Cell-Free Systems and Their Importance in the Study of Membrane Proteins

Almeida JG, Preto AJ, Koukos PI, Bonvin AMJJ, Moreira IS (2017) Membrane proteins structures: a review on computational modeling tools. Biochim Biophys Acta Biomembr. https://doi.org/10.1016/j.bbamem.2017.07.008

Article  PubMed  Google Scholar 

Anandan A, Vrielink A (2016) Detergents in membrane protein purification and crystallisation. Adv Exp Med Biol 922:13–28. https://doi.org/10.1007/978-3-319-35072-1_2

Article  CAS  PubMed  Google Scholar 

Bagal SK, Brown AD, Cox PJ et al (2013) Ion channels as therapeutic targets: a drug discovery perspective. J Med Chem 56(3):593–624. https://doi.org/10.1021/jm3011433

Article  CAS  PubMed  Google Scholar 

Bayburt TH, Sligar SG (2003) Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers. Protein Sci 11:2476–2481. https://doi.org/10.1110/ps.03267503

Article  CAS  Google Scholar 

Bayburt TH, Sligar SG (2010) Membrane protein assembly into Nanodiscs. FEBS Lett. 584(9):1721

Article  CAS  PubMed  Google Scholar 

Bechlars S, Wüstenhagen DA, Drägert K, Dieckmann R, Strauch E (2013) Kubick S (2013) cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus. Toxicon 15(76):132–142. https://doi.org/10.1016/j.toxicon.2013.09.012

Article  CAS  Google Scholar 

Berrier C, Park KH, Abes S, Bibonne A, Betton JM, Ghazi A (2004) Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry 43(39):12585–12591. https://doi.org/10.1021/bi049049y

Article  CAS  PubMed  Google Scholar 

Blobel G, Dobberstein B (1975) Transfer of proteins across membranes. I. presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 67(3):835–51. https://doi.org/10.1083/jcb.67.3.835

Article  CAS  PubMed  Google Scholar 

Breyton C, Pucci B, Popot JL (2010) Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro. Methods Mol Biol 601:219–245. https://doi.org/10.1007/978-1-60761-344-2_14

Article  CAS  PubMed  Google Scholar 

Brödel AK, Sonnabend A, Roberts LO, Stech M, Wüstenhagen DA, Kubick S (2013) IRES-mediated translation of membrane proteins and glycoproteins in eukaryotic cell-free systems. PLoS ONE 8(12):e82234. https://doi.org/10.1371/journal.pone.0082234

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brödel AK, Sonnabend A, Kubick S (2014) Cell-free protein expression based on extracts from CHO cells. Biotechnol Bioeng 111(1):25–36. https://doi.org/10.1002/bit.25013

Article  CAS  PubMed  Google Scholar 

Buchner E (1897) Alkoholische Gärung ohne Hefezellen. Ber Dtsch Chem Ges 30(1):1110–1113

Article  CAS  Google Scholar 

Buntru M, Vogel S, Spiegel H, Schillberg S (2014) Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system. BMC Biotechnol 14:37. https://doi.org/10.1186/1472-6750-14-37

Article  CAS  PubMed  PubMed Central  Google Scholar 

Buntru M, Vogel S, Stoff K, Spiegel H, Schillberg S (2015) A versatile coupled cell-free transcription-translation system based on tobacco BY-2 cell lysates. Biotechnol Bioeng 12(5):867–878. https://doi.org/10.1002/bit.25502

Article  CAS  Google Scholar 

Cancedda R, Schlesinger MJ (1974) Formation of Sindbis virus capsid protein in mammalian cell-free extracts programmed with viral messenger RNA. Proc Natl Acad Sci USA 71(5):1843–1847

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chiba CH, Knirsch MC, Azzoni AR, Moreira AR, Stephano MA (2021) Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products. Biotechniques 70(2):126–133. https://doi.org/10.2144/btn-2020-0155

Article  CAS  PubMed  Google Scholar 

Chromy BA, Arroyo E, Blanchette CD, Bench G, Benner H, Cappuccio JA, Coleman MA, Henderson PT, Hinz AK, Kuhn EA, Pesavento JB, Segelke BW, Sulchek TA, Tarasow T, Walsworth VL, Hoeprich PD (2007) Different apolipoproteins impact nanolipoprotein particle formation. J Am Chem Soc 129(46):14348–14354. https://doi.org/10.1021/ja074753y

Article  CAS  PubMed  Google Scholar 

Colant N, Melinek B, Frank S, Rosenberg W, Bracewell DG (2021) Escherichia coli-based cell-free protein synthesis for iterative design of tandem-core virus-like particles. Vaccines (Basel) 9(3):193. https://doi.org/10.3390/vaccines9030193

Article  CAS  PubMed  Google Scholar 

Cole SD, Miklos AE, Chiao AC, Sun ZZ, Lux M (2020) Methodologies for preparation of prokaryotic extracts for cell-free expression systems. Synth Syst Biotechnol 5(4):252–267. https://doi.org/10.1016/j.synbio.2020.07.006

Article  PubMed  PubMed Central  Google Scholar 

Cross TA, Murray DT, Watts A (2013) Helical membrane protein conformations and their environment. Eur Biophys J 42(10):731–755. https://doi.org/10.1007/s00249-013-0925-x

Article  CAS  PubMed  Google Scholar 

Cui Y, Chen X, Wang Z, Lu Y (2022) Cell-free pure system: evolution and achievements. Biodes Res. https://doi.org/10.34133/2022/9847014

Article  PubMed  PubMed Central  Google Scholar 

Czerski L, Sanders CR (2000) Functionality of a membrane protein in bicelles. Anal Biochem 284(2):327–333. https://doi.org/10.1006/abio.2000.4720

Article  CAS  PubMed  Google Scholar 

Das BB, Park SH, Opella SJ (2015) Membrane protein structure from rotational diffusion. Biochim Biophys Acta. 1848:229–45

Article  CAS  PubMed  Google Scholar 

Denisov IG, Grinkova YV, Lazarides AA, Sligar SG (2004) Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. J Am Chem Soc 126(11):3477–3487. https://doi.org/10.1021/ja0393574

Article  CAS  PubMed  Google Scholar 

Dürr UH, Gildenberg M, Ramamoorthy A (2012) The magic of bicelles lights up membrane protein structure. Chem Rev. https://doi.org/10.1021/cr300061w

Article  PubMed  PubMed Central  Google Scholar 

Endo Y, Sawasaki T (2006) Cell-free expression systems for eukaryotic protein production. Curr Opin Biotechnol 17(4):373–380. https://doi.org/10.1016/j.copbio.2006.06.009

Article  CAS  PubMed  Google Scholar 

Ezure T, Suzuki T, Higashide S, Shintani E, Endo K, Kobayashi S, Shikata M, Ito M, Tanimizu K, Nishimura O (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol Prog 22(6):1570–1577. https://doi.org/10.1021/bp060110v

Article  CAS  PubMed  Google Scholar 

Ezure T, Nanatani K, Sato Y, Suzuki S, Aizawa K, Souma S, Ito M, Hohsaka T, von Heijine G, Utsumi T, Abe K, Ando E, Uozumi N (2014) A cell-free translocation system using extracts of cultured insect cells to yield functional membrane proteins. PLoS ONE 9(12):e112874. https://doi.org/10.1371/journal.pone.0112874

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ferrer-Miralles N, Domingo-Espín J, Corchero JL, Vázquez E, Villaverde A (2009) Microbial factories for recombinant pharmaceuticals. Microb Cell Fact 8:17. https://doi.org/10.1186/1475-2859-8-17

Article  CAS 

Comments (0)

No login
gif