Multiphoton scanning laser microscope based on femtosecond fiber laser

Alicja Kwaśny, Jakub Bogusławski, Grzegorz Soboń

Abstract
We present a multiphoton scanning laser microscope based on a femtosecond frequency-doubled erbium-doped fiber laser. The laser used in the epi-illumination microscope setup generated 95 fs pulses at the wavelength of 780 nm with 44.3 mW average power at 100 MHz pulse repetition rate. The imaging process was controlled by custom software developed in the NI LabVIEW environment. Detection of two-photon fluorescence was proven by acquiring a series of images from various biological samples.

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References

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Keywords

fiber lasers; multiphoton microscopy; biophotonics;

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Photonics Letters of Poland - A Publication of the Photonics Society of Poland
Published in cooperation with SPIE

ISSN: 2080-2242