Behrens K, Alexander WS. Cytokine control of megakaryopoiesis. Growth Factors. 2018;36:89–103.

Article  CAS  PubMed  Google Scholar 

Gurney AL, Carver-Moore K, de Sauvage FJ, Moore MW. Thrombocytopenia in c-mpl-deficient mice. Science. 1994;265:1445–7.

Article  ADS  CAS  PubMed  Google Scholar 

de Sauvage FJ, Carver-Moore K, Luoh SM, Ryan A, Dowd M, Eaton DL, et al. Physiological regulation of early and late stages of megakaryocytopoiesis by thrombopoietin. J Exp Med. 1996;183:651–6.

Article  PubMed  Google Scholar 

Kimura S, Roberts AW, Metcalf D, Alexander WS. Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin. Proc Natl Acad Sci USA. 1998;95:1195–200.

Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

Levin J, Cocault L, Demerens C, Challier C, Pauchard M, Caen J, et al. Thrombocytopenic c-mpl(−/−) mice can produce a normal level of platelets after administration of 5-fluorouracil: the effect of age on the response. Blood. 2001;98:1019–27.

Article  CAS  PubMed  Google Scholar 

Tong W, Ibarra YM, Lodish HF. Signals emanating from the membrane proximal region of the thrombopoietin receptor (mpl) support hematopoietic stem cell self-renewal. Exp Hematol. 2007;35:1447–55.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qian H, Buza-Vidas N, Hyland CD, Jensen CT, Antonchuk J, Månsson R, et al. Critical role of thrombopoietin in maintaining adult quiescent hematopoietic stem cells. Cell Stem Cell. 2007;1:671–84.

Article  CAS  PubMed  Google Scholar 

Yoshihara H, Arai F, Hosokawa K, Hagiwara T, Takubo K, Nakamura Y, et al. Thrombopoietin/MPL signaling regulates hematopoietic stem cell quiescence and interaction with the osteoblastic niche. Cell Stem Cell. 2007;1:685–97.

Article  CAS  PubMed  Google Scholar 

Hitchcock IS, Kaushansky K. Thrombopoietin from beginning to end. Brit J Haematol. 2014;165:259–68.

Article  CAS  Google Scholar 

Morris R, Kershaw NJ, Babon JJ. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Sci. 2018;27:1984–2009.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Roy A, Shrivastva S, Naseer S. In and out: Traffic and dynamics of thrombopoietin receptor. J Cell Mol Med. 2021;25:9073–83.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Varghese LN, Defour J-P, Pecquet C, Constantinescu SN. The Thrombopoietin Receptor: Structural Basis of Traffic and Activation by Ligand, Mutations, Agonists, and Mutated Calreticulin. Front Endocrinol. 2017;8:59.

Article  Google Scholar 

Alexander WS, Maurer AB, Novak U, Harrison-Smith M. Tyrosine-599 of the c-Mpl receptor is required for Shc phosphorylation and the induction of cellular differentiation. EMBO J. 1996;15:6531–40.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Drachman JG, Kaushansky K. Dissecting the thrombopoietin receptor: functional elements of the Mpl cytoplasmic domain. Proc Natl Acad Sci USA. 1997;94:2350–5.

Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

Bouscary D, Lecoq-Lafon C, Chretien S, Zompi S, Fichelson S, Muller O, et al. Role of Gab proteins in phosphatidylinositol 3-kinase activation by thrombopoietin (Tpo). Oncogene. 2001;20:2197–204.

Article  CAS  PubMed  Google Scholar 

Rommel MGE, Hoerster K, Milde C, Schenk F, Roser L, Kohlscheen S, et al. Signaling properties of murine MPL and MPL mutants after stimulation with thrombopoietin and romiplostim. Exp Hematol. 2020;85:33–46.e36.

Article  CAS  PubMed  Google Scholar 

Lannutti BJ, Drachman JG. Lyn tyrosine kinase regulates thrombopoietin-induced proliferation of hematopoietic cell lines and primary megakaryocytic progenitors. Blood. 2004;103:3736–43.

Article  CAS  PubMed  Google Scholar 

Lv K, Jiang J, Donaghy R, Riling CR, Cheng Y, Chandra V, et al. CBL family E3 ubiquitin ligases control JAK2 ubiquitination and stability in hematopoietic stem cells and myeloid malignancies. Genes Dev. 2017;31:1007–23.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bersenev A, Wu C, Balcerek J, Tong W. Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2. J Clin Investig. 2008;118:2832–44.

CAS  PubMed  PubMed Central  Google Scholar 

Saur SJ, Sangkhae V, Geddis AE, Kaushansky K, Hitchcock IS. Ubiquitination and degradation of the thrombopoietin receptor c-Mpl. Blood. 2010;115:1254–63.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Donaghy R, Han X, Rozenova K, Lv K, Jiang Q, Doepner M, et al. The BRISC deubiquitinating enzyme complex limits hematopoietic stem cell expansion by regulating JAK2 K63-ubiquitination. Blood. 2019;133:1560–71.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hitchcock IS, Chen MM, King JR, Kaushansky K. YRRL motifs in the cytoplasmic domain of the thrombopoietin receptor regulate receptor internalization and degradation. Blood. 2008;112:2222–31.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sangkhae V, Saur SJ, Kaushansky A, Kaushansky K, Hitchcock IS. Phosphorylated c-Mpl tyrosine 591 regulates thrombopoietin-induced signaling. Exper Hematol. 2014;42:477–86.e474.

Article  CAS  Google Scholar 

Jung AS, Kaushansky A, Macbeath G, Kaushansky K. Tensin2 is a novel mediator in thrombopoietin (TPO)-induced cellular proliferation by promoting Akt signaling. Cell Cycle. 2011;10:1838–44.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pecquet C, Staerk J, Chaligne R, Goss V, Lee KA, Zhang X, et al. Induction of myeloproliferative disorder and myelofibrosis by thrombopoietin receptor W515 mutants is mediated by cytosolic tyrosine 112 of the receptor. Blood. 2010;115:1037–48.

Article  CAS  PubMed  Google Scholar 

Klampfl T, Gisslinger H, Harutyunyan AS, Nivarthi H, Rumi E, Milosevic JD, et al. Somatic mutations of calreticulin in myeloproliferative neoplasms. N Engl J Med. 2013;369:2379–90.

Article  CAS  PubMed  Google Scholar 

Lu X, Levine R, Tong W, Wernig G, Pikman Y, Zarnegar S, et al. Expression of a homodimeric type I cytokine receptor is required for JAK2V617F-mediated transformation. Proc Natl Acad Sci USA. 2005;102:18962–7.

Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

Sangkhae V, Etheridge SL, Kaushansky K, Hitchcock IS. The thrombopoietin receptor, MPL, is critical for development of a JAK2V617F-induced myeloproliferative neoplasm. Blood. 2014;124:3956–63.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Elf S, Abdelfattah NS, Chen E, Perales-Patón J, Rosen EA, Ko A, et al. Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation. Cancer Discov. 2016;6:368–81.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Marty C, Pecquet C, Nivarthi H, El-Khoury M, Chachoua I, Tulliez M, et al. Calreticulin mutants in mice induce an MPL-dependent thrombocytosis with frequent progression to myelofibrosis. Blood. 2016;127:1317–24.

Article  CAS  PubMed  Google Scholar 

Lu X, Huang LJ-S, Lodish HF. Dimerization by a cytokine receptor is necessary for constitutive activation of JAK2V617F. J Biological Chem. 2008;283:5258–66.

Article  CAS  Google Scholar 

Chachoua I, Pecquet C, El-Khoury M, Nivarthi H, Albu R-I, Marty C, et al. Thrombopoietin receptor activation by myeloproliferative neoplasm associated calreticulin mutants. Blood. 2016;127:1325–35.

Article  CAS  PubMed  Google Scholar 

Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, et al. Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Blood. 2016;127:1307–16.

Article  CAS  PubMed  Google Scholar 

Fielder PJ, Gurney AL, Stefanich E, Marian M, Moore MW, Carver-Moore K, et al. Regulation of thrombopoietin levels by c-mpl-mediated binding to platelets. Blood. 1996;87:2154–61.

Article  CAS  PubMed  Google Scholar 

Oguro H, Ding L, Morrison SJ. SLAM Family Markers Resolve Functionally Distinct Subpopulations of Hematopoietic Stem Cells and Multipotent Progenitors. STEM. 2013;13:102–16.