Site-specific antibody masking enables conditional activation with different stimuli

AbbreviationsAPMA

4-Aminophenylmercuric acetate

CDR

complementary determining region

DIC

N,N-Diisopropylcarbodiimide

Fmoc

fluorenylmethoxycarbonyl

FPLC

fast protein liquid chromatography

IMAC

immobilised metal chelate ion affinity chromatography

LC-MS

liquid chromatography-mass spectrometry

MMP

matrix metalloproteinase

QTOF

quadrupole time of flight

scFv

single-chain variable fragment

SEC

size exclusion chromatography

TCEP

Tris(2-carboxyethyl)phosphine

UHPLC

Ultra-high-performance liquid chromatography

Keywords

Conditionally-active antibody

Activatable antibody

Masked antibody

Polymer-protein conjugate

Light-sensitivity

Protease-sensitivity

Data availability

Data will be made available on request.

© 2023 The Authors. Published by Elsevier B.V.

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