Adli M (2018) The CRISPR tool kit for genome editing and beyond. Nat Commun 9:1911. https://doi.org/10.1038/s41467-018-04252-2
Article ADS CAS PubMed PubMed Central Google Scholar
Alhaji SY, Ngai SC, Abdullah S (2019) Silencing of transgene expression in mammalian cells by DNA methylation and histone modifications in gene therapy perspective. Biotechnol Genet Eng Rev 35:1–25. https://doi.org/10.1080/02648725.2018.1551594
Article CAS PubMed Google Scholar
An CI, Ichihashi Y, Peng J, Sinha NR, Hagiwara N (2016) Transcriptome Dynamics and potential roles of Sox6 in the postnatal heart. PLoS One 11:e0166574. https://doi.org/10.1371/journal.pone.0166574
Article CAS PubMed PubMed Central Google Scholar
Atlasi Y, Stunnenberg HG (2017) The interplay of epigenetic marks during stem cell differentiation and development. Nat Rev Genet 18:643–658. https://doi.org/10.1038/nrg.2017.57
Article CAS PubMed Google Scholar
Balmas E, Sozza F, Bottini S, Ratto ML, Savore G, Becca S, Snijders KE, Bertero A (2023) Manipulating and studying gene function in human pluripotent stem cell models. FEBS Lett. https://doi.org/10.1002/1873-3468.14709
Battulin N, Korablev A, Ryzhkova A, Smirnov A, Kabirova E, Khabarova A, Lagunov T, Serova I, Serov O (2022) The human EF1a promoter does not provide expression of the transgene in mice. Transgenic Res 31:525–535. https://doi.org/10.1007/s11248-022-00319-5
Article CAS PubMed PubMed Central Google Scholar
Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L (2016) Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development 143:4405–4418. https://doi.org/10.1242/dev.138081
Article CAS PubMed PubMed Central Google Scholar
Bertero A, Yiangou L, Brown S, Ortmann D, Pawlowski M, Vallier L (2018) Conditional manipulation of gene function in human cells with optimized inducible shRNA. Curr Protoc Stem Cell Biol 44:5C41–5C448. https://doi.org/10.1002/cpsc.45
Bertero A, Fields PA, Ramani V, Bonora G, Yardimci GG, Reinecke H, Pabon L, Noble WS, Shendure J, Murry CE (2019) Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory. Nat Commun 10:1538. https://doi.org/10.1038/s41467-019-09483-5
Article ADS CAS PubMed PubMed Central Google Scholar
Bhagwan JR, Collins E, Mosqueira D, Bakar M, Johnson BB, Thompson A, Smith JGW, Denning C (2019) Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour. F1000Res 8:1911. https://doi.org/10.12688/f1000research.19894.2
Article CAS PubMed Google Scholar
Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA (2020) Master regulators and cofactors of human neuronal cell fate specification identified by CRISPR Gene Activation screens. Cell Rep 33:108460. https://doi.org/10.1016/j.celrep.2020.108460
Article CAS PubMed PubMed Central Google Scholar
Burridge PW, Holmstrom A, Wu JC (2015) Chemically defined culture and cardiomyocyte differentiation of human pluripotent stem cells. Curr Protoc Hum Genet 87:2131–21315. https://doi.org/10.1002/0471142905.hg2103s87
Cabrera A, Edelstein HI, Glykofrydis F, Love KS, Palacios S, Tycko J, Zhang M, Lensch S, Shields CE, Livingston M, Weiss R, Zhao H, Haynes KA, Morsut L, Chen YY, Khalil AS, Wong WW, Collins JJ, Rosser SJ, Polizzi K, Elowitz MB, Fussenegger M, Hilton IB, Leonard JN, Bintu L, Galloway KE, Deans TL (2022) The sound of silence: transgene silencing in mammalian cell engineering. Cell Syst 13:950–973. https://doi.org/10.1016/j.cels.2022.11.005
Article CAS PubMed Google Scholar
Casas-Mollano JA, Zinselmeier MH, Erickson SE, Smanski MJ (2020) CRISPR-Cas activators for Engineering Gene expression in higher eukaryotes. CRISPR J 3:350–364. https://doi.org/10.1089/crispr.2020.0064
Article CAS PubMed PubMed Central Google Scholar
Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J (2015) Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. PLoS One 10:e0116032. https://doi.org/10.1371/journal.pone.0116032
Article CAS PubMed PubMed Central Google Scholar
Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P.R.I. E S, Lin S, Kiani CD, Guzman DJ, Wiegand D, Ter-Ovanesyan JL, Braff N, Davidsohn BE, Housden N, Perrimon R, Weiss J, Aach JJ, Collins, Church GM (2015) Highly efficient Cas9-mediated transcriptional programming. Nat Methods 12:326–328. https://doi.org/10.1038/nmeth.3312
Article CAS PubMed PubMed Central Google Scholar
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567. https://doi.org/10.1038/nmeth.3871
Article CAS PubMed PubMed Central Google Scholar
Desfarges SCA (2012) Viral integration and consequences on host gene expression. Essential Agents of Life, Viruses, pp 147–175
Ellis J (2005) Silencing and variegation of gammaretrovirus and lentivirus vectors. Hum Gene Ther 16:1241–1246. https://doi.org/10.1089/hum.2005.16.1241
Article CAS PubMed Google Scholar
Fan Y, Sanyal S, Bruzzone R (2018) Breaking bad: how viruses subvert the cell cycle. Front Cell Infect Microbiol 8:396. https://doi.org/10.3389/fcimb.2018.00396
Article CAS PubMed PubMed Central Google Scholar
Fus-Kujawa A, Prus P, Bajdak-Rusinek K, Teper P, Gawron K, Kowalczuk A, Sieron AL (2021) An overview of methods and tools for transfection of eukaryotic cells in vitro. Front Bioeng Biotechnol 9:701031. https://doi.org/10.3389/fbioe.2021.701031
Article PubMed PubMed Central Google Scholar
Guo J, Ma D, Huang R, Ming J, Ye M, Kee K, Xie Z, Na J (2017) An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells. Protein Cell 8:379–393. https://doi.org/10.1007/s13238-016-0360-8
Article CAS PubMed PubMed Central Google Scholar
Gurung IS, Medina-Gomez G, Kis A, Baker M, Velagapudi V, Neogi SG, Campbell M, Rodriguez-Cuenca S, Lelliott C, McFarlane I, Oresic M, Grace AA, Vidal-Puig A, Huang CL (2011) Deletion of the metabolic transcriptional coactivator PGC1beta induces Cardiac Arrhythmia. Cardiovasc Res 92:29–38. https://doi.org/10.1093/cvr/cvr155
Article CAS PubMed PubMed Central Google Scholar
Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE (2020) A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells. Sci Rep 10:635. https://doi.org/10.1038/s41598-020-57500-1
Article ADS CAS PubMed PubMed Central Google Scholar
Herbst F, Ball CR, Tuorto F, Nowrouzi A, Wang W, Zavidij O, Dieter SM, Fessler S, van der Hoeven F, Kloz U, Lyko F, Schmidt M, von Kalle C, Glimm H (2012) Extensive methylation of promoter sequences silences lentiviral transgene expression during stem cell differentiation in vivo. Mol Ther 20:1014–1021. https://doi.org/10.1038/mt.2012.46
Article CAS PubMed PubMed Central Google Scholar
Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C (2018) On the design of CRISPR-based single-cell molecular screens. Nat Methods 15:271–274. https://doi.org/10.1038/nmeth.4604
Article CAS PubMed PubMed Central Google Scholar
Himeda CL, Chen X, Hauschka SD (2011) Design and testing of regulatory cassettes for optimal activity in skeletal and cardiac muscles. Methods Mol Biol 709:3–19. https://doi.org/10.1007/978-1-61737-982-6_1
Article CAS PubMed Google Scholar
Ho SM, Hartley BJ, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani HP, Akbarian S, Brennand KJ (2017) Evaluating synthetic activation and repression of neuropsychiatric-related genes in hiPSC-Derived NPCs, neurons, and astrocytes. Stem Cell Reports 9:615–628. https://doi.org/10.1016/j.stemcr.2017.06.012
Article CAS PubMed PubMed Central Google Scholar
Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, DeKelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R (2009) Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol 27:851–857. https://doi.org/10.1038/nbt.1562
Article CAS PubMed PubMed Central Google Scholar
Irion S, Luche H, Gadue P, Fehling HJ, Kennedy M, Keller G (2007) Identification and targeting of the ROSA26 locus in human embryonic stem cells. Nat Biotechnol 25:1477–1482.
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