The 10 studies included determined the number of asbestos fibers in specific tissues (larynx, myocardium and thoracic lymph nodes) on 52 subjects, from whom 108 samples of tissue were extracted. No article met the inclusion criteria of the scoping review for pharynx and trachea.

The samples were mostly obtained from autopsies and only one study analyzed samples from biopsy. Few data for each type of tissue were obtained from the analysis of all included articles, not allowing a comparison of asbestos fibers found in specific tissues in relation to different population groups (i.e., cases with asbestos-related diagnoses compared to general population).

A description of the subjects and conditions included in the studies was reported in Table 1.

As shown in Table 2, in most of the studies included in this review the analysis and quantification of asbestos fibers was carried out using TEM with EDX. This technology may detect both short (< 5 µm) and longer/thin fibers [9]. However, the counting schemes chosen by the authors are different. For example, Gordon et al. [16] counted only long fibers (> 5 µm) although, as stated by the authors themselves, they observed even the smallest ones using TEM.

Thus, the comparison of asbestos fibers concentrations obtained from different studies over time, is difficult and limits information on the number, type and size of fibers detected.

As reported in Table 3, a quantitative assessment of asbestos fibers of the lymph nodes was analyzed in 9 studies from 1979 to 2014 [7,8,9,10,11, 16, 21, 29] which included 48 subjects. The cases were mostly men (40 subjects) from 15 to 78 years old, while women represented a smaller sample (8 subjects) with ages from 12 to 82 years old.

The total of the samples analyzed was 102, of which 88 had asbestos fibers (mostly amphibole types). At least one type of asbestos fibers (amphiboles or chrysotile) was found in each of the samples taken from cases with occupational exposure, as might be expected. However, the data show the presence of asbestos fibers even in most of those not occupationally exposed, highlighting the theme of environmental exposure or the use of products contaminated or containing asbestos fibers [8, 9, 16].

From the range of samples with asbestos fibers, it is not possible to deduce a uniform pattern of distribution, with extreme values that are 0.003 mfgdt and 7400 mfgdt (a particularly high value found in an asbestos sprayer).

Most studies observed fibers shorter than 5 µm (average lengths observed in different studies were in the range from 1.27 µm to 4.12 µm).

Hirsch et al. [21] analyzed one sample of mediastinal lymph node from a case of a 51-year-old man (smoker) who had an occupational history in a welding factory. In the same case, he previously took a biopsy sample of a vocal polyp in which asbestos fibers were found (see below). It was found by TEM with energy dispersive X-ray spectroscopy (EDS) that the amphibole type was predominant (concentration of 6.4 mfgdt). The detection limit was 0.5 mfgdt for asbestos fibers. The mean length of the fibers detected was 3.3 µm, with a mean diameter of 0.15 µm.

Roggli and Benning [29] studied histologic sections of hilar lymph nodes from 3 autopsies. The samples were taken from men who had an occupational history of exposure to asbestos and who had been diagnosed with asbestosis. The observation of asbestos fibers was performed by SEM, with a detection limit of 0.1 mgfdt. The range was from 0.1 mfgdt to 9.01 mfgdt and included only fibers with a length of 5 µm or greater. No other information about dimension or diameter was reported.

Dodson et al. [7] performed quantification of asbestos fibers in tracheal lymph nodes samples from 8 subjects who were occupationally exposed. Medical histories of the shipyard workers showed several diseases such asbestosis, lung cancer, lung fibrosis, emphysema and cardiovascular pathologies. Subjects were 58 to 82 years of age at the time of autopsy. The analysis was conducted by TEM with energy dispersive X-ray analysis (EDX) and the detection limit was 0.003 mfgdt. Asbestos fibers were defined as fibers with length >  = 0.5 µm and sides roughly parallel to the fiber axis, conforming to a 3:1 aspect ratio. In most of the subjects the numbers of asbestos fibers per gram of dry tissue from the tracheal lymph nodes exceeded the numbers per gram in the lung (belonging to the same cases). Amphibole fibers were detected in all lymph node samples (with a concentration ranging from 0.003 mfgdt to 1.7 mfgdt). The average length of amphibole fibers was 2.22 ± 3.36 µm and the average width was 0.21 ± 0.12 µm. Chrysotile fibers were identified in 6/8 samples (concentration ranging from not detected to 0.018 mfgdt) and 2/8 did not present chrysotile fibers. The average length of chrysotile fibers was 1.27 ± 1.07 µm and the average width was 0.08 ± 0.06 µm.

Tossavainen et al. [34] analyzed tissue of hilar lymph node from an autopsy of a worker who had died from lung cancer and was employed as crocidolite sprayer. Other samples of tissues were taken from the same case. The sample was observed by SEM with a detection limit of 0.1 mfgdt. Lymph node tissue had the highest asbestos fiber level (7400 mfgdt), followed by lung parenchyma, visceral pleura and parietal pleura. Asbestos fibers > 1 µm in length and > 0.3 µm in diameter were counted. The median dimensions of crocidolite fibers were 2.0 to 3.0 µm for length and 0.12 to 0.13 µm for diameter with no obvious difference between various tissues. Size of crocidolite fibers in hilar lymph nodes had a length measure of 1 to 6.0 µm with a median of 2.2 µm and a diameter measure of 0.05 to 0.30 µm with a median of 0.13 µm. For comparison, asbestos fibers were also counted from lung parenchyma, lymph nodes, and kidney cortex in ten autopsied control cases collected from an unselected series of sudden Finnish male deaths. The concentration in the lymph nodes of the controls was in the range from 5 to 81 mfgdt with a mean value of 21 mfgdt. The data of these control cases were not reported in the tables since information was not available in the article.

In 2000, Dodson et al. [8] determined the concentrations of asbestos fibers in the lymph nodes of the general population. Samples of peritracheal lymph nodes and lung tissue from 21 individuals non-occupationally exposed to asbestos were taken at autopsy and were analyzed by analytical transmission electron microscopy (ATEM) and EDX. The detection limit was 0.11 mgfdt. Amphiboles fibers were detected in 13/8 samples with a range of 0.11 mgfdt to 1.7 mgfdt. The most common type of asbestos found in lymph nodes was anthophyllite, followed by tremolite and amosite. No chrysotile fibers were detected in the tissue analyzed. All fibers with length 0.5 µm or more and a 3:1 aspect ratio were detected. The mean length for asbestos fibers was 2.52 µm and the mean width was 0.27 µm.

Dodson et Hammar [9] described a case of asbestos burden in hilar lymph node tissue in a 67-yr-old woman who died from mesothelioma and whose only known exposure to asbestos was from smoking crocidolite asbestos-containing filtered cigarettes. From 5 original samples, after the preparation procedures applied on the tissue, the counting of asbestos fibers was made on a unique sample of 0.0094 g of dry tissue. The lymph nodes contained 14.32 mgfdt. The detection limit for asbestos fibers was 0.65 mgfdt and only amphibole fibers were detected (crocidolite, tremolite, anthophyllite). Asbestos fibers < 5 µm in length or longer were counted. The length of the asbestos fibers was from less than 4 µm to 12 µm.

Dodson et al. selected tissue samples from 11 males with a history of asbestos exposure [10]. Their cause of death was mostly from lung cancer and mesothelioma and, in one case, a left paranasal sinus squamous cell carcinoma. The study evaluated concentration of asbestos fibers in lymph nodes taken from locations anatomically defined according to the Naruke lymph node map [27]. All fibers equal to or greater than 0.5 µm in length were analyzed with ATEM equipped with EDX and selected area electron diffraction (SAED). Amphiboles were detected in 59/6 samples, ranging from 0 to 262.25 mgfdt. Asbestos fibers burden of the various lymph nodes from each case showed a preponderance of amphiboles, particularly amosite and crocidolite. The second most commonly encountered asbestos type was tremolite and anthophyllite. Chrysotile fibers were detected in 3/62 samples with a range of 0 to 1.8 mgfdt. The authors reported as a geometric means of total asbestos fibers a value of 3.52 µm in length and a value of 0.15 µm in width.

Dodson et al. [11] presented a case study reported on tissue burden of fibrous dust in an 81-year-old woman with diffuse malignant mesothelioma. Her home was built and renovated with Libby vermiculite. Libby vermiculite is a material with possible contamination of asbestos. The latency period from time of exposure was over 50 years. Three pieces of peribronchial lymph node were taken from autopsy, but only one sample was subjected to laboratory procedure for specific analysis in ATEM. The detection limit matched the lower value reported of 0.5 mfgdt. The data from the high magnification scan of the lymph node preparation were found to contain 1 tremolite asbestos fiber, 1 anthophyllite asbestos fiber, and 6 Libby amphiboles. The 6 Libby amphiboles were equivalent to 0.5 mfgdt. The identification of the only fibers with length > 3 µm was conducted in the area scanned. Libby amphiboles found in all the samples analyzed (lung and lymph node) showed a length from 5 to 68 µm with an average length of 20.7 µm.

Gordon et al. [16] analyzed lymph nodes from a woman with pleural mesothelioma and with no other known exposure to asbestos other than her use of a cosmetic talcum powder. The brand of talcum powder in question contained asbestos, and the application of its talcum powder released inhalable asbestos fibers. It should be remembered that the talcum powder itself it is not always contaminated, however it may contain asbestos impurities [30]. The location of the lymph nodes was not specified, but it can be deduced a thoracic allocation as it is reported in the study that lung and lymph node tissue were removed at autopsy. TEM analysis of the tissue revealed amphibole asbestos fibers with a value of 0.127 mfgdt, with a limit of detection of 0.021 mfgdt. The amphiboles were identified by EDS and SAED analysis as anthophyllite and tremolite and they were seen in a ratio of 5:1 anthophyllite: tremolite. Many fibers less than 5 µm in length were not counted, only fibers 5 µm or more in length and with aspect ratios of 10:1 or greater.

A quantitative assessment of asbestos fibers of the larynx was analyzed in 2 studies [21, 28] as reported in Table 4.

In the study of Hirsch et al. [21] one sample was taken from a biopsy of a case with vocal polyp and one sample from a case with laryngeal carcinoma. Asbestos fibers were detected in the 2 samples deriving from the 2 patients, no case was found without asbestos fibers. The range of asbestos fibers was from 0.5 mfgdt to 3.6 mfgdt. Both patients were occupationally exposed to asbestos. Both chrysotile and amphibole fibers were found. In one case, mostly chrysotile type fibers were found, in the other case mostly amphiboles. The mean length of the fibers detected ranged from 2.4 µm to 3.4 µm, with a mean diameter from 0.08 µm to 0.14 µm.

In 1997, Pollice et al. [28] reported the concentration of asbestos fibers in 7 samples of extra-abdominal tissues from 3 cases. Two cases had pulmonary asbestosis with a history of asbestos exposure and one control case died for other cause without asbestos exposure. Autoptic samples of the three men were analysed by TEM and energy dispersion spectrometry (EDAX). The analytical procedures described in the study do not specify the detection limit, but the minimum concentration detected was 0.1 mfgdt. In both cases and in the control case, the fibers in larynx tissues were absent or not detectable.

Myocardium has been only analyzed in the article by [28], as shown in Table 5. Three samples were taken from 3 autopsies, one for each case. No asbestos fibers were found in the histological sections of the three reported cases [28], although fibers were counted in the lungs and pleura of the occupationally exposed cases.