A novel system to determine activity of individual uridine 5'-diphospho-glucuronosyltransferase (UGT) isoforms: recombinant UGT-beads
Key Words
human glucuronosyltransferase
recombinant UGT
enzyme kinetics
magnetizable beads
rUGT-beads
BSA
fatty acids
immobilization
Abbreviationshuman liver microsomes
HLM
recombinant UGT SupersomeTM
rUGT microsomes
rUGT microsomes bound to magnetizable beads
rUGT-beads
50 mM Tris-HCl buffer pH 7.5 at 37°C
Tris-HCl buffer
uridine diphosphate glucuronic acid
UDPGA
UGT
uridine 5'-diphospho-glucuronosyltransferase
Vmax
maximum product formation rate normalized to mg of microsomal protein (pmol/min/mg protein)
© 2024 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.
留言 (0)