Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach

Bannister AJ, Kouzarides T. Regulation of chromatin by histone modifications. Cell Res. 2011;21(3):381–95.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Millan-Zambrano G, Burton A, Bannister AJ, Schneider R. Histone post-translational modifications - cause and consequence of genome function. Nat Rev Genet. 2022.

Zaib S, Rana N, Khan I. Histone modifications and their role in Epigenetics of Cancer. Curr Med Chem. 2022;29(14):2399–411.

Article  CAS  PubMed  Google Scholar 

Rape M. Ubiquitylation at the crossroads of development and disease. Nat Rev Mol Cell Biol. 2018;19(1):59–70.

Article  CAS  PubMed  Google Scholar 

Mattiroli F, Penengo L. Histone ubiquitination: an Integrative Signaling Platform in Genome Stability. Trends Genet. 2021;37(6):566–81.

Article  CAS  PubMed  Google Scholar 

Tweedie-Cullen RYRJ, Mansuy IM. Comprehensive mapping of post-translational modifications on synaptic, nuclear and histone proteins in the adult mouse brain. J Proteome Res. 2009;8:4966–82.

Article  CAS  PubMed  Google Scholar 

Mashtalir N, Dao HT, Sankar A, Liu HY, Corin AJ, Bagert JD, et al. Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes. Science. 2021;373(6552):306–.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wilson MD, Benlekbir S, Fradet-Turcotte A, Sherker A, Julien JP, McEwan A, et al. The structural basis of modified nucleosome recognition by 53BP1. Nature. 2016;536(7614):100–3.

Article  CAS  PubMed  Google Scholar 

Debelouchina GT, Gerecht K, Muir TW. Ubiquitin utilizes an acidic surface patch to alter chromatin structure. Nat Chem Biol. 2017;13(1):105–10.

Article  CAS  PubMed  Google Scholar 

Vaughan RM, Kupai A, Rothbart SB. Chromatin regulation through Ubiquitin and Ubiquitin-like histone modifications. Trends Biochem Sci. 2021;46(4):258–69.

Article  CAS  PubMed  Google Scholar 

Shechter D, Dormann HL, Allis CD, Hake SB. Extraction, purification and analysis of histones. Nat Protoc. 2007;2(6):1445–57.

Article  CAS  PubMed  Google Scholar 

Schnitzler GR. Isolation of histones and nucleosome cores from mammalian cells. Curr Protoc Mol Biol. 2001;21(12):1–12.

Google Scholar 

Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, et al. Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol. 2004;375:23–44.

Article  CAS  PubMed  Google Scholar 

Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C. Structural basis for histone H2B deubiquitination by the SAGA DUB module. Science. 2016;351(6274):725–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Luger K, Rechsteiner TJ, Richmond TJ. Expression and purification of recombinant histones and nucleosome reconstitution. Methods Mol Biol. 1999;119:1–16.

CAS  PubMed  Google Scholar 

Chu GC, Zhao R, Wu X, Shi J, Li YM. One-Pot synthesis of a Bis-Thio-Acetone Linked Ubiquitinated histones using 1,3-Dibromoacetone. J Org Chem. 2020;85(23):15631–7.

Article  CAS  PubMed  Google Scholar 

Chen JJ, Stermer D, Tanny JC. Decoding histone ubiquitylation. Front Cell Dev Biol. 2022;10:968398.

Article  PubMed  PubMed Central  Google Scholar 

Shim Y, Duan MR, Chen X, Smerdon MJ, Min JH. Polycistronic coexpression and nondenaturing purification of histone octamers. Anal Biochem. 2012;427(2):190–2.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhou Y, Xie Q, Wang H, Sun H. Chemical approaches for the preparation of ubiquitinated proteins via natural linkages. J Pept Sci. 2022;28(3):e3367.

Article  CAS  PubMed  Google Scholar 

Zheng Q, Su Z, Yu Y, Liu L. Recent progress in dissecting ubiquitin signals with chemical biology tools. Curr Opin Chem Biol. 2022;70:102187.

Article  CAS  PubMed  Google Scholar 

Chatterjee C, McGinty RK, Fierz B, Muir TW. Disulfide-directed histone ubiquitylation reveals plasticity in hDot1L activation. Nat Chem Biol. 2010;6(4):267–9.

Article  CAS  PubMed  Google Scholar 

Long L, Furgason M, Yao TT. Generation of nonhydrolyzable ubiquitin-histone mimics. Methods. 2014;70(2–3):134–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen J, Ai Y, Wang J, Haracska L, Zhuang Z. Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis. Nat Chem Biol. 2010;6(4):270–2.

Article  CAS  PubMed  Google Scholar 

Chu GC, Pan M, Li J, Liu S, Zuo C, Tong ZB, et al. Cysteine-aminoethylation-assisted Chemical Ubiquitination of recombinant histones. J Am Chem Soc. 2019;141(8):3654–63.

Article  CAS  PubMed  Google Scholar 

Hu H, Li J, He Q, Qi Y, Liu C. Synthesis of a Ubiquitinated histone mimic bearing a new Thioether linkage. Synlett. 2017;28(15):1939–43.

Article  Google Scholar 

Lewis YE, Abeywardana T, Lin YH, Galesic A, Pratt MR. Synthesis of a bis-thio-acetone (BTA) analogue of the lysine isopeptide bond and its application to investigate the effects of Ubiquitination and SUMOylation on alpha-synuclein aggregation and toxicity. ACS Chem Biol. 2016;11(4):931–42.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Valkevich EM, Guenette RG, Sanchez NA, Chen YC, Ge Y, Strieter ER. Forging isopeptide bonds using thiol-ene chemistry: site-specific coupling of ubiquitin molecules for studying the activity of isopeptidases. J Am Chem Soc. 2012;134(16):6916–9.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ichikawa Y, Connelly CF, Appleboim A, Miller TC, Jacobi H, Abshiru NA et al. A synthetic biology approach to probing nucleosome symmetry. Elife. 2017;6.

Holt MT, David Y, Pollock S, Tang Z, Jeon J, Kim J, et al. Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin. Proc Natl Acad Sci U S A. 2015;112(33):10365–70.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jbara M, Sun H, Kamnesky G, Brik A. Chemical chromatin ubiquitylation. Curr Opin Chem Biol. 2018;45:18–26.

Article  CAS  PubMed  Google Scholar 

Morgan M, Jbara M, Brik A, Wolberger C. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage. Methods Enzymol. 2019;618:1–27.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ai H, Chu GC, Gong Q, Tong ZB, Deng Z, Liu X, et al. Chemical synthesis of Post-translationally modified H2AX reveals redundancy in interplay between Histone Phosphorylation, Ubiquitination, and methylation on the binding of 53BP1 with nucleosomes. J Am Chem Soc. 2022;144(40):18329–37.

Article  CAS  PubMed  Google Scholar 

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