Zhang X, Zheng G, Ma X, Yang Y, Li G, Rao Q, Nie K, Wu K (2004) Expression of P2X7 in human hematopoietic cell lines and leukemia patients. Leuk Res 28:1313–1322. https://doi.org/10.1016/j.leukres.2004.04.001

Article  CAS  PubMed  Google Scholar 

Bahari G, Tabasi F, Hashemi M, Zakeri Z, Taheri M (2021) Association of P2X7 receptor genetic polymorphisms and expression with rheumatoid arthritis susceptibility in a sample of the Iranian population: a case-control study. Clin Rheumatol 40:3115–3126. https://doi.org/10.1007/s10067-021-05645-3

Article  PubMed  Google Scholar 

Liu X, Zhao Z, Ji R, Zhu J, Sui QQ, Knight GE, Burnstock G, He C, Yuan H, Xiang Z (2017) Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats. Purinergic Signal 13:529–544. https://doi.org/10.1007/s11302-017-9579-y

Article  CAS  PubMed  PubMed Central  Google Scholar 

Martin E, Amar M, Dalle C, Youssef I, Boucher C, Le Duigou C, Brückner M, Prigent A, Sazdovitch V, Halle A, Kanellopoulos JM, Fontaine B, Delatour B, Delarasse C (2019) New role of P2X7 receptor in an Alzheimer’s disease mouse model. Mol Psychiatry 24:108–125. https://doi.org/10.1038/s41380-018-0108-3

Article  CAS  PubMed  Google Scholar 

Fryatt AG, Dayl S, Stavrou A, Schmid R, Evans RJ (2019) Organization of ATP-gated P2X1 receptor intracellular termini in apo and desensitized states. J Gen Physiol 151:146–155. https://doi.org/10.1085/jgp.201812108

Article  CAS  PubMed  PubMed Central  Google Scholar 

McCarthy AE, Yoshioka C, Mansoor SE (2019) Full-length P2X7 structures reveal how palmitoylation prevents channel desensitization. Cell 179:659–670e613. https://doi.org/10.1016/j.cell.2019.09.017

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pegoraro A, De Marchi E, Adinolfi E (2021) P2X7 variants in oncogenesis. Cells 10:189. https://doi.org/10.3390/cells10010189

Article  CAS  PubMed  PubMed Central  Google Scholar 

Adinolfi E, Callegari MG, Ferrari D, Bolognesi C, Minelli M, Wieckowski MR, Pinton P, Rizzuto R, Di Virgilio F (2005) Basal activation of the P2X7 ATP receptor elevates mitochondrial calcium and potential, increases cellular ATP levels, and promotes serum-independent growth. Mol Biol Cell 16:3260–3272. https://doi.org/10.1091/mbc.e04-11-1025

Article  CAS  PubMed  PubMed Central  Google Scholar 

Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, Kroemer G, Ishibashi T (2011) Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am J Pathol 179:2798–2809. https://doi.org/10.1016/j.ajpath.2011.08.035

Article  CAS  PubMed  PubMed Central  Google Scholar 

Virginio C, MacKenzie A, North RA, Surprenant A (1999) Kinetics of cell lysis, dye uptake and permeability changes in cells expressing the rat P2X7 receptor. J Physiol 519:335–346. https://doi.org/10.1111/j.1469-7793.1999.0335m.x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Riedel T, Schmalzing G, Markwardt F (2007) Influence of extracellular monovalent cations on pore and gating properties of P2X7 receptor-operated single-channel currents. Biophys J 93:846–858. https://doi.org/10.1529/biophysj.106.103614

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li M, Toombes GE, Silberberg SD, Swartz KJ (2015) Physical basis of apparent pore dilation of ATP-activated P2X receptor channels. Nat Neurosci 18:1577–1583. https://doi.org/10.1038/nn.4120

Article  CAS  PubMed  PubMed Central  Google Scholar 

Illes P, Müller CE, Jacobson KA, Grutter T, Nicke A, Fountain SJ, Kennedy C, Schmalzing G, Jarvis MF, Stojilkovic SS, King BF, Di Virgilio F (2021) Update of P2X receptor properties and their pharmacology: IUPHAR review 30. Br J Pharmacol 178:489–514. https://doi.org/10.1111/bph.15299

Article  CAS  PubMed  Google Scholar 

Keystone EC, Wang MM, Layton M, Hollis S, McInnes IB (2012) Clinical evaluation of the efficacy of the P2X7 purinergic receptor antagonist AZD9056 on the signs and symptoms of rheumatoid arthritis in patients with active disease despite treatment with methotrexate or sulphasalazine. Ann Rheum Dis 71:1630–1635. https://doi.org/10.1136/annrheumdis-2011-143578

Article  CAS  PubMed  Google Scholar 

Stock TC, Bloom BJ, Wei N, Ishaq S, Park W, Wang X, Gupta P, Mebus CA (2012) Efficacy and safety of CE-224,535, an antagonist of P2X7 receptor, in treatment of patients with rheumatoid arthritis inadequately controlled by methotrexate. J Rheumatol 39:720–727. https://doi.org/10.3899/jrheum.110874

Article  CAS  PubMed  Google Scholar 

Cheewatrakoolpong B, Gilchrest H, Anthes JC, Greenfeder S (2005) Identification and characterization of splice variants of the human P2X7 ATP channel. Biochem Biophys Res Commun 332:17–27. https://doi.org/10.1016/j.bbrc.2005.04.087

Article  CAS  PubMed  Google Scholar 

Feng YH, Li X, Wang L, Zhou L, Gorodeski GI (2006) A truncated P2X7 receptor variant (P2X7-j) endogenously expressed in cervical cancer cells antagonizes the full-length P2X7 receptor through hetero-oligomerization. J Biol Chem 281:17228–17237. https://doi.org/10.1074/jbc.M602999200

Article  CAS  PubMed  Google Scholar 

Skarratt KK, Gu BJ, Lovelace MD, Milligan CJ, Stokes L, Glover R, Petrou S, Wiley JS, Fuller SJ (2020) A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function. FASEB J 34:3884–3901. https://doi.org/10.1096/fj.201901198RR

Article  CAS  PubMed  Google Scholar 

Papadopoulos JS, Agarwala R (2007) COBALT: constraint-based alignment tool for multiple protein sequences. Bioinformatics 23:1073–1079. https://doi.org/10.1093/bioinformatics/btm076

Article  CAS  PubMed  Google Scholar 

Adinolfi E, Cirillo M, Woltersdorf R, Falzoni S, Chiozzi P, Pellegatti P, Callegari MG, Sandonà D, Markwardt F, Schmalzing G, Di Virgilio F (2010) Trophic activity of a naturally occurring truncated isoform of the P2X7 receptor. FASEB J 24:3393–3404. https://doi.org/10.1096/fj.09-153601

Article  CAS  PubMed  Google Scholar 

Rassendren F, Buell GN, Virginio C, Collo G, North RA, Surprenant A (1997) The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA. J Biol Chem 272:5482–5486. https://doi.org/10.1074/jbc.272.9.5482

Article  CAS  PubMed  Google Scholar 

Pegoraro A, Orioli E, De Marchi E, Salvestrini V, Milani A, Di Virgilio F, Curti A, Adinolfi E (2020) Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression. Cell Death Dis 11:876. https://doi.org/10.1038/s41419-020-03058-9

Article  CAS  PubMed  PubMed Central  Google Scholar 

Giuliani AL, Colognesi D, Ricco T, Roncato C, Capece M, Amoroso F, Wang QG, De Marchi E, Gartland A, Di Virgilio F, Adinolfi E (2014) Trophic activity of human P2X7 receptor isoforms A and B in osteosarcoma. PLoS ONE 9:e107224. https://doi.org/10.1371/journal.pone.0107224

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ulrich H, Ratajczak MZ, Schneider G, Adinolfi E, Orioli E, Ferrazoli EG, Glaser T, Corrêa-Velloso J, Martins PCM, Coutinho F, Santos APJ, Pillat MM, Sack U, Lameu C (2018) Kinin and purine signaling contributes to neuroblastoma Metastasis. Front Pharmacol 9. https://doi.org/10.3389/fphar.2018.00500

De Salis SKF, Li L, Chen Z, Lam KW, Skarratt KK, Balle T, Fuller SJ (2022) Alternatively spliced isoforms of the P2X7 receptor: structure, function and disease associations. Int J Mol Sci 15:8174. https://doi.org/10.3390/ijms23158174

Article  CAS  Google Scholar 

Karasawa A, Kawate T (2016) Structural basis for subtype-specific inhibition of the P2X7 receptor. eLife 5:e22153. https://doi.org/10.7554/eLife.22153

Article  PubMed  PubMed Central  Google Scholar 

Evans R, O’Neill M, Pritzel A, Antropova N, Senior A, Green T, Žídek A, Bates R, Blackwell S, Yim J, Ronneberger O, Bodenstein S, Zielinski M, Bridgland A, Potapenko A, Cowie A, Tunyasuvunakool K, Jain R, Clancy E, Kohli P, Jumper J, Hassabis D (2022) Protein complex prediction with AlphaFold-Multimer. bioRxiv. https://doi.org/10.1101/2021.10.04.463034

Article  PubMed  PubMed Central  Google Scholar 

Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, Tunyasuvunakool K, Bates R, Žídek A, Potapenko A, Bridgland A, Meyer C, Kohl SAA, Ballard AJ, Cowie A, Romera-Paredes B, Nikolov S, Jain R, Adler J, Back T, Petersen S, Reiman D, Clancy E, Zielinski M, Steinegger M, Pacholska M, Berghammer T, Bodenstein S, Silver D, Vinyals O, Senior AW, Kavukcuoglu K, Kohli P, Hassabis D (2021) Highly accurate protein structure prediction with AlphaFold. Nature 596:583–589. https://doi.org/10.1038/s41586-021-03819-2

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mirdita M, Schütze K, Moriwaki Y, Heo L, Ovchinnikov S, Steinegger M (2022) ColabFold: making protein folding accessible to all. Nat Methods 19:679–682. https://doi.org/10.1038/s41592-022-01488-1

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hilbert M, Böhm G, Jaenicke R (1993) Structural relationships of homologous proteins as a fundamental principle in homology modeling. Proteins 17:138–151. https://doi.org/10.1002/prot.340170204

Article  CAS  PubMed  Google Scholar 

Kryshtafovych A, Moult J, Albrecht R, Chang GA, Chao K, Fraser A, Greenfield J, Hartmann MD, Herzberg O, Josts I, Leiman PG, Linden SB, Lupas AN, Nelson DC, Rees SD, Shang X, Sokolova ML, Tidow H (2021) Computational mode