The amazing characteristics of light have made its use for a range of multipurpose in every science discipline, including plant sciences. Among different types of light, depending on its properties, laser light has also gained great an interest for different purposes, including its use in biological objects for useful outcomes [[1], [2], [3]] when is used in low intensities. It is now clear that light energy, including of laser when given to biological objects is stored and is then later on converted to biochemical energy during different metabolic activities but up to specific energy limits because high energy lights including lasers have damaging effects to the biological objects. Though the high energy lights are also being used to alter the metabolism to induce mutation for including characteristics especially in crop plants and multiple successes has made in this regard [4,5]. However, it is well known that in biological objects the given light in low intensities is absorbed in the form of photons, by specific cellular components that change the biochemical potential of cellular membranes [1,3,6,7] . For example regarding the light energy absorption related processes, taking place in biological objects, including the absorption of photons by phytochrome C oxidases in mitochondria specific for ATP production as well as by chloroplastic components, also derive the energy related processes [8,9]. Absorption of lights by these components result in more ATP production, as a result speedy biological cellular metabolic processes, especially the activities of metabolic enzymes [2,[10], [11], [12], [13]] . Similar is in case of laser light application [2,7,14]. However, now it is well-known that the positive and negative effects are laser dose and plant species specific [2,7,15,16,17] . Not only the positive influences but also the negative impacts of laser light application have also been reported [3,18,19] . Furthermore, studies also revealing that both low and high energy laser found beneficial as their use in biological processes [15,20,21] . A well-known effect of laser light application in biological or living processes is the generation of free radicals due to absorption of light energy that start a cascade of biological reactions, responsible for the generation of reactive oxygen species (ROS) that also lead to the production of secondary radicals [10,22,23] . These ROS not only act as signaling molecules in low concentration to start a series of biological processes but also has sever damaging effects in high concentrations to different cellular membranes known as lipid peroxidation and also damage the macromolecules such as DNA, enzymes, proteins etc. that also retard the basic beneficial biological processes, necessary for normal plant growth [22,24,25] . Over production of ROS also changes the stable redox state of cell [23,26] that results in cellular pH changes [27,28], as well as damages the cellular membranes and disturbs the cellular metabolic processes [26,29] . Different types of lasers have also gained great interest of the researchers as physical methods for beneficial and environment friendly uses in biological disciplines, including in agriculture for better crop production by boosting seed germination to obtaine better crop stand through their use as seed treatment [2,7,14–16] . Application of laser as seed irradiation improves various characteristics of plants such as seed germination, root development, improvement in photosynthetic pigments and at later growth stages increase in leaf area, grain yield and quality [2,7,30]. However, the effective doses of lasers used in crop plants for the betterment of crops was found plant species specific (Perveen et al. 2010; Jamil et al. 2013; Perveen et al., 2021a,b; Heinemann et al., 2022[2,7,14,17,24,], type of laser [2,7] and plant parameter specific [22,25]. Most of the studies revealed the positive influences of lasers used as seed treatment but the effects were plant species specific and only very few studies revealed the negative influences as seed treatment. Moreover, mostly the available studies are about the use of continuous wave HeNe laser, CO2 and diode lasers but very few has been reported regarding the use of Nd:YAG pulsed laser in high or low doses. For example, Ling et al. [31]) reported that the dry seeds of peanut were irradiated with Nd:YAG laser (1060 nm) for 8 s. They found that in the root tips of seedling the aberrations were increased from 0.16 to 0.53% when an exposure increased from 3 s to 8 s dose and the 8 s dose was found lethal one, while that of 3 s was found positive one in terms of growth increments. Regarding Dunaliella sativa, Jie et al. [32]), and Sarreta and de Castro [33]), irradiated the seeds with Nd:YAG, LD and Ar+ lasers to find out their effects on different germination attributes and pigment accumulation in seedlings. They reported that Nd:YAG pulsed laser adversely effected the carotenoid accumulation, while LD and Ar+ lasers improved its contents. However, the effects were laser dose and time of irradiation specific. Moreover, in earlier studies the use of Nd:YAG pulsed laser was done on the dry seeds and the effects were studied only on germination and seedling stages, not on later growth stages, regarding photosynthetic activity and morphological changes. The objective of present study was to find out the influences of a range of Nd:YAG pulsed laser doses (low to high) as kernel treatment on kernel germination, seedling survival rate, leaf morphology, photosynthetic pigments and different gas exchange attributes. As earlier studies revealing that the most of the earlier work is on HeNe or CO2 continuous wave low energy laser and very little work has been reported about the use of a range of Nd:YAG pulsed laser doses as seed treatment irradiation to examine the impacts on growth at later stages in relation with leaf photosynthetic efficiency and photosynthetic pigments under field conditions. So, in view of the information available in literature regarding the use of lasers in plants, it was hypothesized that the maize kernel treatment with a range of Nd:YAG lasers might produce the amazing and interesting results. Moreover, mostly the studies regarding the impacts of laser seed treatment are performed in growth room under controlled conditions and very rare work was found reported under field conditions to find out the laser induced modulations in plant morphological and physiological attributes as were the objectives of the present study.