Proper nucleoid distribution is necessary for the homogenous distribution of mitochondrial proteins, with defects that affect nucleoid distribution resulting in the irregular distribution of proteins within mitochondria (Ishihara et al., 2015, Silva Ramos et al., 2019). The pattern of nucleoid distribution was characterized in yeast and mammalian cells either manually or using scripts (Iborra et al., 2004, Jajoo et al., 2016, Kukat et al., 2011, Osman et al., 2015, Tauber et al., 2013). We also