Patch-seq: Advances and Biological Applications

Allen DE, Donohue KC, Cadwell CR, Shin D, Keefe MG, Sohal VS, Nowakowski TJ (2022) Fate mapping of neural stem cell niches reveals distinct origins of human cortical astrocytes. Science 376(6600):1441–1446. https://doi.org/10.1126/science.abm5224

Article  CAS  PubMed  PubMed Central  Google Scholar 

Arman AC, Sampath AP (2010) Patch clamp recordings from mouse retinal neurons in a dark-adapted slice preparation. J vis Exp: Jove. https://doi.org/10.3791/2107

Article  PubMed  PubMed Central  Google Scholar 

Arroyo S, Bennett C, Hestrin S (2018) Correlation of synaptic inputs in the visual cortex of awake, behaving mice. Neuron 99(6):1289-1301.e2. https://doi.org/10.1016/j.neuron.2018.08.008

Article  CAS  PubMed  PubMed Central  Google Scholar 

Arulkandarajah KH, Osterstock G, Lafont A, Le Corronc H, Escalas N, Corsini S, Le Bras B et al (2021) Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord. Curr Biol: CB 31(20):4584–95.e4. https://doi.org/10.1016/j.cub.2021.08.019

Article  CAS  PubMed  Google Scholar 

Bakken TE, Jorstad NL, Qiwen Hu, Lake BB, Tian W, Kalmbach BE, Crow M et al (2021) Comparative cellular analysis of motor cortex in human, marmoset and mouse. Nature 598(7879):111–119. https://doi.org/10.1038/s41586-021-03465-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bardy C, van den Hurk M, Kakaradov B, Erwin JA, Jaeger BN, Hernandez RV, Eames T et al (2016) Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology. Mol Psychiatry 21(11):1573–1588. https://doi.org/10.1038/mp.2016.158

Article  CAS  PubMed  PubMed Central  Google Scholar 

Berg J, Sorensen SA, Ting JT, Miller JA, Chartrand T, Buchin A, Bakken TE et al (2021) Human neocortical expansion involves glutamatergic neuron diversification. Nature 598(7879):151–158. https://doi.org/10.1038/s41586-021-03813-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brecht M, Sakmann B (2002) Dynamic representation of whisker deflection by synaptic potentials in spiny stellate and pyramidal cells in the barrels and septa of layer 4 rat somatosensory cortex. J Physiol 543(Pt 1):49–70. https://doi.org/10.1113/jphysiol.2002.018465

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cadwell CR, Palasantza A, Jiang X, Berens P, Deng Q, Yilmaz M, Reimer J et al (2016) Electrophysiological, transcriptomic and morphologic profiling of single neurons using patch-seq. Nat Biotechnol 34(2):199–203. https://doi.org/10.1038/nbt.3445

Article  CAS  PubMed  Google Scholar 

Cadwell CR, Sandberg R, Jiang X, Tolias AS (2017a) Q&A: using patch-seq to profile single cells. BMC Biol 15(1):58. https://doi.org/10.1186/s12915-017-0396-0

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cadwell CR, Scala F, Li S, Livrizzi G, Shen S, Sandberg R, Jiang X, Tolias AS (2017b) Multimodal profiling of single-cell morphology, electrophysiology, and gene expression using patch-seq. Nat Protoc 12(12):2531–2553. https://doi.org/10.1038/nprot.2017.120

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cadwell CR, Scala F, Fahey PG, Kobak D, Mulherkar S, Sinz FH, Papadopoulos S et al (2020) Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex. Elife. https://doi.org/10.7554/eLife.52951

Article  PubMed  PubMed Central  Google Scholar 

Camunas-Soler J, Dai X-Q, Hang Y, Bautista A, Lyon J, Suzuki K, Kim SK, Quake SR, MacDonald PE (2020) Patch-seq links single-cell transcriptomes to human islet dysfunction in diabetes. Cell Metab 31(5):1017–31.e4. https://doi.org/10.1016/j.cmet.2020.04.005

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cauli B, Porter JT, Tsuzuki K, Lambolez B, Rossier J, Quenet B, Audinat E (2000) Classification of fusiform neocortical interneurons based on unsupervised clustering. Proc Natl Acad Sci USA 97(11):6144–6149. https://doi.org/10.1073/pnas.97.11.6144

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cauli B, Tong X-K, Rancillac A, Serluca N, Lambolez B, Rossier J, Hamel E (2004) Cortical GABA interneurons in neurovascular coupling: relays for subcortical vasoactive pathways. J Neurosci: off J Soc Neurosci 24(41):8940–8949. https://doi.org/10.1523/JNEUROSCI.3065-04.2004

Article  CAS  Google Scholar 

Chartrand T, Dalley R, Close J, Goriounova NA, Lee BR, Mann R, Miller JA et al (2023) Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex. Science 382(6667):eadf0805. https://doi.org/10.1126/science.adf0805

Article  CAS  PubMed  Google Scholar 

Chen X, Zhang K, Zhou L, Gao X, Wang J, Yao Y, He F et al (2016) Coupled electrophysiological recording and single cell transcriptome analyses revealed molecular mechanisms underlying neuronal maturation. Protein Cell 7(3):175–186. https://doi.org/10.1007/s13238-016-0247-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dai X-Q, Camunas-Soler J, Briant LJB, Dos Santos T, Spigelman AF, Walker EM, Drigo RAE et al (2022) Heterogenous impairment of α cell function in type 2 diabetes is linked to cell maturation state. Cell Metab 34(2):256–68.e5. https://doi.org/10.1016/j.cmet.2021.12.021

Article  CAS  PubMed  PubMed Central  Google Scholar 

Deng P, Xu ZC (2012) Whole-cell patch-clamp recordings on spinal cord slices. Methods Mol Biol 851:65–72. https://doi.org/10.1007/978-1-61779-561-9_4

Article  CAS  PubMed  Google Scholar 

Duguid I, Branco T, London M, Chadderton P, Häusser M (2012) Tonic inhibition enhances fidelity of sensory information transmission in the cerebellar cortex. J Neurosci: off J Soc Neurosci 32(32):11132–11143. https://doi.org/10.1523/JNEUROSCI.0460-12.2012

Article  CAS  Google Scholar 

Duguid I, Branco T, Chadderton P, Arlt C, Powell K, Häusser M (2015) Control of cerebellar granule cell output by sensory-evoked golgi cell inhibition. Proc Natl Acad Sci USA 112(42):13099–13104. https://doi.org/10.1073/pnas.1510249112

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ellender TJ, Avery SV, Mahfooz K, Scaber J, von Klemperer A, Nixon SL, Buchan MJ et al (2019) Embryonic Progenitor Pools Generate Diversity in Fine-Scale Excitatory Cortical Subnetworks. Nat Commun 10(1):5224. https://doi.org/10.1038/s41467-019-13206-1

Article  CAS  PubMed  PubMed Central  Google Scholar 

Földy C, Darmanis S, Aoto J, Malenka RC, Quake SR, Südhof TC (2016) Single-cell RNAseq reveals cell adhesion molecule profiles in electrophysiologically defined neurons. Proc Natl Acad Sci USA 113(35):E5222–E5231. https://doi.org/10.1073/pnas.1610155113

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fuzik J, Zeisel A, Máté Z, Calvigioni D, Yanagawa Y, Szabó G, Linnarsson S, Harkany T (2016) Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes. Nat Biotechnol 34(2):175–183. https://doi.org/10.1038/nbt.3443

Article  CAS  PubMed  Google Scholar 

Fuzik J, Rehman S, Girach F, Miklosi AG, Korchynska S, Arque G, Romanov RA et al (2019) Brain-wide genetic mapping identifies the indusium griseum as a prenatal target of pharmacologically unrelated psychostimulants. Proc Natl Acad Sci USA 116(51):25958–25967. https://doi.org/10.1073/pnas.1904006116

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gouwens NW, Sorensen SA, Baftizadeh F, Budzillo A, Lee BR, Jarsky T, Alfiler L et al (2020) Integrated morphoelectric and transcriptomic classification of cortical GABAergic cells. Cell 183(4):935–53.e19. https://doi.org/10.1016/j.cell.2020.09.057

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hao Z-Z, Wei J-R, Xiao D, Liu R, Nana Xu, Tang L, Huang M et al (2022) Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations. Nat Neurosci 25(6):805–817. https://doi.org/10.1038/s41593-022-01073-x

Article  CAS  PubMed  Google Scholar 

He Y, Pingwen Xu, Wang C, Xia Y, Meng Yu, Yang Y, Kaifan Yu et al (2020) Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance. Nat Commun 11(1):2165. https://doi.org/10.1038/s41467-020-15982-7

Article  CAS  PubMed  PubMed Central  Google Scholar 

Huang W, Qiang Xu, Jing Su, Tang L, Hao Z-Z, Chuan Xu, Liu R et al (2022) Linking transcriptomes with morphological and functional phenotypes in mammalian retinal ganglion cells. Cell Rep 40(11):111322. https://doi.org/10.1016/j.celrep.2022.111322

Article  CAS  PubMed  Google Scholar 

Ishikawa T, Shimuta M, Häusser M (2015) Multimodal sensory integration in single cerebellar granule cells in vivo. Elife. https://doi.org/10.7554/eLife.12916

Article  PubMed  PubMed Central  Google Scholar 

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