CRISPR-Cas9

Donor template

Homologous-Directed Repair (HDR)

PolyPurine Reverse Hoogsteen hairpins (PPRHs)

Repair template

Repairing strategy

Single-stranded oligodeoxynucleotide (ssODN)

Targeted Template Design

Triplex-forming oligonucleotide (TFO)

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