Experimental Section: Research Article

Huang Q.a,b· Liu D.c· Cui S.b· Li P.b· Yin Z.b· Li D.d· Cao D.b· Zhang Y.b· Cai G.b· Chen X.b· Sun X.b

Author affiliations

aDepartment of Nephrology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
bDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, China
cDepartment of Nephrology, Air Force General Hospital, Chinese PLA, Beijing, China
dDepartment of Academic Research, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing, China

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Article / Publication Details

First-Page Preview

Received: May 17, 2022
Accepted: January 05, 2023
Published online: January 31, 2023

Number of Print Pages: 13
Number of Figures: 7
Number of Tables: 0

ISSN: 0304-324X (Print)
eISSN: 1423-0003 (Online)

For additional information: https://www.karger.com/GER

Abstract

Introduction: Youthful blood environment was shown to decelerate the aging process of the kidney and to attenuate senile renal fibrosis in a young-old parabiotic animal model; in addition, we identified a stem cell factor (SCF) that is closely linked with the process. This research was to investigate the effect of youthful blood environment on senile renal interstitial fibrosis and the role of SCF. Methods: We bred SCF receptor c-Kit gene loss-of-function Wps/Wps mice and established a combination mice model that was subjected to unilateral ureteral obstructive (UUO) and parabiotic surgeries. Parabiotic mice were divided into isochronic parabiotic (young-young [Y-IP] and old-old [O-IP]) and heterochronic parabiotic (young-old [HP]) groups. UUO surgery was performed in one of the parabiotic pairs in the IP group (Y-IPuuo and O-IPuuo) and in the elderly mice in the HP group (O-HPuuo). In order to study the role of SCF/c-kit on renal interstitial fibrosis, UUO surgery was performed in wildtype (WT) and Wps/Wps mice. Results: Fourteen days after UUO surgery, the kidney interstitial fibrosis area, kidney function, and the expressions of SCF/c-Kit, pNF-κB, and fibrosis-related proteins in the O-HPuuo group were significantly lower than those in the Ouuo and O-IPuuo groups. Compared with WT UUO mice, the expressions of pNF-κB and fibrosis-related proteins and the kidney function were all significantly decreased in Wps/Wps UUO mice. Conclusion: Youthful blood environment downregulated the expressions of SCF/c-Kit in elderly UUO mice and ameliorated UUO-induced kidney fibrosis and function loss.

© 2023 S. Karger AG, Basel

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First-Page Preview

Received: May 17, 2022
Accepted: January 05, 2023
Published online: January 31, 2023

Number of Print Pages: 13
Number of Figures: 7
Number of Tables: 0

ISSN: 0304-324X (Print)
eISSN: 1423-0003 (Online)

For additional information: https://www.karger.com/GER

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