yea3679-sup-0001-SupplementalNote1.docxWord 2007 document , 29.4 KB

Data S1. Note 1 - Detailed yeast enrichment, isolation, and identification protocol.

yea3679-sup-0002-Figure_S1.pdfPDF document, 177.4 KB

Figure S1. A) Histogram of isolates in the complete dataset. B) When the 1,962 isolates were filtered to remove duplicate OTUs derived from the same processed sample, 1,516 unique isolations remained (bottom).

yea3679-sup-0003-Figure_S2.pdfPDF document, 424.3 KB

Figure S2. The distribution of isolations in the dataset by climate region. The number of unique isolations (upper, bold) and the number of unique OTUs (lower, italics) are shown. Climate regions correspond to the 9 climatically consistent regions of the contiguous US identified by NOAA (https://www.ncdc.noaa.gov/monitoring-references/maps/us-climate-regions.php). We included the additional category of Arctic for Alaskan samples.

yea3679-sup-0004-Figure_S3.pdfPDF document, 122.2 KB

Figure S3. A) Rarefaction curve for 688 unique samples (black line) with 95% confidence intervals. Solid curve based on observed data and dashed curve based on extrapolated data. B) Rarefaction of 688 unique samples by the higher taxonomic units Basidiomycota (yellow), Pezizomycotina (blue), and Saccharomycotina (green). Solid curve based on observed data; dashed curve based on extrapolated data with shaded 95% confidence intervals.

yea3679-sup-0005-Figure_S4.pdfPDF document, 108.6 KB

Figure S4. Taxonomic representation across unique isolations (light grey, top count) and unique OTUs (dark grey, bottom count). The dearth of Pezizomycotina OTUs is likely reflective of our isolation protocol, in which the selection of mold-like colonies was deliberately avoided.

yea3679-sup-0006-Figure_S5.pdfPDF document, 997.5 KB

Figure S5. 103 singleton OTUs were isolated just once. A) Distribution of substrate categories among singletons. Singleton OTUs were not enriched for any substrate type. B) Distribution of singletons among higher taxonomic ranks. Singletons were not enriched for any taxonomic group. C) Isolation locations of singletons.

yea3679-sup-0007-Figure_S6.pdfPDF document, 6.7 KB

Figure S6. A) The number of independent isolations of each cosmopolitan OTU. B) The number of climatic regions in which each cosmopolitan OTU was detected.

yea3679-sup-0008-Figure_S7.pdfPDF document, 1.3 MB

Figure S7. Cosmopolitan OTUs were defined as those OTUs that were isolated from regions where they were expected based on sampling density (allowing for one missed region). Twelve cosmopolitan OTUs were identified using this approach. A) Cosmopolitan yeasts were enriched in soil samples (p = 0.003). B) Cosmopolitan yeasts all belong to Saccharomycotina or Basidiomycota, but there was no enrichment for taxonomic groups among cosmopolitan OTUs. C) Map of cosmopolitan isolation locations. OTUs are differentiated by color.

yea3679-sup-0009-Figure_S8.pdfPDF document, 1.6 MB

Figure S8. Forty discrete substrate categories were annotated for 1,520 isolations. A) Distribution of unique isolations among substrate categories. Category sampling was uneven. B) Substrate genera could be assigned to 1,061 isolations of diverse substrate categories. For each genus, the type of substrate collected is shown with different color bars. Substrate categories were either directly harvested from the substrate (e.g. pine needles from pine trees) or indirectly associated with the substrate (e.g. soil from the base of a pine tree).

yea3679-sup-0010-Figure_S9.pdfPDF document, 123.7 KB

Figure S9. A) Observed data fed into substrate association permutations. B) Observed data fed into substrate plant genus association permutations. C) Observed data fed into isolation temperature associations.

yea3679-sup-0011-Table_S1.xlsxExcel 2007 spreadsheet , 175.3 KB

Table S1 – Raw metadata for 1,962 isolates.

yea3679-sup-0012-Table_S2.xlsxExcel 2007 spreadsheet , 15.1 KB

Table S2 – OTUs isolated only once.

yea3679-sup-0013-Table_S3.xlsxExcel 2007 spreadsheet , 33 KB

Table S3 – OTUs isolated greater than 20 times.

yea3679-sup-0014-Table_S4.xlsxExcel 2007 spreadsheet , 17.5 KB

Table S4 – Discovery rates and regional expectations for 66 OTUs.

yea3679-sup-0015-Table_S5.xlsxExcel 2007 spreadsheet , 33.3 KB

Table S5 – Cosmopolitan OTUs.

yea3679-sup-0016-Table_S6.xlsxExcel 2007 spreadsheet , 13.8 KB

Table S6 – Contingency table for higher taxonomic group representation across substrate types.

yea3679-sup-0017-Table_S7.xlsxExcel 2007 spreadsheet , 70.7 KB

Table S7 – Permutation results for specific substrate – yeast OTU associations.

yea3679-sup-0018-Table_S8.xlsxExcel 2007 spreadsheet , 68.4 KB

Table S8 – Permutation results for substrate genus – yeast OTU associations.

yea3679-sup-0019-Table_S9.xlsxExcel 2007 spreadsheet , 18.7 KB

Table S9 – All isolates of Saccharomyces cerevisiae and Saccharomyces paradoxus.

yea3679-sup-0020-Table_S10.xlsxExcel 2007 spreadsheet , 9.1 KB

Table S10 – Contingency table for higher taxonomic group representation across isolation temperatures.

yea3679-sup-0021-Table_S11.xlsxExcel 2007 spreadsheet , 61.5 KB

Table S11 – Permutation results for isolation temperature – yeast OTU associations.

yea3679-sup-0022-Table_S12.xlsxExcel 2007 spreadsheet , 10.1 KB

Table S12 – Diversity indices for 4 substrate types.

yea3679-sup-0023-Table_S13.xlsxExcel 2007 spreadsheet , 10.2 KB

Table S13 – Diversity indices for 4 isolation temperatures.

yea3679-sup-0024-Table_S14.xlsxExcel 2007 spreadsheet , 11.2 KB

Table S14 - Jaccard distance matrix of isolation temperatures.

yea3679-sup-0025-Table_S15.xlsxExcel 2007 spreadsheet , 17.7 KB

Table S15 - ITS Regions of 57 isolates failing to meet GenBank submission criteria.