Available online 11 November 2023, 101952

Author links open overlay panel, , , ABSTRACTBACKGROUND

In order to achieve uneventful and rapid healing of the tissues, a suture material should be biocompatible, easy to handle, sterile, and have good and uniform tensile strength. Hence, in the present study, characterisation of the suture materials was done through a novel green chemistry approach using Punica granatum seed extract.

MATERIALS AND METHODS

Ethanolic extract of Punica granatum seed was prepared by dissolving 25g of Punica granatum seed powder with 100ml of ethanol. The obtained extract was coated in Silk and Vicryl suture material and was tested for its surface morphology (SEM), tensile strength, anti microbial activity, biocompatibility and wound healing potential.

RESULTS

Silk and vicryl sutures coated with Punica granatum seed extract showed the uniform coating and deposition of extract with sustaining integrity. Vicryl suture coated with the extract had good tensile strength and antimicrobial activity. The in vitro scratch assay and biocompatibility test showed that the Punica granatum seed extract had excellent wound healing potential and can be used without any effect on the viability of the normal cells.

CONCLUSION

Within the limitations of the study it can be concluded that Punica granatum seed extract coated Vicryl sutures had good tensile strength and anti microbial activity. Punica granatum seed extract also showed excellent biocompatibility and wound healing potential.

Section snippetsINTRODUCTION

Suture materials play an essential and vital role in tissue healing process, its reconstruction and reassembly separated due to surgical procedures or trauma.1,2. In order to achieve uneventful and rapid healing of the tissues, a suture material should be biocompatible, easy to handle, sterile, and have good and uniform tensile strength3,4.

With respect to its origin and biological properties, suture materials can be classified into natural or synthetic sutures and resorbable or non-resorbable

MATERIALS AND METHODOLOGY

All the chemicals used in the study are analytical grade.

Morphological characterisation

SEM micrographs analysis of the silk suture material showed a typical multi filamentous structure (Figure 5), whereas Vicryl suture showed a mono filamentous structure (Figure 6). Punica granatum seed extract deposition was observed on the coated silk (Figure 7) and vicryl suture material (Figure 8), respectively.

Tensile strength

Control and coated silk and Vicryl suture materials were intact and suitable for mechanical testing. Each sample showed an obvious breaking point during the testing. The Maximum force

DISCUSSION

In the present study, characterisation of silk and vicryl suture coated with Punica granatum seed extract was done and to date, no other studies have been reported so far with Punica granatum seed extract.

Under morphological characterisation in the present study, coated silk and Vicryl sutures showed uniformly coated surfaces with sustaining integrity by Punica granatum seed extract. Similar results were also reported by Sudha et al. 2017, where they have coated the silk suture material with

CONCLUSION

Characterisation of silk and vicryl sutures coated with Punica granatum seed extract showed the uniform coating and deposition of extract with sustaining integrity. Vicryl suture coated with the extract had good tensile strength when compared to the coated silk suture. Vicryl suture coated with the extract also showed antimicrobial activity against Candida albicans and Staphylococcus aureus. The in vitro scratch assay and biocompatibility test showed that the Punica granatum seed extract had

CRediT authorship contribution statement

P.E. Jai Rexlin: Formal analysis, Investigation, Data curation, Writing – original draft, Visualization. Anju Cecil: Conceptualization, Writing – review & editing, Supervision. Rajalakshmanan Eswaramoorthy: Methodology, Validation, Resources, Investigation, Data curation, Writing – review & editing. Rubin S John: Writing – review & editing, Supervision.

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