Jefferson T, Dooley L, Ferroni E, Al-Ansary LA, van Driel ML, Bawazeer GA, et al. Physical interventions to interrupt or reduce the spread of respiratory viruses. Cochrane Database Syst Rev. 2023;1(1):CD006207. https://doi.org/10.1002/14651858.CD006207.

Article  Google Scholar 

Schwartz JL. The Spanish Flu, epidemics, and the turn to biomedical responses. Am J Public Health. 2018;108(11):1455–8. https://doi.org/10.2105/AJPH.2018.304581.

Article  PubMed Central  Google Scholar 

van den Hoogen BG, de Jong JC, Groen J, Kuiken T, de Groot R, Fouchier RA, et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med. 2001;7(6):719–24. https://doi.org/10.1038/89098.

Article  CAS  PubMed Central  Google Scholar 

Du Y, Li W, Guo Y, Li L, Chen Q, He L, Shang S. Epidemiology and genetic characterization of human metapneumovirus in pediatric patients from Hangzhou China. J Med Virol. 2022;94(11):5401–8. https://doi.org/10.1002/jmv.28024.

Article  CAS  Google Scholar 

Divarathna MVM, Rafeek RAM, Noordeen F. A review on epidemiology and impact of human metapneumovirus infections in children using TIAB search strategy on PubMed and PubMed Central articles. Rev Med Virol. 2020;30(1): e2090. https://doi.org/10.1002/rmv.2090.

Article  Google Scholar 

Ogonczyk Makowska D, Hamelin MÈ, Boivin G. Engineering of live chimeric vaccines against human metapneumovirus. Pathogens. 2020;9(2):135. https://doi.org/10.3390/pathogens9020135.

Article  CAS  PubMed Central  Google Scholar 

Tang X, Dai G, Wang T, Sun H, Jiang W, Chen Z, et al. Comparison of the clinical features of human bocavirus and metapneumovirus lower respiratory tract infections in hospitalized children in Suzhou. China Front Pediatr. 2023;10:1074484. https://doi.org/10.3389/fped.2022.1074484.

Article  Google Scholar 

Amarasinghe GK, Aréchiga Ceballos NG, Banyard AC, Basler CF, Bavari S, Bennett AJ, et al. Taxonomy of the order Mononegavirales: update 2018. Arch Virol. 2018;163(8):2283–94. https://doi.org/10.1007/s00705-018-3814-x.

Article  CAS  PubMed Central  Google Scholar 

Ye H, Zhang S, Zhang K, Li Y, Chen D, Tan Y, et al. Epidemiology, genetic characteristics, and association with meteorological factors of human metapneumovirus infection in children in southern China: a 10-year retrospective study. Int J Infect Dis. 2023;137:40–7. https://doi.org/10.1016/j.ijid.2023.10.002.

Article  CAS  Google Scholar 

Yi L, Zou L, Peng J, Yu J, Song Y, Liang L, et al. Epidemiology, evolution and transmission of human metapneumovirus in Guangzhou China, 2013–2017. Sci Rep. 2019;9(1):14022. https://doi.org/10.1038/s41598-019-50340-8.

Article  ADS  CAS  PubMed Central  Google Scholar 

Tulloch RL, Kok J, Carter I, Dwyer DE, Eden JS. An amplicon-based approach for the whole-genome sequencing of human metapneumovirus. Viruses. 2021;13(3):499. https://doi.org/10.3390/v13030499.

Article  CAS  PubMed Central  Google Scholar 

Roussy JF, Carbonneau J, Ouakki M, Papenburg J, Hamelin MÈ, De Serres G, et al. Human metapneumovirus viral load is an important risk factor for disease severity in young children. J Clin Virol. 2014;60(2):133–40. https://doi.org/10.1016/j.jcv.2014.03.001.

Article  Google Scholar 

Otomaru H, Nguyen HAT, Vo HM, Toizumi M, Le MN, Mizuta K, et al. A decade of human metapneumovirus in hospitalized children with acute respiratory infection: molecular epidemiology in central Vietnam, 2007–2017. Sci Rep. 2023;13(1):15757.

Article  ADS  CAS  PubMed Central  Google Scholar 

Ma S, Zhu F, Xu Y, Wen H, Rao M, Zhang P, et al. Development of a novel multi-epitope mRNA vaccine candidate to combat HMPV virus. Hum Vaccin Immunother. 2023;19(3):2293300. https://doi.org/10.1080/21645515.2023.2293300.

Article  CAS  Google Scholar 

Van Den Bergh A, Bailly B, Guillon P, von Itzstein M, Dirr L. Antiviral strategies against human metapneumovirus: Targeting the fusion protein. Antiviral Res. 2022;207: 105405. https://doi.org/10.1016/j.antiviral.

Article  Google Scholar 

Gálvez NMS, Andrade CA, Pacheco GA, Soto JA, Stranger V, Rivera T, et al. Host components that modulate the disease caused by hMPV. Viruses. 2021;13(3):519. https://doi.org/10.3390/v13030519.

Article  CAS  PubMed Central  Google Scholar 

Kim JI, Park S, Lee I, Park KS, Kwak EJ, Moon KM, et al. Genome-wide analysis of human metapneumovirus evolution. PLoS ONE. 2016;11(4): e0152962. https://doi.org/10.1371/journal.pone.0152962.

Article  CAS  PubMed Central  Google Scholar 

Percivalle E, Sarasini A, Visai L, Revello MG, Gerna G. Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies. J Clin Microbiol. 2005;43(7):3443–6. https://doi.org/10.1128/JCM.43.7.3443-3446.2005.

Article  PubMed Central  Google Scholar 

Okamoto M, Sugawara K, Takashita E, Muraki Y, Hongo S, Mizuta K, et al. Development and evaluation of a whole virus-based enzyme-linked immunosorbent assay for the detection of human metapneumovirus antibodies in human sera. J Virol Methods. 2010;164(1–2):24–9. https://doi.org/10.1016/j.jviromet.2009.11.019.

Article  CAS  Google Scholar 

Liu L, Bastien N, Sidaway F, Chan E, Li Y. Seroprevalence of human metapneumovirus (hMPV) in the Canadian province of Saskatchewan analyzed by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay. J Med Virol. 2007;79(3):308–13. https://doi.org/10.1002/jmv.20799.

Article  CAS  Google Scholar 

Ishiguro N, Akutsu Y, Azuma K, Yonekawa M, Sato D, Ishizaka A, et al. Evaluation of a novel immunochromatographic assay using monoclonal antibodies against the matrix protein of human metapneumovirus. Clin Lab. 2021;67(10):1. https://doi.org/10.7754/Clin.Lab.2021.210232.

Article  Google Scholar 

Cong S, Wang C, Wei T, Xie Z, Huang Y, Tan J, et al. Human metapneumovirus in hospitalized children with acute respiratory tract infections in Beijing, China. Infect Genet Evol. 2022;106: 105386. https://doi.org/10.1016/j.meegid.2022.105386.

Article  CAS  Google Scholar 

Wang C, Wei T, Ma F, Wang H, Guo J, Chen A, et al. Epidemiology and genotypic diversity of human metapneumovirus in paediatric patients with acute respiratory infection in Beijing, China. Virol J. 2021;18(1):40. https://doi.org/10.1186/s12985-021-01508-0.

Article  CAS  PubMed Central  Google Scholar 

Li J, Mao NY, Zhang C, Yang MJ, Wang M, Xu WB, et al. The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes. BMC Infect Dis. 2012;12:189. https://doi.org/10.1186/1471-2334-12-189.

Article  CAS  PubMed Central  Google Scholar 

Guo X, Cui YX, Zhuge SR, Liu XM. Comparative study on real-time PCR and RT-PCR testing methods for detecting human metapneumovirus. Guiyang Med Coll J. 2015;40:834–8 ((in Chinese)).

Google Scholar 

Lu JR, Zhang LL, Tan WJ, Zhou WM, Wang Z, Peng K, et al. Development and application of real-time RT-PCR assay for detection of human metapneumovirus in Beijing. Lett Biotechnol. 2008;19:207–9 ((in Chinese)).

CAS  Google Scholar 

Sugimoto S, Kawase M, Suwa R, Kakizaki M, Kume Y, Chishiki M, et al. Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube. J Virol Methods. 2023;322: 114812. https://doi.org/10.1016/j.jviromet.2023.114812.

Article  CAS  Google Scholar 

You HL, Chang SJ, Yu HR, Li CC, Chen CH, Liao WT. Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms. BMC Pediatr. 2017;17(1):89. https://doi.org/10.1186/s12887-017-0843-7.

Article  CAS  PubMed Central  Google Scholar 

Garbuglia AR, Lapa D, Pauciullo S, Raoul H, Pannetier D. Nipah virus: an overview of the current status of diagnostics and their role in preparedness in endemic countries. Viruses. 2023;15(10):2062. https://doi.org/10.3390/v15102062.

Article  CAS  PubMed Central  Google Scholar 

Feng ZS, Zhao L, Wang J, Qiu FZ, Zhao MC, Wang L, et al. A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus. Virol J. 2018;15(1):167. https://doi.org/10.1186/s12985-018-1061-0.

Article  CAS  PubMed Central  Google Scholar 

Xu X, Cai L, Liang S, Zhang Q, Lin S, Li M, Y, et al. Digital microfluidics for biological analysis and applications. Lab Chip. 2023;23(5):1169–91. https://doi.org/10.1039/d2lc00756h.

Article  CAS  Google Scholar 

Huang H, Huang K, Sun Y, Luo D, Wang M, Chen T, et al. A digital microfluidic RT-qPCR platform for multiple detections of respiratory pathogens. Micromachines (Basel). 2022;13(10):1650. https://doi.org/10.3390/mi13101650.

Article  Google Scholar 

Flores-Contreras EA, Carrasco-González JA, Linhares DCL, Corzo CA, Campos-Villalobos JI, Henao-Díaz A, et al. Emergent molecular techniques applied to the detection of porcine viruses. Vet Sci. 2023;10(10):609. https://doi.org/10.3390/vetsci10100609.

Article  PubMed Central  Google Scholar 

Song Q, Zhu R, Sun Y, Zhao L, Wang F, Deng J, et al. Identification of human metapneumovirus genotypes A and B from clinical specimens by reverse transcription loop-mediated isothermal amplification. J Virol Methods. 2014;196:133–8. https://doi.org/10.1016/j.jviromet.2013.10.037.

Article  CAS  Google Scholar 

Wang X, Zhang Q, Zhang F, Ma F, Zheng W, Zhao Z, et al. Visual detection of the human metapneumovirus using reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye. Virol J. 2012;9:138. https://doi.org/10.1186/1743-422X-9-138.

Article  CAS  PubMed Central  Google Scholar 

Wang D, Wang Y, Zhu K, Shi L, Zhang M, Yu J, et al. Detection of cassava component in sweet potato noodles by real-time loop-mediated isothermal amplification (real-time LAMP) method. Molecules. 2019;24(11):2043. https://doi.org/10.3390/molecules24112043.

Article  CAS  PubMed Central