Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

1. Watts, AM, Tyler, MP, Perry, ME, Roberts, AH, McGrouther, DA. Burn depth and its histological measurement. Burns. 2001;27:154-160.
Google Scholar | Crossref | Medline2. Evans, ND, Oreffo, RO, Healy, E, Thurner, PJ, Man, YH. Epithelial mechanobiology, skin wound healing, and the stem cell niche. J Mech Behav Biomed Mater. 2013;28:397-409.
Google Scholar | Crossref | Medline3. Wang, Y, Wang, X, Shi, J, et al. A biomimetic silk fibroin/sodium alginate composite scaffold for soft tissue engineering. Sci Rep. 2016;6:39477.
Google Scholar | Crossref | Medline4. Diogo, GS, Senra, EL, Pirraco, RP, et al. Marine collagen/apatite composite scaffolds envisaging hard tissue applications. Mar Drugs. 2018;16:E269.
Google Scholar | Crossref | Medline5. Lu, G, Ding, Z, Wei, Y, Lu, X, Lu, Q, Kaplan, DL. Anisotropic biomimetic silk scaffolds for improved cell migration and healing of skin wounds. ACS Appl Mater Interfaces. 2018;10:44314-44323.
Google Scholar | Crossref | Medline6. Lu, D, Wang, H, Wang, X, et al. Biomimetic chitosan-graft-polypeptides for improved adhesion in tissue and metal. Carbo-hydr Polym. 2019;215:20-28.
Google Scholar | Crossref | Medline7. Ojeh, NO, Frame, JD, Navsaria, HA. In vitro characterization of an artificial dermal scaffold. Tissue Eng. 2001;7:457-472.
Google Scholar | Crossref | Medline8. Heimbach, DM, Warden, GD, Luterman, A, et al. Multicenter postapproval clinical trial of Integra dermal regeneration template for burn treatment. J Burn Care Rehabil. 2003;24:42-48.
Google Scholar | Crossref | Medline9. Branski, LK, Herndon, DN, Pereira, C, et al. Longitudinal assessment of Integra in primary burn management: a randomized pediatric clinical trial. Crit Care Med. 2007;35:2615-2623.
Google Scholar | Crossref | Medline10. Xiong, S, Zhang, X, Lu, P, et al. A gelatin-sulfonated silk composte based scaffold based on the 3D printing technology enhances the skin regeneration by stimulating epidermal growth and dermal neovascularization. Sci Rep. 2017;7:4288.
Google Scholar | Crossref | Medline11. Jiang, S, Lyu, C, Zhao, P, et al. Cryoprotectant enables structural control of porous scaffolds for exploration of cellularmechano-responsiveness in 3D. Nat Commun. 2019;10:3491.
Google Scholar | Crossref | Medline12. Mulye, SP, Wadkar, KA, Kondawar, MS. Formulation, development and evaluation of indomethacin emulgel. Der Pharmacia Sinica. 2013;4:31-45.
Google Scholar13. Scholten, HJ, Pierik, RLM. Agar as a gelling agent: chemical and physical analysis. Plant Cell Rep. 1998;17:230-235.
Google Scholar | Crossref | Medline14. Martín-López, E, Darder, M, Ruiz-Hitzky, E, Sampedro, MN. Agar-based bridges as biocompatible candidates to provide guide cues in spinal cord injury repair. Biomed Mater Eng. 2013;23:405-421.
Google Scholar | Medline15. Uppuluri, VNVA, Shanmugarajan, TS. Icariin-loaded polyvinyl alcohol/agar hydrogel: development, characterization, and in vivo evaluation in a full-thickness burn model. Int J Low Extrem Wounds. 2019;18:323-335.
Google Scholar | SAGE Journals | ISI16. Ishii, F, Sasaki, I, Ogata, H. Effect of phospholipid emulsifiers on physicochemical properties of intravenous fat emulsions and/or drug carrier emulsions. J Pharm Pharmacol. 1990;42:513-515.
Google Scholar | Crossref | Medline17. Maier, C, Zeeb, B, Weiss, J. Investigations into aggregate formation with oppositely charged oil-in-water emulsions at different pH values. Colloids Surf B Biointerfaces. 2014;117:368-375.
Google Scholar | Crossref | Medline18. Chung, C, Koo, CKW, Sher, A, Fu, JR, Rousset, P, McClements, DJ. Modulation of caseinate-stabilized model oil-in-water emulsions with soy lecithin. Food Res Int. 2019;122:361-370.
Google Scholar | Crossref | Medline19. Gabás-Rivera, C, Barranquero, C, Martínez-Beamonte, R, Navarro, MA, Surra, JC, Osada, J. Dietary squalene increases high density lipoprotein-cholesterol and paraoxonase 1 and decreases oxidative stress in mice. PLoS One. 2014;9:e104224.
Google Scholar | Crossref | Medline20. Katsarou, AI, Kaliora, AC, Chiou, A, et al. Amelioration of oxidative and inflammatory status in hearts of cholesterol-fed rats supplemented with oils or oil-products with extra virgin olive oil components. Eur J Nutr. 2016;55:1283-1296.
Google Scholar | Crossref | Medline21. Pal, K, Banthia, AK, Majumdar, DK. Preparation and characterization of polyvinyl alcohol-gelatin hydrogel membranes for biomedical applications. AAPS PharmSciTech. 2007;8:21.
Google Scholar | Crossref | Medline22. Singh, VK, Pal, K, Pradhan, DK, Pramanik, K. Castor oil and sorbitan monopalmitate based organogel as a probable matrix for controlled drug delivery. J Appl Polym Sci. 2013;130:1503-1515.
Google Scholar | Crossref23. Lou-Bonafonte, JM, Martínez-Beamonte, R, Sanclemente, T, et al. Current insights into the biological action of squalene. Mol Nutr Food Res. 2018;8:e1800136.
Google Scholar | Crossref24. Sagiri, SS, Singh, VK, Kulanthaivel, S, et al. Stearate organogel-gelatin hydrogel based bigels: physicochemical, thermal, mechanical characterizations and in vitro drug delivery applications. J Mech Behav Biomed Mater. 2015;43:1-17.
Google Scholar | Crossref | Medline25. Shen, CY, Dai, L, Shen, BD, et al. Nanostructured lipid carrier based topical gel of Ganoderma triterpenoids for frostbite treatment. Chin J Nat Med. 2015;13:454-460.
Google Scholar | Medline26. Bera, H, Nadimpalli, J, Kumar, S, Vengala, P. Kondogogu gum-Zn+2-pectinate emulgel matrices reinforced with mesoporous silica for intragastric furbiprofen delivery. Int J Biol Macromol. 2017;104(pt A):1229-1237.
Google Scholar | Crossref | Medline27. Yang, H, Irudayaraj, J, Paradkar, MM. Discriminant analysis of edible oils and fats by FTIR. FT-NIR and FT-Raman spectroscopy. Food Chem. 2005;93:25-32.
Google Scholar | Crossref | ISI28. Pourjavadi, A, Farhadpour, B, Seidi, F. Synthesis and investigation of swelling behavior of new agar based superabsorbent hydrogel as a candidate for agrochemical delivery. J Polym Res. 2009;16:655-665.
Google Scholar | Crossref29. Pradhan, S, Sagiri, SS, Singh, VK, Pal, K, Ray, SS, Pradhan, DK. Palm oil-based organogels and microemulsions for delivery of antimicrobial drugs. J Appl Polym Sci. 2014;131:39979.
Google Scholar | Crossref30. Behera, B, Biswal, D, Uvanesh, K, et al. Modulating the properties of sunflower oil based novel emulgels using castor oil fatty acid ester: prospects for topical antimicrobial drug delivery. Colloids Surf B Biointerfaces. 2015;128:155-164.
Google Scholar | Crossref | Medline31. Salem, HF, Kharshoum, RM, Abou-Taleb, HA, Naguib, DM. Nanosized nasal emulgel of resveratrol: preparation, optimization, in vitro evaluation and in vivo pharmacokinetic study. Drug Dev Ind Pharm. 2019;9:1624-1634.
Google Scholar | Crossref32. Zhang, P, Chen, L, Zhang, Q, Hong, FF. Using in situ dynamic cultures to rapidly biofabricate fabric-reinforced composites of chitosan/bacterial nanocellulose for antibacterial wound dressings. Front Microbiol. 2016;4:260.
Google Scholar33. Maharana, V, Gaur, D, Nayak, SK, et al. Reinforcing the inner phase of the filled hydrogels with CNTs alters drug release properties and human keratinocyte morphology: a study on the gelatin-tamarind gum filled hydrogels. J Mech Behav Biomed Mater. 2017;75:538-548.
Google Scholar | Crossref | Medline34. Khamrai, M, Banerjee, SL, Paul, S, Samanta, S, Kundu, PP. Curcumin entrapped gelatin/ionically modified bacterial cellulose based self-healable hydrogel film: an eco-friendly sustainable synthesis method of wound healing patch. Int J Biol Macromol. 2019;122:940-953.
Google Scholar | Crossref | Medline35. Ali, NH, Amin, MCIM. Sodium carboxymethyl cellulose hydrogels containing reduced graphene oxide (rGO) as a functional antibiofilm wound dressing. J Biomater Sci Polym Ed. 2019;30:629-645.
Google Scholar | Crossref | Medline36. Gönüllü, Ü, Üner, M, Yener, G, Karaman, EF, Aydoğmuş, Z. Formulation and characterization of solid lipid nanoparticles, nanostructured lipid carriers and nanoemulsion of lornoxicam for transdermal delivery. Acta Pharm. 2015;65:1-13.
Google Scholar | Crossref | Medline37. Hussain, A, Samad, A, Singh, SK, et al. Nanoemulsion gel-based topical delivery of an antifungal drug: in vitro activity and in vivo evaluation. Drug Deliv. 2016;23:642-647.
Google Scholar | Crossref | Medline38. El-Refaie, WM, Elnaggar, YS, El-Massik, MA, Abdallah, OY. Novel curcumin-loaded gel-core hyaluosomes with promising burn-wound healing potential: development, in-vitro appraisal and in-vivo studies. Int J Pharm. 2015;486:88-98.
Google Scholar | Crossref | Medline39. Tian, M, Qing, C, Niu, Y, et al. The relationship between inflammation and impaired wound healing in a diabetic rat burn model. J Burn Care Res. 2016;37:e115-e124.
Google Scholar | Crossref | Medline | ISI40. Abreu, AM, Douglas, de, Oliveira, DW, Marinho, SA, Lima, NL, de Miranda, JL, Verli, FD. Effect of topical application of different substances on fibroplasia in cutaneous surgical wounds. ISRN Dermatol. 2012;2012:282973.
Google Scholar | Crossref | Medline41. Quinn, KP, Golberg, A, Broelsch, GF, et al. An automated image processing method to quantify collagen fibre organization within cutaneous scar tissue. Exp Dermatol. 2015;24:78-80.
Google Scholar | Crossref | Medline42. Oryan, A, Mohammadalipour, A, Moshiri, A, Tabandeh, MR. Topical application of Aloe vera accelerated wound healing, modeling, and remodeling: an experimental study. Ann Plast Surg. 2016;77:37-46.
Google Scholar | Crossref | Medline43. Alam, P, Ansari, MJ, Anwer, MK, Raish, M, Kamal, YK, Shakeel, F. Wound healing effects of nanoemulsion containing clove essential oil. Artif Cells Nanomed Biotechnol. 2017;45:591-597.
Google Scholar | Crossref | Medline44. Zhu, X, Hu, C, Zhang, Y, Li, L, Wang, Z. Expression of cyclin-dependent kinase inhibitors, p21cip1 and p27kip1, during wound healing in rats. Wound Repair Regen. 2001;9:205-212.
Google Scholar | Crossref | Medline45. Yang, C, Zhu, P, Yan, L, Chen, L, Meng, R, Lao, G. Dynamic changes in matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 levels during wound healing in diabetic rats. J Am Podiatr Med Assoc. 2009;99:489-496.
Google Scholar | Crossref | Medline46. Martin, LF, Rocha, EM, Garcia, SB, Paula, JS. Topical Brazilian propolis improves corneal wound healing and inflammation in rats following alkali burns. BMC Complement Altern Med. 2013;13:337.
Google Scholar | Crossref | Medline47. dos Santos, JS, Monte-Alto-Costa, A. Caffeic acid phenethyl ester improves burn healing in rats through anti-inflammatory and antioxidant effects. J Burn Care Res. 2013;34:682-688.
Google Scholar | Crossref | Medline48. Zheng, L, Hui, Q, Tang, L, et al. TAT-mediated acidic fibroblast growth factor delivery to the dermis improves wound healing of deep skin tissue in rat. PLoS One. 2015;10:e0135291.
Google Scholar | Crossref | Medline49. de Loura Santana, C, de Fátima Teixeira Silva, D, de Souza, AP, et al. Effect of laser therapy on immune cells infiltrate after excisional wounds in diabetic rats. Lasers Surg Med. 2016;48:45-51.
Google Scholar | Crossref | Medline50. Shen, HM, Chen, C, Jiang, JY, et al. The N-butyl alcohol extract from Hibiscus rosa-sinensis L. flowers enhances healing potential on rat excisional wounds. J Ethnopharmacol. 2017;198:291-301.
Google Scholar | Crossref | Medline

Comments (0)

No login
gif