Abdelrazek NA, Elkhatib WF, Raafat MM, Aboulwafa MM (2020) Production, characterization and bioinformatics analysis of L-asparaginase from a new Stenotrophomonas maltophilia EMCC2297 soil isolate. AMB Express 10(1):1–16

Article  Google Scholar 

Abdulrahman Z (2007) Induction of gene amplification contribute to Sulfonamide Resistance in Proteus mirabilis. J Educ Sci 20(2):116–124

Google Scholar 

Ahmad N, Pandit NP, Maheshwari SK (2012) L-asparaginase gene-a therapeutic approach towards drugs for cancer cell. Int J Biosci 2(4):1–11

Google Scholar 

Alrumman S, Mostafa Y, Al-Izran KA, Alfaifi M, Taha T, Elbehairi S (2019) Production and anticancer activity of an L-asparaginase from Bacillus licheniformis isolated from the Red Sea, Saudi Arabia. Sci Rep 9(1):3756

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ambreen A, Zia MA, Asgher M, Muhammad F (2019) Purification of robust L-Asparaginase from Capsicum annum and its therapeutic efficacy in Leukemia. Pakistan Veterinary J 39(3)

Berridge MV, Herst PM, Tan AS (2005) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annual Rev 11:127–152

Article  CAS  Google Scholar 

Berrouet C, Dorilas N, Rejniak KA, Tuncer N (2020) Comparison of drug inhibitory effects (IC 50) in monolayer and spheroid cultures. Bull Math Biol 82(6):68

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254

Article  CAS  PubMed  Google Scholar 

Bray F, Ferlay J, Soerjomataram I, Siegel R, Torre L, Jemal A (2020) Erratum: global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. Ca Cancer J Clin 70(4):313

Article  Google Scholar 

Cachumba JJM, Antunes FAF, Peres GFD, Brumano LP, Santos JCD, Silva SSD (2016) Current applications and different approaches for microbial l-asparaginase production. Brazilian J Microbiol 47:77–85

Article  CAS  Google Scholar 

Ciesarová Z, Kukurová K (2024) Impact of l-asparaginase on acrylamide content in fried potato and bakery products Acrylamide in Food. Elsevier, pp 473–491

Cruz LS, Kanunfre CC, de Andrade EA, de Oliveira AA, Cruz LS, de Faria Moss M, Sassaki GL, Alencar Menezes LR, Wang M, Khan IA (2020) Enriched terpenes fractions of the latex of euphorbia umbellata promote apoptosis in leukemic cells. Chem Biodivers 17(9):e2000369

Article  CAS  PubMed  Google Scholar 

Da Costa A, De Assis M, Plotkowski M (1999) Comparative analysis of three methods to assess viability of mammalian cells in culture. Biocell: Official J Sociedades Latinoamericanas De Microscopia Electronica et al 23(1):65–72

Google Scholar 

Darnal S, Patial V, Kumar V, Kumar S, Kumar V, Padwad YS, Singh D (2023) Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics. AMB Express 13(1):22

Article  CAS  PubMed  PubMed Central  Google Scholar 

de Freitas Rodrigues J, Philippsen HK, Dolabela MF, Nagamachi CY, Pieczarka JC (2023) The potential of DHA as Cancer Therapy strategies: a narrative review of in vitro cytotoxicity trials. Nutrients 15(8):2006

Article  PubMed  PubMed Central  Google Scholar 

de Prevenção IC (2019) Vigilância. Estimativa 2020-incidência de câncer no Brasil. Rio de

Desai SS, Hungund BS (2018) Submerged fermentation, purification, and characterization of L-asparaginase from Streptomyces sp. isolated from soil. J Appl Biology Biotechnol 6(5):1–3

Google Scholar 

Dobryakova NV, Zhdanov DD, Sokolov NN, Aleksandrova SS, Pokrovskaya MV, Kudryashova EV (2023) Rhodospirillum rubrum L-Asparaginase conjugates with polyamines of Improved Biocatalytic properties as a New Promising Drug for the treatment of Leukemia. Appl Sci 13(5):3373

Article  CAS  Google Scholar 

dos Santos RM, Costa G, Cerávolo IP, Dias-Souza MV (2020) Antibiofilm potential of Psidium guajava and Passiflora edulis pulp extracts against Staphylococcus aureus, cytotoxicity, and interference on the activity of antimicrobial drugs. Future J Pharm Sci 6(1):1–7

Google Scholar 

Eden O, Shaw M, Lilleyman J, Richards S (1990) Non-randomised study comparing toxicity of Escherichia coli and Erwinia asparaginase in children with leukaemia. Med Pediatr Oncol 18(6):497–502

Article  CAS  PubMed  Google Scholar 

El-Naggar NE-A, El-Shweihy NM (2020) Bioprocess development for L-asparaginase production by Streptomyces rochei, purification and in-vitro efficacy against various human carcinoma cell lines. Sci Rep 10(1):7942

Article  CAS  PubMed  PubMed Central  Google Scholar 

El-Naggar NE-A, Deraz SF, Soliman HM, El-Deeb NM, El-Ewasy SM (2016) Purification, characterization, cytotoxicity and anticancer activities of L-asparaginase, anti-colon cancer protein, from the newly isolated alkaliphilic Streptomyces fradiae NEAE-82. Sci Rep 6(1):1–16

Article  Google Scholar 

El-Naggar NE-A, Deraz SF, El-Ewasy SM, Suddek GM (2018) Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces Brollosae NEAE-115. BMC Pharmacol Toxicol 19(1):1–15

Article  Google Scholar 

Elleboudy NS, ElKhatib WF, Aboulwafa MM, Hassouna NA-H (2016) Production and characterization of Phospholipases C from some Bacillus thuringiensis isolates recovered from Egyptian soil. Int J Biotechnol Wellness Industries 5(1):10–24

Article  CAS  Google Scholar 

Ferrari M, Fornasiero MC, Isetta AM (1990) MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 131(2):165–172

Article  CAS  PubMed  Google Scholar 

Ghasemi A, Asad S, Kabiri M, Dabirmanesh B (2017) Cloning and characterization of Halomonas elongata L-asparaginase, a promising chemotherapeutic agent. Appl Microbiol Biotechnol 101(19):7227–7238

Article  CAS  PubMed  Google Scholar 

Ghasemi M, Turnbull T, Sebastian S, Kempson I (2021) The MTT assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. Int J Mol Sci 22(23):12827

Article  CAS  PubMed  PubMed Central  Google Scholar 

Golbabaie A, Nouri H, Moghimi H, Khaleghian A (2020) l-asparaginase production and enhancement by Sarocladium strictum: in vitro evaluation of anti‐cancerous properties. J Appl Microbiol 129(2):356–366

Article  CAS  PubMed  Google Scholar 

Gomha S, Riyadh SM, Mahmmoud EA, Elaasser MM (2015) Synthesis and anticancer activities of thiazoles, 1, 3-thiazines, and thiazolidine using chitosan-grafted-poly (vinylpyridine) as basic catalyst. Heterocycles 91(6):1227–1243

Article  CAS  Google Scholar 

Gurung N, Ray S, Bose S, Rai V (2013) A broader view: microbial enzymes and their relevance in industries, medicine, and beyond. BioMed research international 2013

Hall R, Alasmari A, Mozaffari S, Mahdipoor P, Parang K, Montazeri Aliabadi H (2021) Peptide/lipid-associated nucleic acids (PLANAs) as a multicomponent siRNA delivery system. Mol Pharm 18(3):986–1002

Article  CAS  PubMed  Google Scholar 

Husain I, Sharma A, Kumar S, Malik F (2016) Purification and characterization of glutaminase free asparaginase from Enterobacter cloacae: in-vitro evaluation of cytotoxic potential against human myeloid leukemia HL-60 cells. PLoS ONE 11(2):e0148877

Article  PubMed  PubMed Central  Google Scholar 

Jain R, Zaidi K, Verma Y, Saxena P (2012) L-asparaginase: A promising enzyme for treatment of acute lymphoblastic leukiemia

Jiskoot W, Kijanka G, Randolph TW, Carpenter JF, Koulov AV, Mahler H-C, Joubert MK, Jawa V, Narhi LO (2016) Mouse models for assessing protein immunogenicity: lessons and challenges. J Pharm Sci 105(5):1567–1575

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kumar S, Darnal S, Patial V, Kumar V, Kumar V, Kumar S, Singh D (2022) Molecular cloning, characterization, and in-silico analysis of L-asparaginase from Himalayan Pseudomonas sp. PCH44. 3 Biotech 12(8):162

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685

Mahajan RV, Saran S, Kameswaran K, Kumar V, Saxena R (2012) Efficient production of L-asparaginase from Bacillus licheniformis with low-glutaminase activity: optimization, scale up and acrylamide degradation studies. Bioresour Technol 125:11–16

Article  CAS  PubMed  Google Scholar 

Mahajan RV, Kumar V, Rajendran V, Saran S, Ghosh PC, Saxena RK (2014) Purification and characterization of a novel and robust L-asparaginase having low-glutaminase activity from Bacillus licheniformis: in vitro evaluation of anti-cancerous properties. PLoS ONE 9(6):e99037

Article  PubMed  PubMed Central  Google Scholar 

MAZLUMOĞLU BŞ (2023) Invitro cytotoxicity test methods: MTT and NRU. Int J PharmATA 3(2):50–53

Google Scholar 

McConnell MJ, Hanna PC, Imperiale MJ (2006) Cytokine response and survival of mice immunized with an adenovirus expressing Bacillus anthracis protective antigen domain 4. Infect Immun 74(2):1009–1015

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63

Article  CAS  PubMed  Google Scholar 

Mostafa Y, Alrumman S, Alamri S, Hashem M, Al-izran K, Alfaifi M, Elbehairi SE, Taha T (2019) Enhanced production of glutaminase-free L-asparaginase by marine Bacillus velezensis and cytotoxic activity against breast cancer cell lines. Electron J Biotechnol 42:6–15

Article  CAS  Google Scholar 

Mukherjee P (2019) Bioassay-guided isolation and evaluation of herbal drugs. Quality Control and Evaluation of Herbal Drugs. Elsevier, Amsterdam, The Netherlands, pp 515–537

Book  Google Scholar 

Niles AL, Moravec RA, Hesselberth PE, Scurria MA, Daily WJ, Riss TL (2007) A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal Biochem 366(2):197–206

Article  CAS  PubMed  Google Scholar 

Norgard MV, Emigholz K, Monahan JJ (1979) Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside. J Bacteriol 138(1):270–272

Article  CAS  PubMed