Figure 1. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human keratinocyte HaCaT cells. (A) HaCaT cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HaCaT cells were irradiated with UVB (30 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HaCaT cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (30 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HaCaT cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Figure 1. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human keratinocyte HaCaT cells. (A) HaCaT cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HaCaT cells were irradiated with UVB (30 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HaCaT cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (30 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HaCaT cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Figure 2. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. (A) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HDF cells were irradiated with UVB (100 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Figure 2. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. (A) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HDF cells were irradiated with UVB (100 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Figure 3. Moisture-recovery effects of YJP-EA, Hex, and BuOH on UVB-damaged HaCaT and HDF cells. (A,B) HaCaT and HDF cells were irradiated with UVB (30 or 100 mJ/cm2) and treated with YJP-EA, Hex, and BuOH for 24 h. The hyaluronic acid was measured using a hyaluronan ELISA kit and detected with 450 nm. (C,D) The protein-expression levels of filaggrin and SPT were analyzed by Western blot analysis. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells.

Figure 3. Moisture-recovery effects of YJP-EA, Hex, and BuOH on UVB-damaged HaCaT and HDF cells. (A,B) HaCaT and HDF cells were irradiated with UVB (30 or 100 mJ/cm2) and treated with YJP-EA, Hex, and BuOH for 24 h. The hyaluronic acid was measured using a hyaluronan ELISA kit and detected with 450 nm. (C,D) The protein-expression levels of filaggrin and SPT were analyzed by Western blot analysis. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells.

Figure 4. Melanin inhibition effects of YJP-EA, Hex, and BuOH on B16F10 cells. (A) B16F10 cells were treated with YJP-EA, Hex, and BuOH for 24 h. Next, the cell viability was measured using the MTT assay. (B) Melanin contents from α-MSH-stimulated B16F10 cell. The cells were treated with α-MSH (200 nM) and YJP-EA, Hex, and BuOH for 48 h. The cell lysates were measured at 490 nm. (C) To measure intracellular tyrosinase activity, the B16F10 cells were treated with -MSH (200 nM) and YJP-EA, Hex, and BuOH for 72 h. L-DOPA was added into the lysates and measured at 490 nm. (D) The mushroom tyrosinase activity was evaluated at -MSH (200 nM) and in YJP-EA-, Hex-, and BuOH-treated B16F10 cells. The supernatants with L-DOPA and tyrosinase were measured at 475 nm. (E) The whole cell lysates were analyzed by Western blot analysis. All experiments were performed individually in triplicate. *** p < 0.001 vs. α-MSH-treated cells, ** p < 0.01 vs. α-MSH-treated cells, and * p < 0.05 vs. α-MSH-treated cells. ###p < 0.001 vs. non-treated (NT) cells.

Figure 4. Melanin inhibition effects of YJP-EA, Hex, and BuOH on B16F10 cells. (A) B16F10 cells were treated with YJP-EA, Hex, and BuOH for 24 h. Next, the cell viability was measured using the MTT assay. (B) Melanin contents from α-MSH-stimulated B16F10 cell. The cells were treated with α-MSH (200 nM) and YJP-EA, Hex, and BuOH for 48 h. The cell lysates were measured at 490 nm. (C) To measure intracellular tyrosinase activity, the B16F10 cells were treated with -MSH (200 nM) and YJP-EA, Hex, and BuOH for 72 h. L-DOPA was added into the lysates and measured at 490 nm. (D) The mushroom tyrosinase activity was evaluated at -MSH (200 nM) and in YJP-EA-, Hex-, and BuOH-treated B16F10 cells. The supernatants with L-DOPA and tyrosinase were measured at 475 nm. (E) The whole cell lysates were analyzed by Western blot analysis. All experiments were performed individually in triplicate. *** p < 0.001 vs. α-MSH-treated cells, ** p < 0.01 vs. α-MSH-treated cells, and * p < 0.05 vs. α-MSH-treated cells. ###p < 0.001 vs. non-treated (NT) cells.

Figure 5. Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. (A–C) The ROS production was analyzed by flow cytometer. (D–F) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ###p < 0.001 vs. non-treated (NT) cells, ##p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.

Figure 5. Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. (A–C) The ROS production was analyzed by flow cytometer. (D–F) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ###p < 0.001 vs. non-treated (NT) cells, ##p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.