Int J Sports Med
DOI: 10.1055/a-2172-8171
Physiology & Biochemistry
Qihai Xie
1
Department of Cardiology, the First Affiliated Hospital of Soochow
University, Suzhou, China
2
Department of Cardiology, Baoshan Branch, Shuguang Hospital Affiliated
to Shanghai University of Traditional Chinese Medicine, Shanghai,
China
,
Xiangdong Xu
3
Department of Cardiology, Jiading District Central Hospital Affiliated
Shanghai University of Medicine & Health Sciences, Shanghai,
China
,
Danqun Xiong
3
Department of Cardiology, Jiading District Central Hospital Affiliated
Shanghai University of Medicine & Health Sciences, Shanghai,
China
,
Man Yao
3
Department of Cardiology, Jiading District Central Hospital Affiliated
Shanghai University of Medicine & Health Sciences, Shanghai,
China
,
Yafeng Zhou
1
Department of Cardiology, the First Affiliated Hospital of Soochow
University, Suzhou, China
› Author Affiliations
Funding Information
Jiangsu Province’s 333 High-Level Talents Project — BRA2017539
National Natural Science Foundation of China —
http://dx.doi.org/10.13039/501100001809; 81873486,
81770327
Jiangsu Province’s Key Provincial Talents Program — ZDRCA2016043
Natural Scientific Fund of Jiangsu Province — BK20161226
› Further Information
Also available at
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Abstract
Cardiac hypertrophy (CH) is an early marker in the clinical course of heart
failure. Circular RNAs (circRNAs) play important roles in human disease.
However, the role of circ_Larp4b in myocardial hypertrophy has not been studied.
Angiotensin II (Ang II) treated HL-1 cells to induce a CH cell model.
Quantitative real-time polymerase chain reaction was used to detect the
expression of circ_Larp4b, microRNA-298-5p, and myocyte enhancer factor 2
(Mef2c). Western blot detected the protein level of alpha-actinin-2 (ACTN2),
beta-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), and
Mef2c. The relationship between miR-298-5p and circ_Larp4b or Mef2c was verified
by dual-luciferase reporter assay and RNA pull-down assay. Circ_Larp4b and Mef2c
were upregulated in HL-1 cells treated with Ang II. Moreover, circ_Larp4b
down-regulation regulated the progress of CH induced by Ang II. MiR-298-5p was a
target of circ_Larp4b, and Mef2c was a target of miR-298-5p. Overexpressed Mef2c
reversed the cell size inhibited by miR-298-5p in Ang II-induced HL-1 cells.
Circ_Larp4b regulated CH progress by regulating miR-298-5p/Mef2c
axis.
Key words
circ_larp4b -
mir-298-5p -
cardiac hypertrophy -
mef2c -
skeletal muscle -
regeneration -
exercise physiology -
oxidative stress -
myosin
Publication History
Received: 08 November 2022
Accepted: 22 August 2023
Article published online:
13 November 2023
© 2023. Thieme. All rights reserved.
Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart,
Germany
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