Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

Introduction: Genetic polymorphisms are considered potential causes of recurrent spontaneous abortions (RSA). This study aimed to determine the frequencies and association of rs1632944 and rs1632947 polymorphisms of the HLA-G gene promoter region with the RSA in women from northwest Iran.

Materials and Methods: This case-control study was conducted on 180 women who were referred to Madar Infertility Center and Al-Zahra Hospital in Tabriz. 90 patients with a history of at least two RSA as the patient group and 90 women with at least one child and no history of abortion as the control group was included in the study. 5 ml peripheral blood was obtained from each people, genomic DNA was extracted, amplified by PCR, and genotyped with sequencing and ARMS method. Data were analyzed with SPSS, Chi-square, and Fisher's exact tests.

Results: For rs1632944 polymorphism, frequencies of the AA, AG, and GG genotypes were 34.44%, 45.55%, and 20% for the control group, and 48.88%, 46.66%, and 4.44% for the patient group, respectively. We found that the minor allele G is recessive against major allele A and might protect women against RSA. For rs1632947 polymorphism, frequencies of the GG, AG, and AA genotypes were 52.22%, 35.55%, and 12.23% for the patient group and 64.44%, 32.22%, and 3.33% for the control group, respectively. We found that the minor allele A is recessive against the major allele G and is associated with the RSA.

Conclusion: The results showed that rs1632944 and rs1632947 polymorphism of the HLAG-G gene promoter might be related to RSA in women from Northwest Iran.