Barsh GS, Schwartz MW. Genetic approaches to studying energy balance: perception and integration. Nat Rev Genet. 2002;3:589–600.

Article  CAS  PubMed  Google Scholar 

Bouret SG, Draper SJ, Simerly RB. Trophic action of leptin on hypothalamic neurons that regulate feeding. Science. 2004;304:108–10.

Article  CAS  PubMed  Google Scholar 

van der Klaauw AA, Croizier S, Mendes de Oliveira E, Stadler LKJ, Park S, Kong Y, et al. Human semaphorin 3 variants link melanocortin circuit development and energy balance. Cell. 2019;176:729–42.e718.

Article  PubMed  PubMed Central  Google Scholar 

Vogt MC, Paeger L, Hess S, Steculorum SM, Awazawa M, Hampel B, et al. Neonatal insulin action impairs hypothalamic neurocircuit formation in response to maternal high-fat feeding. Cell. 2014;156:495–509.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Schafer DP, Lehrman EK, Kautzman AG, Koyama R, Mardinly AR, Yamasaki R, et al. Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner. Neuron. 2012;74:691–705.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Miyamoto A, Wake H, Ishikawa AW, Eto K, Shibata K, Murakoshi H, et al. Microglia contact induces synapse formation in developing somatosensory cortex. Nat Commun. 2016;7:12540.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Paolicelli RC, Bolasco G, Pagani F, Maggi L, Scianni M, Panzanelli P, et al. Synaptic pruning by microglia is necessary for normal brain development. Science. 2011;333:1456–8.

Article  CAS  PubMed  Google Scholar 

Zhan Y, Paolicelli RC, Sforazzini F, Weinhard L, Bolasco G, Pagani F, et al. Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior. Nat Neurosci. 2014;17:400–6.

Article  CAS  PubMed  Google Scholar 

Malkova NV, Yu CZ, Hsiao EY, Moore MJ, Patterson PH. Maternal immune activation yields offspring displaying mouse versions of the three core symptoms of autism. Brain, Behav, Immun. 2012;26:607–16.

Article  CAS  PubMed  Google Scholar 

Thaler JP, Yi CX, Schur EA, Guyenet SJ, Hwang BH, Dietrich MO, et al. Obesity is associated with hypothalamic injury in rodents and humans. J Clin Invest. 2012;122:153–62.

Article  CAS  PubMed  Google Scholar 

Valdearcos M, Douglass JD, Robblee MM, Dorfman MD, Stifler DR, Bennett ML, et al. Microglial inflammatory signaling orchestrates the hypothalamic immune response to dietary excess and mediates obesity susceptibility. Cell Metab. 2017;26:185–97.e183.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gao Y, Ottaway N, Schriever SC, Legutko B, García-Cáceres C, de la Fuente E, et al. Hormones and diet, but not body weight, control hypothalamic microglial activity. Glia. 2014;62:17–25.

Article  PubMed  Google Scholar 

Gao Y, Bielohuby M, Fleming T, Grabner GF, Foppen E, Bernhard W, et al. Dietary sugars, not lipids, drive hypothalamic inflammation. Mol Metab. 2017;6:897–908.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rosin JM, Vora SR, Kurrasch DM. Depletion of embryonic microglia using the CSF1R inhibitor PLX5622 has adverse sex-specific effects on mice, including accelerated weight gain, hyperactivity and anxiolytic-like behaviour. Brain Behav Immun. 2018;73:682–97.

Article  CAS  PubMed  Google Scholar 

Rosin JM, Marsters CM, Malik F, Far R, Adnani L, Schuurmans C, et al. Embryonic microglia interact with hypothalamic radial glia during development and upregulate the TAM receptors MERTK and AXL following an Insult. Cell Rep. 2021;34:108587.

Article  CAS  PubMed  Google Scholar 

Mirzadeh Z, Alonge KM, Cabrales E, Herranz-Pérez V, Scarlett JM, Brown JM, et al. Perineuronal net formation during the critical period for neuronal maturation in the hypothalamic arcuate nucleus. Nat Metab. 2019;1:212–21.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kawasaki H, Kosugi I, Sakao-Suzuki M, Meguro S, Tsutsui Y, Iwashita T. Intracerebroventricular and intravascular injection of viral particles and fluorescent microbeads into the neonatal brain. J Vis Exp. 2016;24:54164.

Hagemeyer N, Hanft KM, Akriditou MA, Unger N, Park ES, Stanley ER, et al. Microglia contribute to normal myelinogenesis and to oligodendrocyte progenitor maintenance during adulthood. Acta Neuropathol. 2017;134:441–58.

Article  PubMed  PubMed Central  Google Scholar 

Eyo UB, Miner SA, Weiner JA, Dailey ME. Developmental changes in microglial mobilization are independent of apoptosis in the neonatal mouse hippocampus. Brain Behav Immun. 2016;55:49–59.

Article  CAS  PubMed  Google Scholar 

Scott-Hewitt N, Perrucci F, Morini R, Erreni M, Mahoney M, Witkowska A, et al. Local externalization of phosphatidylserine mediates developmental synaptic pruning by microglia. Embo J. 2020;39:e105380.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Baquero AF, de Solis AJ, Lindsley SR, Kirigiti MA, Smith MS, Cowley MA, et al. Developmental switch of leptin signaling in arcuate nucleus neurons. J Neurosci. 2014;34:9982–94.

Article  PubMed  PubMed Central  Google Scholar 

Zimmer MR, Fonseca AHO, Iyilikci O, Pra RD, Dietrich MO. Functional ontogeny of hypothalamic agrp neurons in neonatal mouse behaviors. Cell. 2019;178:44–59.e47.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu YJ, Spangenberg EE, Tang B, Holmes TC, Green KN, Xu X. Microglia elimination increases neural circuit connectivity and activity in adult mouse cortex. J Neurosci. 2021;41:1274–87.

Article  CAS  PubMed  PubMed Central  Google Scholar 

VanRyzin JW, Yu SJ, Perez-Pouchoulen M, McCarthy MM. Temporary depletion of microglia during the early postnatal period induces lasting sex-dependent and sex-independent effects on behavior in rats. eNeuro. 2016;3:ENEURO.0297-16.2016.

Article  PubMed  PubMed Central  Google Scholar 

Romanov RA, Tretiakov EO, Kastriti ME, Zupancic M, Häring M, Korchynska S, et al. Molecular design of hypothalamus development. Nature. 2020;582:246–52.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bouret SG, Draper SJ, Simerly RB. Formation of projection pathways from the arcuate nucleus of the hypothalamus to hypothalamic regions implicated in the neural control of feeding behavior in mice. J Neurosci. 2004;24:2797–805.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kohnke S, Buller S, Nuzzaci D, Ridley K, Lam B, Pivonkova H, et al. Nutritional regulation of oligodendrocyte differentiation regulates perineuronal net remodeling in the median eminence. Cell Rep. 2021;36:109362.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pizzorusso T, Medini P, Berardi N, Chierzi S, Fawcett JW, Maffei L. Reactivation of ocular dominance plasticity in the adult visual cortex. Science. 2002;298:1248–51.

Article  CAS  PubMed  Google Scholar 

Carulli D, Pizzorusso T, Kwok JC, Putignano E, Poli A, Forostyak S, et al. Animals lacking link protein have attenuated perineuronal nets and persistent plasticity. Brain. 2010;133:2331–47.

Article  PubMed  Google Scholar 

Balmer TS, Carels VM, Frisch JL, Nick TA. Modulation of perineuronal nets and parvalbumin with developmental song learning. J Neurosci. 2009;29:12878–85.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nowicka D, Soulsby S, Skangiel-Kramska J, Glazewski S. Parvalbumin-containing neurons, perineuronal nets and experience-dependent plasticity in murine barrel cortex. Eur J Neurosci. 2009;30:2053–63.

Article  PubMed  Google Scholar 

Crapser JD, Arreola MA, Tsourmas KI, Green KN. Microglia as hackers of the matrix: sculpting synapses and the extracellular space. Cell Mol Immunol. 2021;18:2472–88.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nguyen PT, Dorman LC, Pan S, Vainchtein ID, Han RT, Nakao-Inoue H, et al. Microglial remodeling of the extracellular matrix promotes synapse plasticity. Cell. 2020;182:388–403.e315.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pirbhoy PS, Rais M, Lovelace JW, Woodard W, Razak KA, Binder DK, et al. Acute pharmacological inhibition of matrix metalloproteinase-9 activity during development restores perineuronal net formation and normalizes auditory processing in Fmr1 KO mice. J Neurochem. 2020;155:538–58.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bilousova TV, Dansie L, Ngo M, Aye J, Charles JR, Ethell DW, et al. Minocycline promotes dendritic spine maturation and improves behavioural performance in the fragile X mouse model. J Med Genet. 2009;46:94–102.

Article  CAS