Q. Zhu1 and P. Jia2

Author information

1Department of Thoracic Surgery, Shandong Provincial Qianfoshan Hospital, the First Hospital Affiliated with Shandong First Medical University, Jinan, Shandong Province, China;
2Department of Respiratory and Critical Illness, Jinan Central Hospital Affiliated to Shandong First Medical University, Lixia District, Jinan, Shandong Province, China

Abstract

Different types of lung cancer, such as nonsmall-cell lung carcinoma (NSCLC), show the specific expression of long non-coding ribonucleic acids (LncRNAs). There is evidence showing the possible correlation between the expression of LncRNAs in tumor tissues and plasma exosomes. The present study aimed to evaluate the expression level of LncRNAs in NSCLC patients in the cancerous tissues and adjacent non-cancerous tissue and the circulating exosomes. The tumor and adjacent non-cancerous tissue samples of 142 patients with NSCLC were collected. Extracellular vesicles were isolated by the ultracentrifuge method, and the nanoparticle tracking and the flow cytometry analysis were performed to identify the exosome fraction. The relative expression of the five LncRNAs, including AFAP1-AS1, BANCR, ANRIL, CCAT1, and GAS5, between tumor tissue, adjacent non-cancerous tissue, and plasma exosomes were evaluated by quantitative real-time RT-PCR. Among 142 NSCLC samples, the expression of lncRNA-GAS5 in tumor tissue was correlated with lncRNA-ANRIL in tumor tissue (p=0.035, C=0.177), while the rest of the lncRNAs did not show a significant correlation in tumor tissue. Expression of the five different lncRNAs did not show any correlation in normal tissue and exosome content. Among the five lncRNAs, only lncRNA-BANCR expression in ANCT was correlated with the exosomal expression (p=0.027, C=0.186). In conclusion, the present research demonstrated that a panel consisting of these five lncRNAs could be helpful in the diagnosis of NSCLC.

Keywords:

Lung cancer, Exosome, LncRNAs, NSCLC

Publication type

Journal Article

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