook PR (2001) Kinetics of core histones in living human cells: little exchange of H3 and H4 and some rapid exchange of H2B. J Cell Biol 153:1341–1353

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kind J, Pagie L, Ortabozkoyun H, Boyle S, De Vries SS, Janssen H, Amendola M, Nolen LD, Bickmore WA, Van Steensel B (2013) Single-cell dynamics of genome-nuclear lamina interactions. Cell 153:178–192

Article  CAS  PubMed  Google Scholar 

Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH (2011) Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Science 332:475–478

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lindell TJ, Weinberg F, Morris PW, Roeder RG, Rutter WJ (1970) Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science 170:447–449

Article  CAS  PubMed  Google Scholar 

Misteli T (2020) The self-organizing genome: principles of genome architecture and function. Cell 183:28–45

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nguyen VT, Giannoni F, Dubois MF, Seo SJ, Vigneron M, Kedinger C, Bensaude O (1996) In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin. Nucl Acids Res 24:2924–2929

Article  CAS  PubMed  PubMed Central  Google Scholar 

Perry RP (1963) Selective effects of actinomycin D on the intracellular distribution of RNA synthesis in tissue culture cells. Exp Cell Res 29:400–406

Article  CAS 

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