Optimization of Laccase Production, and Characterization of Lignin Degradation Products by Fusarium oxysporum JUMAD-053

Igor S. Shiraishi, Renato P. Dorte, Beatriz R Ribeiro, Gabriela C.P.S. Souza, Robert F.H. Dekker, Luciana Furnaleto Maia, Aneli M. Barbosa-Dekker, Dionísio Borsato, Joseph A.M. Evaristo, Geisa P.C. Evaristo, Julliana I. Simionato, Juliana Feijó de Souza Daniel

The objective of this work was to optimize the culture conditions for laccase production in the presence of Kraft lignin by Fusarium oxysporum JUMAD-053, and to evaluate the biodegradation products of lignin. The fungal isolate that presented highest laccase activity had its production optimized by a statistical factorial design 33 in 15 experimental runs. F. oxysporum presented the highest constitutive laccase titer (5.37 U/mL). Statistical factorial design demonstrated a maximum laccase titer of 9.8 U/mL when assayed against ABTS under the conditions optimized: 1.125% (w/v) yeast extract, 0.5% (w/v) Kraft lignin and 10 days of cultivation. The maximum laccase titer when assayed on DMP was 8.4 U/mL, following the conditions optimized: 1.125% yeast extract, 0.25% Kraft lignin and 7 days of cultivation. The analysis of cultures led to identification of metabolites; two being aromatic: 2,6-dimethoxy benzoic acid and sesamin; also, fumonisin and long-chain fatty acids. As a result of the study, the maximum laccase activities of 9.8 and 8.4 U/mL measured from ABTS and DMP substrates, respectively. The search shows new sources of fungal laccase for obtaining new metabolites of biodegradation from Kraft lignin in culture medium.

DOI: http://dx.doi.org/10.17807/orbital.v13i5.1623

Biodegradation; Factorial design; Kraft lignin; Laccase; Mass spectrometry

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