Magnetic particles are commonly used to isolate specific cell types from blood samples. Residual particles present in the genomic DNA extracted from these cells can interfere with concentration measurements by UV absorbance spectrophotometry. In this study, we determined the degree of inaccuracy of DNA quantification by UV spectrophotometry, in the context of the lineage-specific chimerism analysis workflow. We showed that the presence of residual magnetic particles and RNA leads to an overestimation of the DNA concentration.

The authors have declared no competing interest.

Izza Usman Bajwa was supported by grants from BioAlberta and BioTalent Canada.

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The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

The Institutional Review Board of WCG IRB gave ethical approval for the collection and use for research of the blood samples used for this work.

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All data produced in the present study are available upon reasonable request to the authors.