Redox potentials elucidate the electron transfer pathway of NAD+-dependent formate dehydrogenases

bis-MGD

bis-metal-binding pterin guanine dinucleotide

CAPS

N-cyclohexyl-3-aminopropanesulfonic acid

cryo-EM

cryogenic electron microscopy

DTPA

diethylenetriamine pentaacetate

DCPIP

2,6-dichlorophenolindophenol

EDTA

ethylenediaminetetraacetic acid

EPR

electron paramagnetic resonance

FMN

riboflavin-5′-phosphate

HDCR

hydrogen-dependent CO2 reductase

NAD+

nicotinamide adenine dinucleotide (oxidized form)

NADH

nicotinamide adenine dinucleotide (reduced form)

SDS-PAGE

sodium dodecylsulfate polyacrylamide gel electrophoresis

SHE

standard hydrogen electrode

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