Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells

BAEE

N-benzoyl-L-arginine ethyl ester

bSDB

sulfonated polystyrenedivinylbenzene

BLNA

N-benzoyl-lysine p-nitroanilide

CHAPS

(3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

CVs

Coefficients of Variation

DIA

data independent acquisition

DDA

data dependent acquisition

DDM

n-dodecyl-β-D-maltoside

FASP

filter-aided sample preparation

FracFD

fractional factorial design

GRAVY

grand average of hydropathicity

GdnHCl

guanidine hydrochloride

HEPES

(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)

HILIC

Hydrophilic Interaction Liquid Chromatography

iProChip

integrated proteomics chip

iTRAQ

Isobaric Tag for Relative and Absolute Quantitation

ITC

isothermal titration calorimetry

LFQ

Label-free Quantification

mPOP

Minimal ProteOmic Sample Preparation

MMTS

methyl methanethiosulfonate

MRM

Multiple Reaction Monitoring

MS/MS

tandem mass spectrometry

nanoPOTS

nanodroplet processing in one pot for trace samples

OCT

optimum cutting temperature

PBS

phosphate-buffered saline

PC-IDMS

protein cleavage isotope dilution mass spectrometry

PCT

pressure cycling technology

PTMs

post-translational modifications

RIPA

Radioimmunoprecipitation assay

RP-HPLC

reversed phase high performance liquid chromatography

SCIProChip

single-cell proteomics chip

SCoPE-MS

Single Cell ProtEomics by Mass Spectrometry

SCP

single cell proteomics

SCX

strong cation exchange

SLS

sodium lauroyl sarcosinate

SPE

solid phase extraction

SP3

Single-Pot Solid-Phase-enhanced Sample Preparation

SPEED

Sample Preparation by Easy Extraction and Digestion

SWATH-MS

Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectra

TCEP

Tris(2-carboxyethyl)phosphine

TEAB

Triethylammonium bicarbonate

TPCK

1-chloro-3-tosylamido-4-phenyl-2-butanone

Tris(HCl)

Tris(hydroxymethyl)aminomethane hydrochloride

T-SCP

true single cell-derived proteomics

UADs

ultrasonically-assisted digests

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