CSF1R-mediated myeloid cell depletion shifts the ratio of motor cortical excitatory to inhibitory neurons in a multiple system atrophy model

Keywords

Colony-stimulating factor 1 receptor

Excitation-inhibition balance

GABAergic interneurons

Microglia-neuron-interaction

Motor cortex

Multiple system atrophy

PLX5622

Pyramidal neurons

AbbreviationsAMPAR

α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor

CSF1R

colony-stimulating factor 1 receptor

CSF1R +/− mice

colony-stimulating factor 1 receptor haploinsufficient mice

CTIP2+

COUP TF1 interacting protein 2-positive

DAB

3,3′-diaminobenzidin

DAPI

4′,6-diamidino-2-phenylindole

GABAAR

gamma-aminobutyric acid A receptor

GABABR1

gamma-aminobutyric acid B receptor 1

GABAergic

gamma-aminobutyric acid-positive

GAD67

glutamic acid decarboxylase 67

GluR2

glutamate ionotropic receptor α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid type subunit 2

hα-syn

human α-synuclein

Iba1

ionized calcium-binding adapter molecule 1

MSA

multiple system atrophy

NMDAR

N-Methyl-d-aspartate receptor

Olig2

oligodendrocyte transcription factor 2

PLX5622

small molecule inhibitor of the colony-stimulating factor 1 receptor

PSD-95

postsynaptic density protein 95

RIPA

radioimmunoprecipitation assay

RRID

Research Resource Identification

shank2

SH3 and multiple ankyrin repeat domains protein 2

SNAP-25

synaptosome-associated protein 25

WFA

Wisteria Floribunda Agglutinin

Data availability

Data supporting the findings are available from the corresponding authors upon reasonable request.

© 2024 Published by Elsevier Inc.

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