Identification of antibacterial compounds in various Perillae folium chemotypes by high-performance thin-layer chromatography‒bioassay‒mass spectrometry

China Pharmacopoeia Commission (2025) Pharmacopoeia of the People’s Republic of China. Part 1. Chinese Medicines and Technology Press, Beijing

Kagawa N, Iguchi H, Henzan M, Hanaoka M (2019) Drying the leaves of Perilla frutescens increases their content of anticancer nutraceuticals. Food Sci Nutr 7(4):1494–1501. https://doi.org/10.1002/fsn3.993

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yang JH, Yoo JM, Lee E, Lee B, Cho WK, Park KI, Yeul MJ (2018) Anti-inflammatory effects of Perillae herba ethanolic extract against TNF-α/IFN-γ-stimulated human keratinocyte HaCaT cells. J Ethnopharmacol 211:217–223. https://doi.org/10.1016/j.jep.2017.09.041

Article  CAS  PubMed  Google Scholar 

Wei CL, Zhang CW, Guo BL, Li WP, Gao ZX, Zhang F, Tian J (2017) Research on effects of chemotype and components of Perilla frutescens leaf volatile oil I: different phenological periods. China J Chin Mater Med 42(04):712–718. https://doi.org/10.19540/j.cnki.cjcmm.20161222.078

Article  Google Scholar 

Ghimire BK, Yoo JH, Yu CY, Chung IM (2017) GC–MS analysis of volatile compounds of Perilla frutescens Britton var. Japonica accessions: morphological and seasonal variability. Asian Pac J Tropical Med 10(7):643–651. https://doi.org/10.1016/j.apjtm.2017.07.004

Article  CAS  Google Scholar 

Wang M, Shen R, Wang X, Zhang J, Ma J, Shi Y, Xu Q, Guo C, Di J, Liu A, Chen S, Liu Y (2025) Evaluation of edible and medicinal varieties based on multidimensional quantitative data: a case study of perilla leaf. J Food Compos Anal 140:107196. https://doi.org/10.1016/j.jfca.2025.107196

Article  CAS  Google Scholar 

Ahmed HM, Tavaszi-Sarosi S (2019) Identification and quantification of essential oil content and composition, total polyphenols and antioxidant capacity of Perilla frutescens (L.) Britt. Food Chem 275:730–738. https://doi.org/10.1016/j.foodchem.2018.09.155

Article  CAS  PubMed  Google Scholar 

Lim H, Shin S (2014) Anti-Bacillus and anti-Shigella activities of the essential oil from Perilla fruescens var. japonica Hara. J Essent Oil Bear Plants 17(2):309–316. https://doi.org/10.1080/0972060X.2014.895157

Article  CAS  Google Scholar 

Lin S, Shao P, Ma X, Sun PL (2009) GC/MS analysis of volatile oil from Perrilla frutenscens L. and evaluation of its inhibiting ability to bacteria. J Nucl Agric Sci 23(03):477–481

Article  CAS  Google Scholar 

Li LX, Deng RR, Zhang SQ, Wei LJ, Wang YQ, Shen ZB (2025) Antibacterial activity of oil from Perillae folium against avian pathogenic Escherichia coli and its inhibitory effect on biofilm formation. Chin Tradit Herb Drugs 56(08):2840–2848

CAS  Google Scholar 

Ahmed HM, Al-Zubaidy AMA (2020) Exploring natural essential oil components and antibacterial activity of solvent extracts from twelve Perilla frutescens L. Genotypes Arabian J Chem 13(10):7390–7402. https://doi.org/10.1016/j.arabjc.2020.08.016

Article  CAS  Google Scholar 

Chen J, Zhang D, Wang Q, Yang A, Zheng Y, Wang L (2022) Comprehensive comparison of two color varieties of Perillae folium by GC-MS-based metabolomic approach. Molecules 27(20):6792

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu J, Wan Y, Zhao Z, Chen H (2013) Determination of the content of rosmarinic acid by HPLC and analytical comparison of volatile constituents by GC-MS in different parts of Perilla frutescens (L.) Britt. Chem Cent J 7(1):61. https://doi.org/10.1186/1752-153X-7-61

Article  CAS  PubMed  PubMed Central  Google Scholar 

Si ZH, Kim MS, Pan L, Zhang PS, Gu LH, Wu LH, Yang L, Wang ZT (2025) Exploring characteristics and outlook of common stationary phases in thin-layer chromatography and their advantages in identification of traditional Chinese medicine. Chin J Pharm Anal 45(01):39–50. https://doi.org/10.16155/j.0254-1793.2024-0471

Article  Google Scholar 

Móricz ÁM, Szeremeta D, Knaś M, Długosz E, Ott PG, Kowalska T, Sajewicz M (2018) Antibacterial potential of the Cistus incanus L. phenolics as studied with use of thin-layer chromatography combined with direct bioautography and in situ hydrolysis. J Chromatogr A 1534:170–178. https://doi.org/10.1016/j.chroma.2017.12.056

Article  CAS  PubMed  Google Scholar 

Balázs VL, Horváth B, Kerekes E, Ács K, Kocsis B, Varga A, Böszörményi A, Nagy DU, Krisch J, Széchenyi A, Horváth G (2019) Anti-haemophilus activity of selected essential oils detected by TLC-direct bioautography and biofilm inhibition. Molecules 24(18):3301

Article  PubMed  PubMed Central  Google Scholar 

Goyal K, Tomar N, Singh AP, Sarin RK, Shukla SK (2020) Validation of an analytical method for the detection of ephedrine and its analogues in forensic samples using HPTLC–MS. JPC-J Planar Chromat 33(4):397–404. https://doi.org/10.1007/s00764-020-00049-6

Article  CAS  Google Scholar 

Hu HJ (2017) Comparison of chemical constitutions in different medicinal parts of perilla frutescens and establishment of international quality standard on Perillae folium. Shanghai Univ Tradit Chin Med. https://doi.org/10.27320/d.cnki.gszyu.2021.000485

Article  Google Scholar 

Sowers ME, Bethea E, Xiao W, Phase N, Harmon C, Jenkins D (2024) Quantification of isoniazid in tablets for tuberculosis treatment by thin layer chromatography with smartphone image capture and ImageJ analysis. Microchem J 202:110796. https://doi.org/10.1016/j.microc.2024.110796

Article  CAS  Google Scholar 

Japanese Pharmacopoeia Committee (2021) The Japanese pharmacopoeia, 18th edn. Society of Japanese Pharmacopoeia, Tokyo

Google Scholar 

Chen YS, Schwack W (2014) High-performance thin-layer chromatography screening of multi class antibiotics in animal food by bioluminescent bioautography and electrospray ionization mass spectrometry. J Chromatogr A 1356:249–257. https://doi.org/10.1016/j.chroma.2014.06.043

Article  CAS  PubMed  Google Scholar 

Jamshidi-Aidji M, Morlock GE (2015) Bioprofiling of unknown antibiotics in herbal extracts: development of a streamlined direct bioautography using Bacillus subtilis linked to mass spectrometry. J Chromatogr A 1420:110–118. https://doi.org/10.1016/j.chroma.2015.09.061

Article  CAS  PubMed  Google Scholar 

Yang F, Gu LH, Han ZZ, Wang ZT (2021) Rapid screening for natural lipase inhibitors from Alisma orientale combining high-performance thin-layer chromatography-bioautography with mass spectrometry. J Chromatogr B 1170:122599. https://doi.org/10.1016/j.jchromb.2021.122599

Article  CAS  Google Scholar 

Ge SH, Tian WQ, Lou ZY, Wang XX, Zhuang LL, Zhang J (2024) Long-term toxicity assessment of antibiotics against Vibrio fischeri: test method optimization and mixture toxicity prediction. J Hazard Mater 469:133933. https://doi.org/10.1016/j.jhazmat.2024.133933

Article  CAS  PubMed  Google Scholar 

Tang Y, Chen X, Sheng PN (2013) Research on the fingerprint of the volatile oil in Folium Perillae. Shanghai J Tradit Chin Med 47(09):82–86. https://doi.org/10.16305/j.1007-1334.2013.09.029

Article  Google Scholar 

Tada M, Okuno K, Chiba K, Ohnishi E, Yoshii T (1994) Antiviral diterpenes from Salvia officinalis. Phytochemistry 35(2):539–541. https://doi.org/10.1016/s0031-9422(00)94798-8

Article  CAS  Google Scholar 

Seo WH, Baek HH (2009) Characteristic aroma-active compounds of Korean Perilla (Perilla frutescens Britton) leaf. J Agric Food Chem 57(24):11537–11542. https://doi.org/10.1021/jf902669d

Article  CAS  PubMed  Google Scholar 

Chen WJ, Liu P, Fan S, Lin YQ, Zhao R (2017) Fragmentation pattern of 5 kinds of eugenol compounds by electrospray ionization mass spectrometry. J Food Saf Qual 8(09):3491–3498. https://doi.org/10.3969/j.issn.2095-0381.2017.09.036

Article  Google Scholar 

Fan R, Yang JH, Liu XH, Fu XS, Xu H (2012) Comparative analysis of volatile components of medicinal materials from Perilla frutescens. J Chin Med Mater 35(12):1961–1966. https://doi.org/10.13863/j.issn1001-4454.2012.12.027

Article  CAS  Google Scholar 

Yoo SS, Kook SH, Chin KB, Shim JH (2005) The comparison of physico-chemical and textural properties, as well as volatile compounds, from low-fat and regular-fat sausages. Int J Food Sci Technol 40(1):83–90. https://doi.org/10.1111/j.1365-2621.2004.00911.x

Article  CAS  Google Scholar 

Fang H, Han YY, Gu LH, Li LN, Yang L, Wu QG, Wang ZT (2022) Screening of acetylcholinesterase inhibitors in root of Acorus tatarinowii. Chin Tradit Pat Med 44(03):836–841. https://doi.org/10.3969/j.issn.1001-1528.2022.03.028

Article  Google Scholar 

Chowdhury JU, Bhuiyan MNI, Nandi NC (2008) Essential oil constituents of needles, dry needles, inflorescences and resins of Pinus caribaea Morelet growing in Bangladesh. Bangladesh J Botany 37(2):211–212. https://doi.org/10.3329/bjb.v37i2.1735

Comments (0)

No login
gif