Carvone is an important flavoring substance and widely used in the food and chemical industries. Carvone has two optical isomers (L-carvone and D-carvone), which have different aroma characteristics. However, the green extraction of natural carvone and isolation of chiral carvone have some difficulties, leading to its limited application. In this study, a deep eutectic solvent (DES) was prepared and used as a green extraction solvent for the extraction of carvone from herbaceous plants, then a chiral chromatography column combined with GC-MS were used for separation and detection of L-carvone and D-carvone. Response surface methodology was used to optimize the extraction conditions including volume of DES, extraction time and extraction temperature. The results showed that the extraction recoveries and intra-day precision (n=6) were 83.5%-101.3% and 3.2%-6.1% when the volume of DES was 5 mL, the extraction time and temperature were 25 min and 51 ℃, respectively. The MLOD and MLOQ of L-carvone and D-carvone were 8.0 mg/kg and 25.0 mg/kg, respectively. And the real sample detection results revealed that about 235.8-1600.0 mg/kg of L-carvone was detecetd in Mentha spicata L, and 6658.5-9788.6 mg/kg of D-carvone in Anethum graveolens L. seed. The established method can be an effective method for the detection of chiral carvone in herbaceous plants.

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