Experimental Nephrology and Genetics: Research Article

Chen C. · Wang H. · He X. · Li N. · Yuan F. · Xu J. · Jiang C.

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Article / Publication Details Abstract

Background: Obtaining sufficient renal tubular epithelial cells (RTCs) and maintaining the functions of RTCs are vital for developing a bioartificial renal tubule-assisted device for continuous renal replacement therapy. Methods: We established an optimal Transwell co-culture system using human primary renal proximal tubule epithelial cells (RPTECs) and bone marrow mesenchymal stem cells (BMMSCs) at different cell ratios to investigate morphological and functional changes in RTCs. Changes in cell proliferation, megalin expression, cell cycle, apoptosis, and levels of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-7 (BMP-7) after cell culture were investigated. Results: RPTEC/BMMSC co-culture at a cell ratio of 3:1 resulted in optimal morphology, function, and growth of RPTECs, in which, viability, proliferation, cytochrome P450 activity, and megalin expression in RPTECs were significantly increased compared to those in other co-cultures or RPTECs alone. Additionally, IGF-1 and BMP-7 levels were significantly higher in the 3:1 RPTEC/BMMSC co-culture than in the RPTECs alone. Conclusion: Our results demonstrate that co-culture with RPTECs has great potential for use in renal replacement therapy, thereby providing fundamental information for manufacturing a bioartificial kidney.

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