Remember me
European Surgical Research
Research Article
van den Akker E. · Dor F.J.M.F. · IJzermans J.N.M. · de Bruin R.
Introduction Short-term fasting protects against renal ischemia reperfusion injury (IRI). mTOR signaling is downregulated and may be involved in its protective effect. Rapamycin is considered a possible mimetic as it inhibits the mTOR-pathway. This study examines the effect of rapamycin on renal IRI. Methods Mice were divided in four groups : Ad libitum(AL), Fasted (F), AL treated with rapamycin (AL+R) and F treated with rapamycin (F+R). Rapamycin was administered intraperitoneally 24h before bilateral renal IRI was induced. Survival was monitored for 7 days. Renal cell death, regeneration, and mTOR activity were determined 48h after reperfusion. Oxidative stress resistance of HK-2 and PTEC cells after rapamycin treatment was determined. Results All F and F+R mice survived the experiment. Although rapamycin substantially downregulated mTOR-activity, survival in the AL+R group was similar to AL (10%). Renal regeneration was significantly reduced in AL+R but not in F+R. After IRI (48h), pS6K/S6K ratio was lower in F, F+R and AL+R groups compared to AL fed animals (p=0.02). In vitro, rapamycin also significantly downregulated mTOR-activity (p
The Author(s). Published by S. Karger AG, Basel
Article / Publication Details Open Access License / Drug Dosage / Disclaimer This article is licensed under the Creative Commons Attribution 4.0 International License (CC BY). Usage, derivative works and distribution are permitted provided that proper credit is given to the author and the original publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
Comments (0)