Available online 7 December 2023, 103926

Author links open overlay panelABSTRACTAIM

Assessment of the impact of contemporary disinfection techniques Moringa Oleifera (M. Oleifera), NanoCare Plus Silver Gold® (Nanocare), and Fullerene (C60) on survival rates of S.Mutans and shear bond strength (SBS) of composite to the carious affected dentin (CAD)

MATERIAL AND METHODS

Sixty mandibular molars having caries progression till the middle 1/3rd of the dentin were disinfected. The CAD surface of twenty samples was inoculated with S.mutans. Based on different disinfection regimens samples were arbitrarily assigned to four groups (n:15) Group 1: 2% CHX, Group 2:NanoCare, Group 3:Fullerene (C60), and Group 4 (M. Oleifera. This was followed by calculating the survival rate of S.mutans. Ten samples from each group were then restored with composite restoration and thermocycled. Assessment of SBS and failure mode was performed using a universal testing machine and stereomicroscope at 40X magnification. Statistical significance among groups was assessed using analysis of variance (ANOVA) and Tukey's test at a significance level of p=0.05

RESULTS

Group 2 samples treated with NanoCare exhibited the lowest survival rate (0.39 ±0.02 CFU/ml) of S.Mutans. However, Group 1 (CHX) samples exhibited the highest survival count (0.51±0.10 CFU/ml). Furthermore, the highest composite to CAD bond was observed in Group 3 Fullerene(C60) (18.44±0.25 MPa) samples and the lowest SBS was observed in Group 1 (CHX) (12.48±1.69 MPa)

CONCLUSION

Fullerene(C60) and Moringa Oleifera extract hold promise as potential substitutes for chlorhexidine (CHX) in clinical applications, offering the potential for improved S.Mutans elimination and enhanced bond strength to CAD surface

Section snippetsINTRODUCTION

The partial caries removal technique (PCR) is a recently developed approach to restore dental cavities[1]. This treatment entails the thorough removal of all infected tissues except for the carious-affected dentin (CAD) that is present near the pulp[1]. S.Mutans are highly responsible for the development of dental caries[1]. However, the decision to utilize CAD surface for adhesion of restorative material has resulted in some loss of bonding capacity thereby impacting the overall durability and

Extraction from the leaf of M.Oleifera

The present in-vitro study was conducted at the Centre for Specialist Dental Practice and Clinical Research. Riyadh. Saudi Arabia and completed in three months. The study was approved by the ethical board IRB# F9-948×8 on 3rd June 2023. For preparation of M. Oleifera leaf extract, a mixing of 250 mL of ethyl alcohol (EtOH) and 50 grams of powdered leaves was performed. The resulting combination was then incubated for a time duration of 6 hours while maintaining constant agitation. The extract

RESULTS

Survival rates of S.Mutans after the application of various cavity disinfection regimes to the CAD surface. The results indicated that Group 2 samples that were exposed to NanoCare displayed the lowest survival rate (0.39 ±0.02 CFU/ml) of S.Mutans. However, the Group 1 samples pre-treated with CHX exhibited the highest survival count (0.51±0.10 CFU/ml). Intergroup comparison analysis revealed that Group 2, Group 3 (C60) (0.45± 0.06 CFU/ml), and Group 4 (M. Oleifera) (0.43 ± 0.04 CFU/ml)

DISCUSSION

The laboratory-based inquiry was conducted to test the hypothesis that there will be no significant difference in the survival rates of S.mutans on the CAD surface after employing various contemporary disinfection strategies, such as NanoCare, Fullerene (C60), and M.Oleifera extract as compared to the traditional control method CHX. Additionally, it was also anticipated that specimens treated with NanoCare, Fullerene (C60), and M.Oleifera leaf extract would exhibit comparable bond strength

CONCLUSION

Fullerene (C60) and Moringa oleifera extract hold promise as potential substitutes for chlorhexidine (CHX) in clinical applications, offering the potential for improved S.Mutans elimination and enhanced bond strength. Nonetheless, it is essential to underscore that additional extensive research and studies are needed to substantiate these benefits conclusively

CRediT authorship contribution statement

Yasser F. Al Fawaz: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing.

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